Article
Oncology
I. Gusti Md Gde Surya C. Trapika, Xin Tracy Liu, Long Hoa Chung, Felcia Lai, Chanlu Xie, Yang Zhao, Shaohui Cui, Jinbiao Chen, Collin Tran, Qian Wang, Shubiao Zhang, Anthony S. Don, George Qian Li, Jane R. Hanrahan, Yanfei Qi
Summary: Prostate cancer is the second most prevalent malignancy worldwide, thus novel treatments are urgently needed. Research shows that erianin exhibits anti-tumor effects in both androgen-sensitive and castration-resistant prostate cancer cells through different mechanisms.
FRONTIERS IN ONCOLOGY
(2021)
Article
Nutrition & Dietetics
Kok-Lun Pang, Lian-Chee Foong, Norzana Abd Ghafar, Ima Nirwana Soelaiman, Jia Xian Law, Lek Mun Leong, Kok-Yong Chin
Summary: This study investigated the anticancer properties of annatto tocotrienol (AnTT), gamma-tocotrienol (gamma-T3), and delta-tocotrienol (delta-T3) on chondrosarcoma cells. The results showed that these tocotrienols induced G1 arrest and apoptosis in the cells, as well as caused cytoplasmic vacuolation. Transcriptomic analysis revealed upregulated signaling pathways related to endoplasmic reticulum stress, autophagy, and transcription. Several cell proliferation and cancer-related pathways, such as Hippo and Wnt signaling pathways, were also downregulated. Further research is needed to confirm the effectiveness of these tocotrienols as adjuvant therapy for chondrosarcoma.
Article
Pharmacology & Pharmacy
Yuan Wang, Jinfeng Cui, Guona Zheng, Man Zhao, Zengfang Hao, Hongguang Lian, Yuehong Li, Wenxin Wu, Xianghong Zhang, Juan Wang
Summary: This study provides new insights into the molecular mechanisms underlying the cytotoxicity of Ochratoxin A in gastric cells, mainly involving the ROS-mediated endoplasmic reticulum stress pathway and subsequent cell cycle arrest, apoptosis, and autophagy.
Article
Cell Biology
Michelle Joy-Immediato, Manuel J. Ramirez, Mauricio Cerda, Yusuke Toyama, Andrea Ravasio, Pakorn Kanchanawong, Cristina Bertocchi
Summary: Cadherin-mediated adhesions are adhesive complexes that connect neighboring cells in a tissue. The extension of the ER to these adhesions is tension dependent and cadherin-type specific. The structural organization of the ER/microtubule network can affect the localization of ER-bound PTP1B at these adhesions, potentially modulating the mechanotransduction of adherens junctions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Lingling Li, Piao Li, Wei Zhang, Haiting Zhou, Ergang Guo, Guoqing Hu, Linli Zhang
Summary: In this study, it was found that FERMT1 was upregulated in NPC tissues and correlated with the clinicopathological characteristics of NPC patients. Knockdown of FERMT1 significantly decreased cell proliferation, migration, and invasion by mediating epithelial-mesenchymal transition (EMT) and cell cycle arrest of NPC cells.
