期刊
LAB ON A CHIP
卷 12, 期 2, 页码 302-308出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1lc20777f
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资金
- Pioneer Research Center through National Research Foundation of Korea
- Ministry of Education, Science and Technology [2009-0082953]
- National Research Foundation Science (NRF) [2008-0062056]
- National Research Foundation of Korea [2008-0062056] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The in situ formation of membranes in microfluidic channels has been given attention because of their great potential in the separation of components, cell culture support for tissue engineering, and molecular transport for generation of chemical gradients. Among these, the porous membranes in microchannels are vigorously applied to generate stable chemical gradients for chemotaxis-dependent cell migration assays. Previous work on the in situ fabrication of membranes for generating the chemical gradient, however, has had several disadvantages, such as fluid leaking, uncontrollable membrane thickness, need of extra equipment, and difficulty in realizing stable interfacial layers. In this paper, we report a novel technique for the in situ formation of membranes within microchannels using enzymatically crosslinkable hydrogels and microfluidic techniques. The thickness of the membrane can be controlled quantitatively by adjusting the crosslinking reaction time and velocity of the microfluidics. By using these techniques, parallel dual hydrogel membranes were prepared within microchannels and these were used for the generation of stable concentration gradients. Moreover, the migration of Salmonella typhimurium was monitored to validate the efficacy of the chemical gradients. These results suggest that our in situ membrane system can be used as a simple platform to understand many cellular activities, including cell adhesion and migration directed by chemotaxis or complex diffusions from biological fluids in three-dimensional microstructures.
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