期刊
LANGMUIR
卷 27, 期 11, 页码 6987-6994出版社
AMER CHEMICAL SOC
DOI: 10.1021/la1047358
关键词
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资金
- Welch Foundation [E-1498]
- NSF [CTS-0349228, DMR-0706627]
- NIH [R21EY018303]
- Alliance for NanoHealth [W81XWH-09-2-0139]
- W.M. Keck Center for Interdisciplinary Bioscience Training of the Gulf Coast Consortia (NIH) [5R90 DK71504-3]
Micro- and nanopatterns of biomolecules on inert, ultrathin platforms on nonoxidized silicon are ideal interfaces between silicon-based microelectronics and biological systems. We report here the local oxidation nanolithography with conductive atomic force microscopy (cAFM) on highly protein-resistant, oligo(ethylene glycol) (OEG)-terminated alkyl monolayers on nonoxidized silicon substrates. We propose a mechanism for this process, suggesting that it is possible to oxidize only the top ethylene glycol units to generate carboxylic acid and aldehyde groups on the film surface. We show that avidin molecules can be attached selectively to the oxidized pattern and the density can be varied by altering the bias voltage during cAFM patterning. Biotinylated molecules and nanoparticles are selectively immobilized on the resultant avidin patterns. Since one of the most established methods for immobilization of biomolecules is based on avidin-biotin binding and a wide variety of biotinylated biomolecules are available, this approach represents a versatile means for prototyping any nanostructures presenting these biomolecules on silicon substrates.
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