Review
Oncology
Hyeon-Yeol Cho, Jin-Ha Choi, Joungpyo Lim, Sang-Nam Lee, Jeong-Woo Choi
Summary: The review highlights the significance of metastasis as the main cause of cancer-related death and discusses recent advances in the detection and analysis of circulating tumor cells and circulating cancer stem cells. The focus is on the design of microfluidic devices with optical sensing tools and strategies for analyzing the differentiation of circulating cancer stem cells.
Article
Genetics & Heredity
In Hee Lee, Gyeonghwa Kim, Sang Gyu Kwak, Dong Won Baek, Byung Woog Kang, Hye Jin Kim, Su Yeon Park, Jun Seok Park, Gyu-Seog Choi, Keun Hur, Jong Gwang Kim
Summary: The study validated candidate biomarkers for predicting LN metastasis in colon cancer patients using plasma miRNA concentrations, identifying three circulating miRNAs significantly associated with LN metastasis.
Article
Medicine, General & Internal
Farah J. Nassar, Zahraa S. Msheik, Maha M. Itani, Remie El Helou, Ruba Hadla, Firas Kreidieh, Rachelle Bejjany, Deborah Mukherji, Ali Shamseddine, Rihab R. Nasr, Sally N. Temraz
Summary: CRC is the second leading cause of cancer deaths worldwide, with poor prognosis for Stage IV patients and liver metastasis as the main cause of mortality. miRNA shows potential as non-invasive diagnostic biomarkers for CRC, with specific panels of miRNA having diagnostic accuracy for advanced CRC and liver metastasis.
Article
Oncology
Sukanya Basu, Yang Dong, Rahul Kumar, Collene Jeter, Dean G. Tang
Summary: Cancer cell heterogeneity and plasticity pose significant challenges to effective clinical treatments. Research has shown that cancer cells can exhibit stem-like characteristics and undergo dynamic changes during tumor progression. In addition, tumors also contain a population of non-proliferative cells that are resistant to anti-mitotic drugs. Understanding these unique cell populations in tumors can lead to the development of new therapeutic strategies to prevent relapse and metastasis.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Biology
Tong Xu, Mathijs Verhagen, Rosalie Joosten, Wenjie Sun, Andrea Sacchetti, Leonel Munoz Sagredo, Veronique Orian-Rousseau, Riccardo Fodde
Summary: Phenotypic plasticity plays a crucial role in allowing carcinoma cells to acquire transient mesenchymal features for invasion and metastasis. Epithelial-to-mesenchymal (EMT) and mesenchymal-to-epithelial (MET) transitions are regulated by a broad range of epigenetic mechanisms, including alternative splicing (AS). In this study, we focus on the role of AS in eliciting phenotypic plasticity in colon cancer and identify specific RNA-binding proteins (RBPs) that alter AS patterns, leading to the generation of splicing isoforms associated with increased invasion and metastasis. Further validation studies show that these RBPs and splicing isoforms promote local invasion and distant metastasis in colon cancer and are associated with poor survival.
Review
Biochemical Research Methods
Ramon Lorenzo-Gomez, Rebeca Miranda-Castro, Noemi De-los-Santos-alvarez, Maria Jesus Lobo-Castanon
Summary: The role of extracellular matrix (ECM) in tumorigenesis and metastasis is crucial, as tumor cells need to recruit a permissive microenvironment for growth. The interaction between tumor cells and ECM leads to the release of ECM-derived molecules into the bloodstream, which can serve as potential biomarkers for tumor development. Developing better bioanalytical methods for detecting ECM remodeling-derived components is essential for translating basic knowledge about cancer progression into clinical practice.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Linglong Peng, Yongfu Xiong, Rong Wang, Ling Xiang, He Zhou, Zhongxue Fu
Summary: PRDX2 is highly expressed in colon cancer stem cells (CCSCs) and its overexpression is closely correlated with stemness maintenance, tumor initiation, and invasion of CCSCs. Inhibition of PRDX2 leads to increased ROS production in CCSCs, sensitizing them to oxidative stress and chemotherapy. These findings suggest that PRDX2 could be a potential therapeutic target in CCSCs.
