期刊
LAB ON A CHIP
卷 14, 期 24, 页码 4688-4695出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc01168f
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资金
- NSF [1253060]
- NIH [T32 HL007829]
- NIH NCATS [UL1TR000050, NIH-R01-GM094220]
- Chicago Biomedical Consortium
- Searle Funds at the Chicago Community Trust
- Div Of Biological Infrastructure
- Direct For Biological Sciences [1253060] Funding Source: National Science Foundation
An open-well microfluidic platform generates an oxygen landscape using gas-perfused networks which diffuse across a membrane. The device enables real-time analysis of cellular and tissue responses to oxygen tension to define how cells adapt to heterogeneous oxygen conditions found in the physiological setting. We demonstrate that localized hypoxic activation of cells elicited specific metabolic and gene responses in human microvascular endothelial cells and bone marrow-derived mesenchymal stem cells. A robust demonstration of the compatibility of the device with standard laboratory techniques demonstrates the wide utility of the method. This platform is ideally suited to study real-time cell responses and cell-cell interactions within physiologically relevant oxygen landscapes.
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