Article
Biochemistry & Molecular Biology
Szu-Hsien Wu, Yu-Ting Liao, Chi-Han Huang, Yi-Chou Chen, En-Rung Chiang, Jung-Pan Wang
Summary: ADSCs tend to spontaneously differentiate into neuron-like cells during high confluence culture, with enhanced expression of neuronal markers and neurotrophins, while BMSCs do not show this tendency. Additionally, ADSCs do not exhibit spontaneous adipogenic and osteogenic differentiation during this process.
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Cell Biology
Dawoon Baek, Kwang Hwan Park, Kyoung-Mi Lee, Sujin Jung, Soyeong Joung, Jihyun Kim, Jin Woo Lee
Summary: The study revealed that overexpression of USP53 can enhance osteogenic differentiation of hBMSCs by promoting the degradation of β-catenin through interaction with FBXO31, potentially promoting bone regeneration. Therefore, USP53 may be a promising target for gene therapy in bone regeneration.
CELL DEATH & DISEASE
(2021)
Article
Cell & Tissue Engineering
Jeroen van de Peppel, Gerben J. Schaaf, Adriana Arruda Matos, Yuan Guo, Tanja Strini, Wenda Verschoor, Amel Dudakovic, Andre J. van Wijnen, Johannes P. T. M. van Leeuwen
Summary: This research suggests that the cell surface glycoprotein CD24 could serve as a selective biomarker for identifying subpopulations of BMSCs with varying osteogenic potential. CD24(pos) cells have increased expression of genes related to cell adhesion, motility, and extracellular matrix, while also showing reduced proliferation compared to CD24(neg) cells.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell Biology
Lili Chang, He Gao, Li Wang, Ning Wang, Shumei Zhang, Xiaona Zhou, Huijun Yang
Summary: The study found that miR-1228 is highly expressed in serum exosomes of gastric cancer patients, and up-regulation of miR-1228 can down-regulate MMP-14 expression, effectively hindering the development of gastric cancer.
Article
Biochemistry & Molecular Biology
Zahra Eydian, Alaleh Mohammad Ghasemi, Samira Ansari, Ali Naghi Kamali, Maryam Khosravi, Sima Momtaz, Sanaz Riki, Laleh Rafighdoost, Reza Entezari Heravi
Summary: This study demonstrates the differentiation of mesenchymal stem cells (MSCs) from human adipose tissue and bone marrow into functional beta-islet cells. It suggests that adipose stem cells may be a better choice for the treatment of diabetes mellitus due to their higher potency.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Medicine, Research & Experimental
Ying Zhang, Xiangyang Cao, Peifeng Li, Yanan Fan, Leilei Zhang, Xianghao Ma, Ruibo Sun, Youwen Liu, Wuyin Li
Summary: The study demonstrates that BMSC-exo carrying miR-935 promotes osteoblast proliferation and differentiation through targeting STAT1.
Article
Biochemistry & Molecular Biology
Hyun Hee Jang, Youngsook Son, Gabee Park, Ki-Sook Park
Summary: This study demonstrates that bone marrow-derived mesenchymal stem cells (MSCs) have vasculogenic properties. Different populations of MSCs vary in angiogenic factor expression and vasculogenic properties. The vasculogenic MSCs (vMSCs), distinct from endothelial progenitor cells (EPCs), may contribute to neovascularization and could be a promising cellular therapy for cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Mintra Kaewkittikhun, Nittaya Boonmuen, Pakpoom Kheolamai, Sirikul Manochantr, Chairat Tantrawatpan, Nareerat Sutjarit, Duangrat Tantikanlayaporn
Summary: Andrographolide (AP) reduces lipid droplet accumulation in adipocytes by inhibiting adipogenic differentiation, suppressing adipogenic marker gene expression, and decreasing adipokine secretion levels, suggesting its potential as a novel therapeutic agent for obesity and related diseases.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Polymer Science
Liang Chen, Guoping Cheng, Shu Meng, Yi Ding
Summary: Fish scale collagen membrane, prepared with a high denaturation temperature and composed of a natural collagen composite, demonstrated good thermal stability, cytocompatibility, and osteogenic activity.
