Article
Chemistry, Analytical
Chenying He, Yuanao Zhang, Ting Wen, Shenghai Pei, Zhixun Wang, Juan Xia, Guangcheng Xi, Wenjie Li, Jiahong Wang, Guoqiang Gu, Guohua Zhong, Lei Wei, Chunlei Yang, Ming Chen
Summary: A novel oxygen vacancy heteropolyacid (HFMO) is developed as a high-performance volume-enhanced Raman scattering (VERS)-active platform, which forms a special coordination bond with the probe molecule at the molecular level. This platform has excellent VERS enhancing effect and high selectivity for molecules containing the imino group. Rating: 9 out of 10.
ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Qishen Huang, Peter J. Vikesland
Summary: This study demonstrates the use of flash-freeze surface-enhanced Raman spectroscopy (SERS) to characterize the pH of micrometer-sized droplets generated from low solute concentration environmental water samples. The flash-freeze SERS approach preserves the initial droplet properties and provides greater sensitivity compared to pH paper. The results show that droplet and bulk pH were generally comparable for most environmental samples, except for one rainwater sample with a higher droplet pH. This indicates the potential influence of droplet composition on droplet pH.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Article
Engineering, Environmental
Yen-Hsiang Huang, Hong Wei, Peter J. Santiago, William John Thrift, Regina Ragan, Sunny Jiang
Summary: Rapid and cost-effective detection of antibiotics in wastewater and through wastewater treatment processes is crucial. Surface-enhanced Raman scattering (SERS) has the potential for label-free, real-time sensing of antibiotic contamination in the environment. This study tested two gold nanostructures as SERS substrates for the label-free detection of quinoline, a commonly found antibiotic in wastewater.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Yen-Hsiang Huang, Hong Wei, Peter J. Santiago, William John Thrift, Regina Ragan, Sunny Jiang
Summary: Rapid and cost-effective detection of antibiotics in wastewater is crucial for effective strategies in their removal. Surface-enhanced Raman scattering (SERS) can potentially provide label-free, real-time sensing of antibiotic contamination. This study tested two gold nanostructures as SERS substrates for the detection of quinoline, a commonly found antibiotic in wastewater. Results showed that the self-assembled SERS substrate had a low limit of detection (LoD) of 5.01 ppb for quinoline in wastewater. However, the commercially available SERS substrate performed poorly due to interference from non-target molecules.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Materials Science, Ceramics
Ton Nu Quynh Trang, Tieu Tu Doanh, Lam Quang Vinh, Vu Thi Hanh Thu
Summary: The study presents the design and fabrication of an Ag/TiO2 NRs heterostructure for ultrasensitive SERS detection of molecules like R6G and efficient degradation of molecules, showing promising photocatalytic performance and self-cleaning effect.
CERAMICS INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Seongyong Park, Abdul Wahab, Minseok Kim, Shujaat Khan
Summary: In this article, a self-supervised deep learning-based information fusion technique is proposed to minimize the variance in SERS measurements of the same target analyte across multiple laboratories. The proposed model, called MVNet, shows improved performance in predicting the concentration of unseen target analyte and minimizes the variance of completely unseen laboratory datasets.
