期刊
LAB ON A CHIP
卷 14, 期 16, 页码 2918-2928出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4lc00464g
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- Engineering and Physical Sciences Research Council [EP/L013983/1] Funding Source: researchfish
- EPSRC [EP/L013983/1] Funding Source: UKRI
We present a new concept for rapid and fully portable prostate specific antigen (PSA) measurements, termed lab-in-a-briefcase, which integrates an affordable microfluidic ELISA platform utilising a melt-extruded fiuoropolymer microcapillary film (MCF) containing an array of 10 200 mu m internal diameter capillaries, a disposable multi-syringe aspirator (MSA), a sample tray pre-loaded with all of the required immunoassay reagents, and a portable film scanner for colorimetric signal digital quantification. Each MSA can perform 10 replicate microfluidic immunoassays on 8 samples, allowing 80 measurements to be made in less than 15 minutes based on semi-automated operation, without the need of additional fluid handling equipment. The assay was optimised for the measurement of a clinically relevant range of PSA of 0.9 to 60.0 ng ml(-1) in 15 minutes with CVs on the order of 5% based on intra-assay variability when read using a consumer flatbed film scanner. The PSA assay performance in the MSA remained robust in undiluted or 1:2 diluted human serum or whole blood, and the matrix effect could simply be overcome by extending sample incubation times. The PSA lab-in-a-briefcase is particularly suited to a low-resource health setting, where diagnostic labs and automated immunoassay systems are not accessible, by allowing PSA measurement outside the laboratory using affordable equipment.
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