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Medicine, General & Internal
Tao Yang, Wei Li, Anquan Peng, Jia Liu, Qin Wang
Summary: In this study, it was found that hypoxic BMSCs-derived exosomes (hypBMSC-Exo) could alleviate cisplatin-induced ototoxicity and had better therapeutic effects compared to BMSC-Exo. This may be achieved by up-regulating the expression of SOD1 and SOD2 to protect auditory function.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Medicinal
Duaa Abuarqoub, Sofia Adwan, Rand Zaza, Suha Wehaibi, Nazneen Aslam, Hanan Jafar, Nidal Qinnah, Abdalla Awidi
Summary: Diabetes Mellitus Type 1 is an autoimmune disease that destroys insulin-producing cells, leading to hyperglycemia. This study examined the differentiation potential of dental stem cells (SCAP) and bone-marrow-derived stem cells (BM-MSCs) into functional islet cell aggregates (ICAs). The results showed that both types of stem cells successfully differentiated into beta-cell-like cells.
Article
Cell Biology
Huijiao Lv, Tao Wang, Shangkun Zhai, Zhuocheng Hou, Sirui Chen
Summary: Using transcriptomic analysis, we elucidated the transcriptomic changes during the osteogenic differentiation of chicken BMSCs at different time points. DEGs were mainly enriched in cell proliferation pathways during the early stage of osteogenic differentiation and pathways related to TGF-beta signaling during the middle and late stages. This study provides the first insight into the dynamic transcriptome changes during BMSC differentiation into osteoblasts in chickens.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Zinaida A. Dubeykovskaya, Phaneendra Kumar Duddempudi, Huan Deng, Giovanni Valenti, Krystle L. Cuti, Karan Nagar, Yagnesh Tailor, Chandan Guha, Jan Kitajewski, Timothy C. Wang
CANCER GENE THERAPY
(2019)
Review
Gastroenterology & Hepatology
Sama I. Sayin, Theresa Baumeister, Timothy C. Wang, Michael Quante
DIGESTIVE DISEASES AND SCIENCES
(2018)
Article
Cell Biology
Carolina Pinzon-Guzman, Anne R. Meyer, Rachel Wise, Eunyoung Choi, Sureshkumar Muthupalani, Timothy C. Wang, James G. Fox, James R. Goldenring
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Stefan Harmsen, Stephan Rogalla, Ruimin Huang, Massimiliano Spaliviero, Volker Neuschmelting, Yoku Hayakawa, Yoomi Lee, Yagnesh Tailor, Ricardo Toledo-Crow, Jeon Woong Kang, Jason M. Samii, Hazem Karabeber, Ryan M. Davis, Julie R. White, Matt van de Rijn, Sanjiv S. Gambhir, Christopher H. Contag, Timothy C. Wang, Moritz F. Kircher
Article
Gastroenterology & Hepatology
Yoku Hayakawa, Mayo Tsuboi, Samuel Asfaha, Hiroto Kinoshita, Ryota Niikura, Mitsuru Konishi, Masahiro Hata, Yukiko Oya, Woosook Kim, Moritz Middelhoff, Yohko Hikiba, Naoko Higashijima, Sozaburo Ihara, Tetsuo Ushiku, Masashi Fukayama, Yagnesh Tailor, Yoshihiro Hirata, Chandan Guha, Kelley S. Yan, Kazuhiko Koike, Timothy C. Wang
Editorial Material
Gastroenterology & Hepatology
Timothy C. Wang, Martin G. Martin, Calvin J. Kuo, Ophir D. Klein, Joyce Niland
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Mara Roxana Rubinstein, Jung Eun Baik, Stephen M. Lagana, Richard P. Han, William J. Raab, Debashis Sahoo, Piero Dalerba, Timothy C. Wang, Yiping W. Han
Article
Oncology
Jorge L. Sepulveda, Elena Komissarova, Sarawut Kongkarnka, Richard A. Friedman, Jon M. Davison, Brynn Levy, Diana Bryk, Vaidehi Jobanputra, Armando Del Portillo, Gary W. Falk, Joshua R. Sonett, Charles J. Lightdale, Julian A. Abrams, Timothy C. Wang, Antonia R. Sepulveda
INTERNATIONAL JOURNAL OF CANCER
(2019)
Article
Oncology
Haisheng Zhang, Antje Schaefer, Yichen Wang, Richard G. Hodge, Devon R. Blake, J. Nathaniel Diehl, Alex G. Papageorge, Matthew D. Stachler, Jennifer Liao, Jin Zhou, Zhong Wu, Fahire G. Akarca, Leonie K. de Klerk, Sarah Derks, Mariaelena Pierobon, Katherine A. Hoadley, Timothy C. Wang, George Church, Kwok-Kin Wong, Emanuel F. Petricoin, Adrienne D. Cox, Douglas R. Lowy, Channing J. Der, Adam J. Bass
Article
Gastroenterology & Hepatology
Henrik Nienhuser, Woosook Kim, Ermanno Malagola, Tuo Ruan, Giovanni Valenti, Moritz Middelhoff, Adam Bass, Channing J. Der, Yoku Hayakawa, Timothy C. Wang
Summary: The study revealed that gastric Mist1+ isthmus cells are activated in response to injury and inflammation, playing a crucial role in the regeneration of gastric glands through the Wnt5a pathway.