Article
Nanoscience & Nanotechnology
Tieu Tu Doanh, Nguyen Van Hieu, Ton Nu Quynh Trang, Vu Thi Hanh Thu
Summary: Micro-structured molecular semiconductor film-based surface-enhanced Raman scattering (SERS) probes are important analytical tools for research. This study introduced a novel zinc oxide-based, three-dimensional semiconductor nanoflower superstructure probe with unique physicochemical properties, allowing effective molecular interactions between the ZnO platform and the Raman probe molecule. The synthesized ZnONFs@Ag chip demonstrated outstanding selectivity and reproducibility in quantitative and qualitative evaluation of target molecules at low concentrations.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2021)
Article
Biophysics
Lang Li, Yang Lu, Ziting Qian, Zhaoyan Yang, Kuo Yang, Shenfei Zong, Zhuyuan Wang, Yiping Cui
Summary: A microfluidic surface enhanced Raman spectroscopy (SERS) biosensor was developed for ultrasensitive and reliable detection of dopamine (DA) in serum, using DNA-assisted synthesis of ortho-nanodimers (DaSON) strategy. The SERS sensor demonstrated ultrahigh sensitivity and high accuracy, with a limit of detection as low as 10 aM in phosphate buffer saline and 10 fM in serum. Quantitative monitoring of extracellular DA released from living neurons and quantification of DA in human blood samples were successfully achieved, providing a promising tool for in-vitro investigation of neurological processes and clinical examination of dopaminergic disorders.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Shuo Zhu, Binge Deng, Fugang Liu, Jin Li, Li Lin, Jian Ye
Summary: Surface-enhanced Raman scattering (SERS) nanoprobes with high sensitivity and specificity of the fingerprint spectrum have limitations in signal-to-background ratio (SBR) and imaging requirements. Our developed bio-orthogonal nanoprobes overcome these limitations and achieve an ultrahigh SBR and ambient light anti-interference ability, opening up a new era for Raman imaging in clinical medicine.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Yanfang Cui, Jing Zheng, Wei Zhuang, Haiwang Wang
Summary: The novel SERS platform enables highly sensitive detection of miRNA-21 through target activation with exonuclease-assisted signal amplification, demonstrating a wide detection range and low detection limit. It is a promising tool for miRNA-21 SERS assay with great potential for cancer typing and clinical diagnostics.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Ting-tiao Pan, Wang Guo, Ping Lu, Deyu Hu
Summary: This study successfully established an in situ, nondestructive, and rapid SERS method for detecting acetamiprid residue on the surface of fruits and vegetables, which could serve as an auxiliary approach for early screening of contaminated produce in the field or on site in the future.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Chemistry, Analytical
Kohei Ide, Masahiro Kunimoto, Satoru Yoshida, Masahiro Yanagisawa, Takayuki Homma
Summary: An in situ surface-enhanced Raman spectroscopy (SERS) system was developed to detect pH changes near the electrode surface. By distributing probe molecules near the electrode, the system is able to detect and analyze pH changes through the corresponding Raman spectra.
Article
Multidisciplinary Sciences
Kandammathe Valiyaveedu Sreekanth, Jayakumar Perumal, U. S. Dinish, Patinharekandy Prabhathan, Yuanda Liu, Ranjan Singh, Malini Olivo, Jinghua Teng
Summary: The researchers present a scalable Tamm plasmon cavity using phase change material with large resonance tunability and demonstrated tunable SERS by matching the plasmonic resonance with the molecule absorption for sensitivity enhanced biosensing.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Huimin Zhao, Dawei Zheng, Huiqin Wang, Taifeng Lin, Wei Liu, Xiaoli Wang, Wenjing Lu, Mengjia Liu, Wenbo Liu, Yumiao Zhang, Mengdong Liu, Ping Zhang
Summary: This study aimed to develop a simple, sensitive, low-cost, environmentally friendly, and flexible surface-enhanced Raman scattering (SERS) platform for rapid and on-site detection of bacteria using a portable Raman spectrometer. The study demonstrated that the established SERS platform has practical applications in pathogenic bacterial identification and other rapid detections, providing advantages such as accurate discrimination, simple preparation, easy operation, good sensitivity, accuracy, and reproducibility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Moru Yang, Yuanyuan Pan, Chang Ji, Mingrui Shao, Zhen Li, Jing Yu, Chonghui Li, Baoyuan Man, Chao Zhang, Xiaofei Zhao
Summary: In this study, 3D MXene hollow spheres wrapped with silver nanoparticles were prepared using a sacrificial template method. The structure exhibited good light-trapping capability and localized surface plasmon resonance effect. It showed excellent sensitivity in SERS and catalytic performance, and could be used for in situ SERS monitoring of catalytic reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Materials Science, Multidisciplinary
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: This study proposes a novel bioprinting technique using a supportive microstructured bioprinting substrate to enhance the structural fidelity of low-viscosity hydrogel constructs and replicate the biological vessel formation process. This technology could be applied to developing complex constructs using cytocompatible natural hydrogels.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Chemistry, Multidisciplinary
Sumit Kumar, Mamata Karmacharya, Yoon-Kyoung Cho
Summary: Cells are the fundamental units of life and contain the necessary information for building living creatures. Advances in synthetic biology and cell engineering allow for the redesign of biological systems, but creating life-like structures from nonliving building blocks remains challenging. Cell membrane-engineered micro or nanoreactors have promising applications.