CANCER CELL INTERNATIONAL
(2022)
Article
Environmental Sciences
Mengping Yu, Feipeng Chen, Haopei Wang, Qianlei Fu, Lingzi Yan, Zhao Chen, Huijun Li, Miaomiao Jia, Dalong Yang, Xiaohui Hua, Tong Shen, Qixing Zhu, Chengfan Zhou
Summary: This study found that nickel chloride can induce endoplasmic reticulum stress and unfolded protein response in lung cancer cells, leading to epithelial-mesenchymal transition and cell migration. Inhibition of endoplasmic reticulum stress can reverse nickel ion-induced migration and transformation, and this process may be related to the activation of Smads and MAPKs signaling pathways.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Lin Fu, Hui Zhao, Ying Xiang, Hui-Xian Xiang, Biao Hu, Zhu-Xia Tan, Xue Lu, Lan Gao, Bo Wang, Hua Wang, Cheng Zhang, De-Xiang Xu
Summary: Chronic exposure to 1-nitropyrene (1-NP) induces pulmonary fibrosis in mice, potentially through oxidative stress-induced endoplasmic reticulum stress leading to epithelial-mesenchymal transition.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biochemistry & Molecular Biology
Fan Lin, Chengcheng Liao, Jinsheng Zhang, Yun Sun, Weiwei Lu, Yu Bai, Yixuan Liao, Minxia Li, Yongfen Qi, Yahong Chen
Summary: This study found that EMT and ER stress occurred in lung epithelial cells in smokers and COPD patients. NaHS significantly alleviated CS-induced lung tissue damage, small airway fibrosis, ER stress, and EMT, while the cystathionine-gamma-lyase inhibitor aggravated these effects. An appropriate concentration of H2S donor inhibited nicotine-induced ER stress, cell migration ability, and EMT. The IRE1 signal pathway and Smad2/3 may be responsible for the inhibitory effect of H2S.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Pharmacology & Pharmacy
Wen-Wen Wang, Ying-Lu Liu, Mei-Zi Wang, Huan Li, Bu-Hui Liu, Yue Tu, Can-Can Yuan, Qi-Jun Fang, Jia-Xin Chen, Jie Wang, Yan Fu, Zi-Yue Wan, Yi-Gang Wan, Wei Wu
Summary: The study confirmed that Shenkang injection (SKI) can alleviate diabetic tubulopathy (DT) in a similar manner as rosiglitazone (ROS), and SKI achieves this effect by inhibiting epithelial-to-mesenchymal transition (EMT) and endoplasmic reticulum (ER) stress-induced apoptosis.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Ophthalmology
Jiayi Wei, Liangjing Wu, Shuai Yang, Conghui Zhang, Le Feng, Minli Wang, Hui Li, Fang Wang
Summary: Proliferative vitreoretinopathy (PVR) is a serious ocular fibrotic disease that can lead to blindness. This study focuses on the pathogenesis of PVR and the mechanism behind the epithelial-mesenchymal transition (EMT) in retinal pigment epithelial (RPE) cells. The results show that the loss of E-cadherin leads to changes in the catenin-cadherin complex, activating zinc finger E-box binding homeobox 1 (ZEB1) and promoting the upregulation of N-cadherin. Overexpression of E-cadherin inhibits the EMT process and cell proliferation in RPE cells.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Immunology
Mingyu Liu, Chong Wu, Shufeng Luo, Qiaomin Hua, Hai-Tian Chen, Yulan Weng, Junyu Xu, Huiling Lin, Lu Wang, Jinheng Li, Lan Zhu, Zhenhong Guo, Shi-Mei Zhuang, Tiebang Kang, Limin Zheng
Summary: The study reveals that PKR-like endoplasmic reticulum kinase (PERK) plays a crucial role in hematopoietic stem/progenitor cell (HSPC) preconditioning and the generation of tumor-promoting myeloid cells in the spleen. Inhibiting PERK signaling pathway prevents HSPCs from becoming MDSCs, providing a potential therapeutic opportunity for restraining the tumor-promoting myeloid response.
JOURNAL OF EXPERIMENTAL MEDICINE
(2022)
Article
Oncology
Xiaoxiao Meng, Kan Liu, Hui Xie, Yong Zhu, Wei Jin, Jian Lu, Ruilan Wang
Summary: The study revealed that ER stress may promote EMT through the PERK signaling pathway in PQ-induced pulmonary fibrosis. Silencing PERK can suppress PQ-induced EMT and the progression of pulmonary fibrosis.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Multidisciplinary Sciences
Najat Bouchmaa, Reda Ben Mrid, Youssef Bouargalne, Sana Ajouaoi, Francesco Cacciola, Rachid El Fatimy, Mohamed Nhiri, Abdelmajid Zyad
Summary: Women's breast cancer is a significant healthcare issue and requires proactive research for a cure. This study evaluated the cytotoxic activity of two natural steroidal compounds, protodioscin and dioscin, against two major subtypes of breast cancer. Both compounds showed high cytotoxicity against the tested cell lines and decreased the clonogenic capacity. Dioscin also reduced cell motility. These compounds induce oxidative stress and decrease enzymatic activities, making them promising for treating breast cancer.
