期刊
LAB ON A CHIP
卷 12, 期 5, 页码 892-900出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20351g
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资金
- Institute of Bioengineering and Nanotechnology (HYU)
- Institute of Molecular and Cell Biology (VK)
- Biomedical Research Council, Agency for Science, Technology and Research (A*STAR) of Singapore [R-185-001-045-305]
- Ministry of Education [R-185-000-135-112]
- National Medical Research Council [R-185-000-099-213]
- Singapore-MIT Alliance [C-382-641-001-091]
- Janssen Cilag [R-185-000-182-592]
- Mechanobiology Institute of Singapore [R-714-001-003-271]
We have developed a multi-channel microfluidic perfusion platform for culturing zebrafish embryos and capturing live images of various tissues and organs inside the embryo. The Fish and Chips was micro-fabricated in silicon and glass for reproducibility and accuracy of the microfluidic structure. The microfluidic platform consists of three parts: a microfluidic gradient generator, a row of eight fish tanks, in which the fish embryos are individually placed, and eight output channels. The fluidic gradient generator supports dose-dependent drug and chemical studies. A unique perfusion system ensures a uniform and constant flow of media to the fish tank while the wastes are efficiently removed. The fish tanks restrict the embryo movements, except rotationally, for live imaging of internal tissues and organs. The embryos showed developmental abnormalities under the influence of the drug valproic acid (VPA).
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