Article
Oncology
Taohua Yue, Jichang Li, Jing Zhu, Shuai Zuo, Xin Wang, Yucun Liu, Jia Liu, Xiaoyun Liu, Pengyuan Wang, Shanwen Chen
Summary: Immunotherapy can be effective in treating cancer, but many colorectal cancer patients do not respond to it. This study shows that reducing H2S levels can help reverse the tumor-supportive functions and improve the efficacy of immunotherapy.
Article
Medicine, General & Internal
Kehe Chen, Zhenxiang Chen, Mei Ou, Junping Wang, Xiao Huang, Yingying Wu, Wenhe Zhong, Jiao Yang, Jinging Huang, Min Huang, Deng Pan
Summary: This study suggests that the number of circulating tumor cells (CTCs) can serve as a useful biomarker for predicting the prognosis of colorectal cancer patients. Patients with higher CTCs or MCTCs count exhibit poorer progression-free survival (PFS) and overall survival (OS) rates.
Article
Multidisciplinary Sciences
Yuki Ohta, Masayuki Fujii, Sirirat Takahashi, Ai Takano, Kosaku Nanki, Mami Matano, Hikaru Hanyu, Megumu Saito, Mariko Shimokawa, Shingo Nishikori, Yoshiko Hatano, Ryota Ishii, Kazuaki Sawada, Akihito Machinaga, Wataru Ikeda, Takeshi Imamura, Toshiro Sato
Summary: This study develops an experimental platform for tracking cancer stem cells in colorectal cancer and identifies dormant LGR5(+) cancer stem cells. Transcriptome analysis reveals an upregulation of cell-adhesion molecule COL17A1 in dormant cancer stem cells, and chemotherapy disrupts this dormancy through FAK-YAP activation. Inhibition of YAP signaling prevents chemoresistant cells from exiting dormancy and delays tumor regrowth.
Review
Oncology
Yunus Akkoc, Nesibe Peker, Arzu Akcay, Devrim Gozuacik
Summary: Metastasis and relapse are the main causes of cancer-related deaths. Dormant cancer cells are able to evade conventional treatments, with autophagy playing a crucial role in the adaptation, survival, and reactivation of these cells.
FRONTIERS IN ONCOLOGY
(2021)
Review
Medicine, General & Internal
Zicheng Deng, Shengming Wu, Yilong Wang, Donglu Shi
Summary: Circulating tumor cells (CTCs) play a crucial role in cancer diagnosis and prognosis. Current detection technologies have limitations due to tumor heterogeneity, but a new technique using superparamagnetic nanoprobe shows higher sensitivity and biomarker independence.
Article
Oncology
Shengbo Han, Decai Wang, Yan Huang, Zhu Zeng, Peng Xu, Hewei Xiong, Zunxiang Ke, Ya Zhang, Yuhang Hu, Fan Wang, Jie Wang, Yong Zhao, Wenfeng Zhuo, Gang Zhao
Summary: Research has shown that the emergence of Schwann cells around premalignant lesions of colon cancer may be an early indicator promoting tumorigenesis. This study explored the communication between colon cancer cells and Schwann cells, and identified NGF and exosomal miR-21-5p as potential therapeutic targets for colon cancer treatment.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Oncology
Utsav Sharma, Kelsie Medina-Saenz, Philip C. Miller, Benjamin Troness, Angela Spartz, Ana Sandoval-Leon, Deanna N. Parke, Tiffany N. Seagroves, Marc E. Lippman, Dorraya El-Ashry
Summary: The study revealed that the clustering of circulating CAFs and CTCs occurs early in BC progression and is present in all clinical stages of BC. Heterotypic cCAFs-CTC clusters have a higher metastatic potential compared to homotypic CTC clusters. The adhesion and stemness marker CD44 plays a significant role in the heterotypic clustering of cCAFs and BC cells, enhancing their metastatic potential. These findings highlight the impact of circulating cell clusters on BC metastasis.
BREAST CANCER RESEARCH AND TREATMENT
(2021)
Article
Medicine, General & Internal
Omneya Attallah, Muhammet Fatih Aslan, Kadir Sabanci
Summary: Lung and colon cancers are leading causes of mortality and morbidity. This study proposes a framework based on multiple lightweight deep learning models to detect these cancers at an early stage, achieving a high accuracy of 99.6% through feature reduction and fusion techniques.
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)