期刊
LAB ON A CHIP
卷 13, 期 7, 页码 1257-1265出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3lc41059e
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- NSBRI under NASA [NCC9-58]
Leukocyte differential count is one of the most frequently ordered clinical tests in hospitals. This paper reports a point-of-care test for the leukocyte count by using a microflow cytometer and a fluorescent dye assay. The dye assay relied on fluorescent detection alone to count leukocytes in blood and to identify leukocyte subtypes. By combining the fluorescent assay with a sheathless microflow design, the proposed method achieved a minimal sample volume by eliminating excessive dilution and sheath flow. In this paper, a four-part leukocyte differential count including lymphocyte, monocyte, neutrophil and eosinophil was demonstrated, and the whole test consumed only a small amount of blood (5 mu L) and reagents (68 mu L in total). The merits of minimal sample volume, long reagent shelf life and portable instrument made this method optimal for point-of-care applications.
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