期刊
LABORATORY INVESTIGATION
卷 92, 期 10, 页码 1503-1514出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/labinvest.2012.114
关键词
Aurora kinase; CBB2001; geminin; mitosis; Plk1 kinase
资金
- National Natural Science Foundation of China (NSFC) [30971616, 21133002]
- Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality (SZSITIC) [JC201104210125A]
- Pfizer (China)-PKU
Polo-like kinase 1 (Plk1) is a mitotic serine/threonine kinase and its kinase activity is closely interrelated to cell cycle progression, various types of cancer development and often correlates with poor prognosis. Thus, it is of prime importance to characterize the phenotypes of Plk1 inhibition in cells for drug development and clinical application. Here, we report a novel kinase inhibitory chemical, CBB2001, which specifically inhibited Plk1 kinase activity in vitro with an IC50 of 0.39 mu M. In cervical carcinoma HeLa cells, we found that treatment of CBB2001 caused mitotic cell cycle arrest (EC50 = 0.72 mu M) and induction of 'polo' cells (EC50 = 0.32 mu M). Interestingly, the cell cycle arrest induced by CBB2001 was associated with accumulation of Plk1 (EC50 = 0.61 mu M) and Geminin (EC50 = 0.43 mu M) proteins, but distinct from the phenotypes induced by Aurora kinase inhibitors. The inhibitory effects of CBB2001 were phenocopied by RNA interferences of Plk1. We also confirmed the cell cycle inhibitory effects of CBB2001 in other cancer cells. Moreover, CBB2001 inhibited the growth of He La cells with an IC50 of 0.85 mu M in MU assays, which is better than that of reported Plk1 inhibitory chemicals ON01910 (IC50 = 6.46 mu M) and LFM-A13 (IC50 = 37.36 mu M). CBB2001 also inhibited mouse xenograft tumor growth. Furthermore, CBB2001 inhibited mitotic exit and delayed degradation of APC/C substrates, Geminin, Cyclin B1 and Aurora A. These specific phenotypes may serve as specific features for Plk1 inhibition and for Plk1-based clinic trials. Laboratory Investigation (2012) 92, 1503-1514; doi:10.1038/labinvest.2012.114; published online 13 August 2012
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