Article
Biochemistry & Molecular Biology
Theodoros Eleftheriadis, Georgios Pissas, Spyridon Golfinopoulos, Maria Efthymiadi, Christina Poulianiti, Maria Anna Polyzou Konsta, Vassilios Liakopoulos, Ioannis Stefanidis
Summary: This study evaluated the response of renal proximal tubular epithelial cells (RPTECs) to high albumin concentration, focusing on the unfolded protein response (UPR) and DNA damage response (DDR). The results showed that albumin led to reactive oxygen species (ROS) overproduction and protein modification, which activated both UPR and DDR. This resulted in cellular apoptosis, senescence, and epithelial-to-mesenchymal transition (EMT). While agents against endoplasmic reticulum stress (ERS) only partially alleviated the harmful effects, inhibiting ROS upregulation prevented both UPR and DDR. Therefore, suppressing ROS overproduction may be more effective in mitigating the deleterious effects of albumin overload.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Zuxiao Chen, Haiyan Li, Zongyan Li, Shaoyi Chen, Xiaoming Huang, Zheyu Zheng, Xiangjun Qian, Lei Zhang, Guojie Long, Jiancong Xie, Qian Wang, Weidong Pan, Dawei Zhang
Summary: Tumor-associated macrophages (TAMs) in cholangiocarcinoma regulate their function through the SHH signaling pathway and endoplasmic reticulum stress (ERS). Cholangiocarcinoma cells regulate TAM polarization and TGF-beta 1 secretion, while TAMs promote the growth and migration of cholangiocarcinoma cells through TGF-beta 1.
PHARMACOLOGICAL RESEARCH
(2023)
Review
Oncology
Mary Kay Washington, Richard M. Goldberg, George J. Chang, Paul Limburg, Alfred K. Lam, Manuel Salto-Tellez, Mark J. Arends, Iris D. Nagtegaal, David S. Klimstra, Massimo Rugge, Peter Schirmacher, Alexander J. Lazar, Robert D. Odze, Fatima Carneiro, Masashi Fukayama, Ian A. Cree
Summary: The WHO Classification of Tumours provides international standards for classification and diagnosis, with the latest fifth edition going digital and including a series of books. This article summarizes major diagnostic innovations from the past decade that have been incorporated into the classification.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Pathology
Lisa Browning, Eve Fryer, Derek Roskell, Kieron White, Richard Colling, Jens Rittscher, Clare Verrill
Summary: The COVID-19 pandemic has put pressure on our diagnostic services, and the use of digital pathology is on the rise globally, playing a role in maintaining diagnostic practices.
JOURNAL OF CLINICAL PATHOLOGY
(2021)
Review
Pathology
Lisa Browning, Richard Colling, Emad Rakha, Nasir Rajpoot, Jens Rittscher, Jacqueline A. James, Manuel Salto-Tellez, David R. J. Snead, Clare Verrill
Summary: The measures to control COVID-19 outbreak will likely continue until a suitable vaccine or treatment is found, impacting clinical services and research, with pathologists working remotely and changes to protocols and studies. Digital pathology and artificial intelligence may play a key role in safeguarding clinical services and pathology-based research in the current and future climate.
JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Oncology
Sudhir B. Malla, David J. Fisher, Enric Domingo, Andrew Blake, Sylvana Hassanieh, Keara L. Redmond, Susan D. Richman, Michael Youdell, Steven M. Walker, Gemma E. Logan, Aikaterina Chatzipli, Raheleh Amirkhah, Matthew P. Humphries, Stephanie G. Craig, Ultan McDermott, Matthew T. Seymour, Dion G. Morton, Philip Quirke, Nicholas P. West, Manuel Salto-Tellez, Richard D. Kennedy, Patrick G. Johnston, Ian Tomlinson, Viktor H. Koelzer, Letitia Campo, Richard S. Kaplan, Daniel B. Longley, Mark Lawler, Timothy S. Maughan, Louise C. Brown, Philip D. Dunne
Summary: Positivity of DDIR does not predict improved response to oxaliplatin treatment in colorectal cancer, with DDIR-negative patients showing a trend towards better outcomes. The DDIR assay may have potential in identifying immune-rich tumors that could benefit from immune checkpoint blockade therapies, beyond the current use of MSI status.
CLINICAL CANCER RESEARCH
(2021)
Article
Oncology
Jun Li, Mercedes A. Duran, Ninjit Dhanota, Walid K. Chatila, Sarah E. Bettigole, John Kwon, Roshan K. Sriram, Matthew P. Humphries, Manuel Salto-Tellez, Jacqueline A. James, Matthew G. Hanna, Johannes C. Melms, Sreeram Vallabhaneni, Kevin Litchfield, Ieva Usaite, Dhruva Biswas, Rohan Bareja, Hao Wei Li, Maria Laura Martin, Princesca Dorsaint, Julie-Ann Cavallo, Peng Li, Chantal Pauli, Lee Gottesdiener, Benjamin J. DiPardo, Travis J. Hollmann, Taha Merghoub, Hannah Y. Wen, Jorge S. Reis-Filho, Nadeem Riaz, Shin-San Michael Su, Anusha Kalbasi, Neil Vasan, Simon N. Powell, Jedd D. Wolchok, Olivier Elemento, Charles Swanton, Alexander N. Shoushtari, Eileen E. Parkes, Benjamin Izar, Samuel F. Bakhoum
Summary: ENPP1 promotes metastasis by degrading cGAMP, inhibiting immune cell infiltration, increasing metastatic potential, and inducing resistance to immunotherapy in chromosomally unstable tumors.
Article
Oncology
Fatima Abdullahi Sidi, Victoria Bingham, Stephanie G. Craig, Stephen McQuaid, Jacqueline James, Matthew P. Humphries, Manuel Salto-Tellez
Summary: The study demonstrates the usefulness of advanced computer software in helping pathologists assess routine tests for immunotherapy treatment. Multiplex testing can provide specific information from patient samples. The use of multiplex immunofluorescence and digital image analysis is effective in quantifying PD-L1 in non-small cell lung cancer and supports clinical workflows.
Article
Biochemistry & Molecular Biology
M. P. Humphries, P. Maxwell, M. Salto-Tellez
Summary: QuPath, created at Queen's University Belfast, is the most widely used image analysis software program globally, addressing various needs in tissue-based image analysis and serving as the system of choice for researchers in scientific research.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Gastroenterology & Hepatology
Jonathan W. J. Lee, Feng Zhu, Supriya Srivastava, Stephen Kk Tsao, Christopher Khor, Khek Yu Ho, Kwong Ming Fock, Wee Chian Lim, Tiing Leong Ang, Wan Cheng Chow, Jimmy Bok Yan So, Calvin J. Koh, Shijia Joy Chua, Andrew S. Y. Wong, Jaideepraj Rao, Lee Guan Lim, Khoon Lin Ling, Chung-King Chia, Choon Jin Ooi, Andrea Rajnakova, Wai Ming Yap, Manuel Salto-Tellez, Bow Ho, Richie Soong, Kee Seng Chia, Yik Ying Teo, Ming Teh, Khay-Guan Yeoh
Summary: This study found that gastric intestinal metaplasia (IM) is a significant risk factor for early gastric neoplasia (EGN) in regions with low-intermediate incidence of gastric cancer. Based on the results, it is recommended that high-risk patients (OLGIM III-IV) undergo endoscopic surveillance every 2 years, and intermediate-risk patients (OLGIM II) every 5 years.
