期刊
LABORATORY INVESTIGATION
卷 92, 期 10, 页码 1492-1502出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/labinvest.2012.109
关键词
CARS; Coherent anti-stokes Raman scattering; histology; in vivo microscopy; SRS; stimulated Raman scattering
资金
- Boehringer Ingelheim Fonds
- Army Research Office
- Italian Society of Multiple Sclerosis (FISM) [Cod 2008/B/5]
- NIH [R01NS049263]
- NSF [0958345]
- NIH/NIBIB [1R01EB010244-01]
- Direct For Computer & Info Scie & Enginr
- Division Of Computer and Network Systems [0958345] Funding Source: National Science Foundation
Conventional histopathology with hematoxylin & eosin (H&E) has been the gold standard for histopathological diagnosis of a wide range of diseases. However, it is not performed in vivo and requires thin tissue sections obtained after tissue biopsy, which carries risk, particularly in the central nervous system. Here we describe the development of an alternative, multicolored way to visualize tissue in real-time through the use of coherent Raman imaging (CRI), without the use of dyes. CRI relies on intrinsic chemical contrast based on vibrational properties of molecules and intrinsic optical sectioning by nonlinear excitation. We demonstrate that multicolor images originating from CH2 and CH3 vibrations of lipids and protein, as well as two-photon absorption of hemoglobin, can be obtained with subcellular resolution from fresh tissue. These stain-free histopathological images show resolutions similar to those obtained by conventional techniques, but do not require tissue fixation, sectioning or staining of the tissue analyzed. Laboratory Investigation (2012) 92, 1492-1502; doi:10.1038/labinvest.2012.109; published online 20 August 2012
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