Article
Oncology
Jisu Lee, Hyun Sik Park, Seung Ro Han, Yun Hee Kang, Ji Young Mun, Dong Wook Shin, Hyun-Woo Oh, Yoon-Kyoung Cho, Myung-Shin Lee, Jinsung Park
Summary: Extracellular vesicles derived from urine, specifically urinary EVs (uEVs), have shown promise as diagnostic biomarkers for urogenital cancers. This study identified uEV-derived alpha-2-macroglobulin (a2M) as a novel diagnostic biomarker for bladder cancer (BC) and validated its upregulation in patient uEVs. The use of the centrifugal microfluidic tangential flow filtration device ExoDisc was found to be more effective for uEV protein analysis compared to classical differential centrifugation. These findings suggest that this approach for EV analysis can lead to the identification of clinically meaningful uEV-based biomarkers for cancer diagnosis.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biophysics
Jieun Han, Hye Jin Lim, Juhwan Park, Dong Hyun Han, Dong-Myung Kim, Je-Kyun Park
Summary: A dual sensing microfluidic device has been developed for fast, portable, and quantitative analysis of target amino acids, using the biological mechanism of protein synthesis. This device enables precise quantification of amino acids within 30 min at room temperature, indicating its potential for point-of-care testing of metabolic compounds.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki -Hun Jeong
Summary: This study presents a rapid and compact real-time RT-PCR system for decentralized molecular diagnostics. It utilizes plasmonic nucleic acid amplification and quantification, and features an ultrafast plasmonic thermocycler, a disposable plastic-on-metal cartridge, and a compact fluorescence microscope. The system successfully enables 10-minute diagnosis of COVID-19 with high amplification efficiency, classification accuracy, and total percent agreement for clinical diagnostic tests.
Article
Biophysics
Jonathan Sabate del Rio, Jooyoung Ro, Heejeong Yoon, Tae-Eun Park, Yoon-Kyoung Cho
Summary: Organs-on-chips (OoCs) are biomimetic in vitro systems that mimic the physicochemical microenvironments, physiologies, and key functional units of specific human organs using microfluidic cell cultures. These systems have the potential to replace animal models, enable personalized medicine, and require continuous monitoring of quality parameters. Integration of biosensing technologies into OoCs allows for monitoring of their physiologies, functions, and microenvironments. Future directions involve the application of artificial intelligence for process optimization, self-regulation, and data analysis in OoCs and cyber-physical systems.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Dong Hyun Han, Untaek Oh, Je-Kyun Park
Summary: Three-dimensional (3D) printing is a promising technology for fabricating microstructures, but its direct use as microchannels is limited. In this study, we replicated PDMS microchannels using 3D-printed molds and found that bonding two single PDMS layers significantly increased the optical transmittance. We evaluated the applicability of the replicated PDMS devices by comparing them to conventional Si-wafer molds and performed droplet generation in the PDMS microchannels. This study provides a fundamental understanding of prototyping microstructures from DLP-based 3D-printed molds.