Article
Gastroenterology & Hepatology
Magdalena Kasendra, Misty Troutt, Taylor Broda, W. Clark Bacon, Timothy C. Wang, Joyce C. Niland, Michael A. Helmrath
Summary: Recent advances in intestinal organoid research have shown promising therapeutic potential for treating severely injured or diseased bowels using induced pluripotent stem cell (iPSC)-derived organoids. However, careful planning, significant resources, and collaboration between experts from various disciplines are required to address the safety and efficacy challenges in transitioning from laboratory discovery to first-in-human clinical trials.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2021)
Letter
Gastroenterology & Hepatology
Woosook Kim, Timothy C. Wang
Editorial Material
Biochemistry & Molecular Biology
Ermanno Malagola, Yoku Hayakawa, Timothy C. Wang
Summary: Recent research has identified distinct roles for Lgr4 and Lgr5 in directing gland reconstitution following H. pylori infection, shedding new light on the complexities of Rspo signaling during gastric regeneration and raising questions about antral stem cell hierarchy.
Article
Cell Biology
Toni T. Lemmetyinen, Emma W. Viitala, Linnea Wartiovaara, Tuomas Kaprio, Jaana Hagstrom, Caj Haglund, Pekka Katajisto, Timothy C. Wang, Eva Domenech-Moreno, Saara Ollila
Summary: Growth factors secreted by stromal fibroblasts, such as NRG1 and EREG, play a crucial role in regulating the intestinal epithelium. NRG1 promotes epithelial regeneration and protects against radiation-induced damage, while also suppressing the stem cell pool and remodeling the actin cytoskeleton. Interestingly, NRG1 does not support the growth of pre-tumorigenic intestinal organoids lacking the tumor suppressor Apc, suggesting a tumor-suppressive function.
DISEASE MODELS & MECHANISMS
(2023)
Article
Oncology
Jung Eun Baik, Li Li, Manish A. Shah, Daniel E. Freedberg, Zhezhen Jin, Timothy C. Wang, Yiping W. Han
Summary: Fusobacterium nucleatum (Fn) is a common gram-negative anaerobic bacterium that promotes colorectal cancer development through the secretion of FadAc. This study found that circulating levels of anti-FadAc IgA were significantly increased in patients with colorectal cancer, particularly in those with proximal tumors. Therefore, anti-FadAc IgA may serve as a serological biomarker for early detection of colorectal cancer.
CANCER RESEARCH COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Marijn A. Scheijde-Vermeulen, Lennart A. Kester, Liset Westera, Bastiaan B. J. Tops, Friederike A. G. Meyer-Wentrup
Summary: This study aimed to evaluate the feasibility of integrating state-of-the-art sequencing techniques and flow cytometry into the diagnostic workup of pediatric lymphoma. The results showed that this integration is not only feasible but also provides additional diagnostic information.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Enrico Berrino, Sara Erika Bellomo, Anita Chesta, Paolo Detillo, Alberto Bragoni, Amedeo Gagliardi, Alessio Naccarati, Matteo Cereda, Gianluca Witel, Anna Sapino, Benedetta Bussolati, Gianni Bussolati, Caterina Marchi
Summary: Formalin-fixed paraffin-embedded (FFPE) samples are crucial for tissue-based analysis in precision medicine, but the quality of these samples can affect the reliability of sequencing data. The use of acid-deprived fixatives guarantees the highest DNA preservation and sequencing performance, enabling more complex molecular profiling of tissue samples.
LABORATORY INVESTIGATION
(2024)
Article
Medicine, Research & Experimental
Roope A. Kallionpaa, Sirkku Peltonen, Kim My Le, Eija Martikkala, Mira Jaaskelainen, Elnaz Fazeli, Pilvi Riihila, Pekka Haapaniemi, Anne Rokka, Marko Salmi, Ilmo Leivo, Juha Peltonen
Summary: This study investigated the immune microenvironment of cutaneous neurofibromas (cNFs) in patients with neurofibromatosis 1 (NF1). The results showed that cNFs have substantial populations of T cells and macrophages, which may be tumor-specific. T cell populations in cNFs were found to be different from those in the skin, and cNFs exhibited lower expression of proteins related to T cell-mediated immunity compared to the skin.
LABORATORY INVESTIGATION
(2024)