Article
Pathology
Ross J. Porter, Graeme Murray, Abdo Alnabulsi, Matthew P. Humphries, Jacqueline A. James, Manuel Salto-Tellez, Stephanie G. Craig, Ji M. Wang, Teizo Yoshimura, Mairi H. McLean
Summary: Research reveals that loss of cathelicidin is associated with human CRC progression, with a switch in expression intensity as an early feature. LL-37 expression intensity is linked to CD8(+) T cell infiltrate and influenced by tumor characteristics.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2021)
Article
Pathology
Abdo Alnabulsi, Tiehui Wang, Wei Pang, Marius Ionescu, Stephanie G. Craig, Matthew P. Humphries, Kris McCombe, Manuel Salto Tellez, Ayham Alnabulsi, Graeme Murray
Summary: A study developed an effective methodology to identify optimal biomarker combinations for the prognosis of colorectal carcinoma. Six biomarkers were identified as the best combination in terms of prognostic power, and it was significantly associated with overall survival and independent of other clinicopathological parameters. The study also demonstrated the potential of this methodology for biomarker discovery in various biological and clinical studies.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2022)
Article
Oncology
Vivek Kumar Singh, Md Mostafa Kamal Sarker, Yasmine Makhlouf, Stephanie G. Craig, Matthew P. Humphries, Maurice B. Loughrey, Jacqueline A. James, Manuel Salto-Tellez, Paul O'Reilly, Perry Maxwell
Summary: In this study, a lightweight deep learning model called ICOSeg is proposed to accurately segment the immune-checkpoint biomarker ICOS protein in colon cancer immunohistochemistry slide patches. The proposed model utilizes a convolutional neural network and transformer to extract relevant features from immunohistochemistry images and performs segmentation using an encoder and decoder sub-network. Experimental results show that ICOSeg achieves better segmentation results compared to existing methods with a reduction in parameters.
Article
Pathology
Matthew Phillip Humphries, Victoria Bingham, Fatima Abdullah Sidi, Stephanie Craig, Beatrize Lara, Hesham El-daly, Nicole O'Doherty, Perry Maxwell, Claire Lewis, Stephen McQuaid, James Lyness, Jacqueline James, David R. J. Snead, Manuel Salto-Tellez
Summary: In this study, a validated protocol using multiplex immunofluorescence (mIF) was described for the exploration of the molecular physiopathology of SARS-CoV-2 pulmonary disease. Validated assays originally developed for the detection of SARS-CoV-2 in tissues were applied to map the adaptive immune response at protein and mRNA levels. The mIF panels showed robust, reliable, and quantifiable performance in detecting topographic variations in inflammation related to pathological processes in formalin-fixed, paraffin-embedded pneumonia material.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Editorial Material
Oncology
Manuel Salto-Tellez, Jorge S. Reis-Filho
Review
Biochemistry & Molecular Biology
Alice Geaney, Paul O'Reilly, Perry Maxwell, Jacqueline A. James, Darragh Mcart, Manuel Salto-Tellez
Summary: Digital pathology and artificial intelligence have transformed oncology research and cancer diagnostics, allowing for better interpretation of pathology images and improved quantitation of biomarkers. Different approaches of artificial intelligence can solve various problems in pathology workflows and have the potential to revolutionize clinical trials and create complex biomarkers for cancer patients.
Article
Biochemistry & Molecular Biology
Kris D. McCombe, Stephanie G. Craig, Amelie Viratham Pulsawatdi, Javier I. Quezada-Marin, Matthew Hagan, Simon Rajendran, Matthew P. Humphries, Victoria Bingham, Manuel Salto-Tellez, Richard Gault, Jacqueline A. James
Summary: The growth of digital pathology has led to advancements in cancer prediction and prognosis research through the use of deep learning and computer vision techniques. HistoClean, a user-friendly application, aims to improve deep learning models by providing transparent image pre-processing and augmentation techniques.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)