Article
Engineering, Biomedical
Mamata Karmacharya, Sumit Kumar, Yoon-Kyoung Cho
Summary: Membrane fusion is an important phenomenon for cellular function. Extracellular vesicles (EVs) have the ability to transfer information between cells through fusion and can be used in diagnostics and therapeutics. This study explores the potential applications of EVs and their fusion with liposomes and cells, as well as methods to enhance the fusion process. EVs have high loading capacity, bio-compatibility, and stability, making them ideal for drug production and diagnostics. The unique properties of fused EVs and the necessary design and development procedures are also examined. The promise of EVs in disease management highlights their potential role in future healthcare.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: In this study, a novel approach for the hybrid biofabrication of complex and heterogeneous 3D constructs using low-viscosity bioinks is proposed. The approach involves the use of a micromesh substrate onto which PLA filament is extruded followed by the printing of fibrinogen. The micromesh supports the printed hydrogel with a capillary pinning effect, enabling high-resolution bioprinting. The 3D MOSAIC platform allows the fabrication of complicated and multimaterial 3D structures and the self-organization of cells within the hydrogel.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Gihyun Lee, Soo Jee Kim, Je-Kyun Park
Summary: The tumor microenvironment (TME) is crucial for the nourishment of tumors and delivery of chemotherapy drugs, but existing bioprinting and microfluidic technologies have not fully utilized the potential benefits of precise spatial control and adjustment of culture environments. This study introduces a novel integrative technology that combines a self-organized TME array bioprinted on a microfluidic chip with a vascular endothelial barrier surrounding breast cancer spheroids.
Article
Materials Science, Multidisciplinary
Sung-Pyo Yang, Jae-Myeong Kwon, Jae-Won Seo, Han Jo Jun, Kyungmin Hwang, Eun-Sun Kim, Ki-Hun Jeong
Summary: This study reports a method for in vivo 3D imaging of abnormal features during endoscopic operation using a light switching microprojector. The microprojector integrates rotational offset microlens arrays and a customized illumination fiber bundle through a single illumination channel of a clinical endoscope. The technique enables accurate measurement of the size and volume of abnormal tissues.
ADVANCED PHOTONICS RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Byoung-Hoon Kang, Kyung-Won Jang, Eun-Sil Yu, Hamin Na, Yun-Jae Lee, Woong-Yeol Ko, NamHo Bae, Donggee Rho, Ki-Hun Jeong
Summary: This study presents an ultrafast plasmonic nucleic acid amplification and real-time quantification system for decentralized molecular diagnostics. The system features ultrafast photothermal cycling, rapid heat transfer, and efficient PCR quantification, along with high-contrast fluorescence microscopic imaging. It enables decentralized point-of-care molecular diagnostic testing in primary care and developing countries.
Article
Nanoscience & Nanotechnology
Soo Jee Kim, Gihyun Lee, Je-Kyun Park
Summary: In this study, a novel approach for the hybrid biofabrication of complex and heterogeneous 3D constructs using low-viscosity bioinks was proposed. The microextrusion printing of fibrinogen onto PLA-framed micromesh substrates allowed for high-resolution bioprinting. The resulting 3D constructs could support the self-organization of endothelial cells and enable cancer cell migration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemical Research Methods
Junyoung Kim, Vijaya Sunkara, Jungmin Kim, Jooyoung Ro, Chi-Ju Kim, Elizabeth Maria Clarissa, Sung Wook Jung, Hee Jin Lee, Yoon-Kyoung Cho
Summary: Tumor-educated platelets play a critical role in tumorigenesis, cancer development, and metastasis. Researchers found that interleukin-8 (IL-8) in cancer-derived extracellular vesicles (EVs) contributed to platelet activation, resulting in increased platelet adhesion and potentially leading to tumor metastasis. Platelet adhesion levels can differentiate breast cancer patients with and without metastasis.
Review
Chemistry, Analytical
Jooyoung Ro, Junyoung Kim, Yoon-Kyoung Cho
Summary: Three-dimensional multicellular spheroid models can accurately recapitulate the tumor microenvironment and microfluidic platforms are extensively used to study spheroids and understand cellular interactions and discover cancer therapeutics.
