期刊
LANGMUIR
卷 26, 期 11, 页码 7675-7678出版社
AMER CHEMICAL SOC
DOI: 10.1021/la101192v
关键词
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资金
- National Institutes of Health [DK069275]
- Juvenile Diabetes Research Foundation
- Whitaker Foundation
We report herein a new and enabling approach for decorating both abiotic and cell surfaces with the extracellular matrix IKVAV peptide in a site-specific manner using strain promoted azide-alkyne cycloaddition. A cyclooctyne-derivatized IKVAV peptide was synthesized and immobilized on the surface of pancreatic islets through strain-promoted azide-alkyne cycloaddition with cell surface azides generated by the electrostatic adsorption of a cytocompatible poly(L-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG) copolymer bearing azido groups (PP-N-3). Both one-pot and sequential addition of PP-N3 and a cyclooctyne-derivatized IKVAV peptide conjugate enabled efficient modification of the pancreatic islet surface in less than 60 min. The ability to bind peptides at controlled surface densities was demonstrated in a quantitative manner using microarrays. Additionally, the technique is remarkably rapid and highly efficient, opening new avenues or the molecular engineering of cellular interfaces and protein and peptide microarrays.
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