期刊
LAB ON A CHIP
卷 14, 期 5, 页码 916-923出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc51139a
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资金
- National Institutes of Health [1DP2OD007209-01]
- National Science Foundation [ECCS-0824183, ECCS-0801922]
- Penn State Center for Nanoscale Science (MRSEC) [DMR-0820404]
- NHLBI Division of Intramural Research
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1346440] Funding Source: National Science Foundation
The development of microfluidic chip-based cytometers has become an important area due to their advantages of compact size and low cost. Herein, we demonstrate a sheathless microfluidic cytometer which integrates a standing surface acoustic wave (SSAW)-based microdevice capable of 3D particle/cell focusing with a laser-induced fluorescence (LIF) detection system. Using SSAW, our microfluidic cytometer was able to continuously focus microparticles/cells at the pressure node inside a microchannel. Flow cytometry was successfully demonstrated using this system with a coefficient of variation (CV) of less than 10% at a throughput of similar to 1000 events s(-1) when calibration beads were used. We also demonstrated that fluorescently labeled human promyelocytic leukemia cells (HL-60) could be effectively focused and detected with our SSAW-based system. This SSAW-based microfluidic cytometer did not require any sheath flows or complex structures, and it allowed for simple operation over a wide range of sample flow rates. Moreover, with the gentle, bio-compatible nature of low-power surface acoustic waves, this technique is expected to be able to preserve the integrity of cells and other bioparticles.
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