期刊
LAB ON A CHIP
卷 14, 期 10, 页码 1686-1694出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc51411k
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资金
- Natural Sciences and Engineering Research Council (NSERC) of Canada
- Natural Science Foundation of China [61174010]
- Shanxi Provincial government (100-talents program)
A diagnosis platform based entirely on DVD technology was developed for on-site quantitation of molecular analytes of interest, e.g., human chorionic gonadotropin (hCG) in urine samples (quantitative pregnancy test on a disc). An hCG-specific monoclonal antibody-binding assay prepared on a regular DVD-R was labeled with nanogold-streptavidin conjugates for signal enhancement with a customized silver-staining protocol. An unmodified, conventional computer optical drive was used for assay reading, and free disc-quality analysis software for data processing. The performance (sensitivity and selectivity) of this DVD assay is comparable to that of well-established colorimetric methods (determination of optical darkness ratios) and standard enzyme-linked immunosorbent assays (ELISA). As validated by examining its linear correlation with the ELISA results on the same set of samples, the DVD assay promises to be a low-cost, multiplex, point-of-care (POC) diagnostic tool for physicians and even for individuals at home, producing prompt results.
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