期刊
LANGMUIR
卷 27, 期 14, 页码 8923-8933出版社
AMER CHEMICAL SOC
DOI: 10.1021/la200837z
关键词
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资金
- National Science Foundation [CHE-0848375]
- Army Research Office through the Institute for Collaborative Biotechnologies at UCSB [W911NF-09-D-0001]
- U.S. Department of Energy, Office of Basic Energy Sciences user facility at Los Alamos National Laboratory [DE-AC52-06NA25396]
- Sandia National Laboratories [DE-AC04-94AL85000]
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [0848375] Funding Source: National Science Foundation
Silver DNA nanoclusters (Ag:DNAs) are novel fluorophores under active research and development as alternative biomolecular markers. Comprised of a few-atom Ag cluster that is stabilized in water by binding to a strand of DNA, they are also interesting for fundamental explorations into the properties of metal molecules. Here, we use in situ calibrated electrokinetic microfluidics and fluorescence correlation spectroscopy to determine the size, charge, and conformation of a select set of Ag:DNAs. Among them is a pair of spectrally distinct Ag:DNAs stabilized by the same DNA sequence, for which it is known that the silver cluster differs by two atoms. We find these two Ag:DNAs differ in size by similar to 30%, even though their molecular weights differ by less than 3%. Thus a single DNA sequence can adopt very different conformations when binding slightly different Ag clusters. By comparing spectrally identical Ag:DNAs that differ in sequence, we show that the more compact conformation is insensitive to the native DNA secondary structure. These results demonstrate electrokinetic microfluidics as a practical tool for characterizing Ag:DNA.
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