期刊
LAB ON A CHIP
卷 11, 期 6, 页码 1138-1143出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0lc00500b
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资金
- National Science Foundation
- United States Public Health Service (National Institute of Health)
- New York State Stem Cell Science (NYSTEM)
- NATIONAL CANCER INSTITUTE [R01CA152870, R01CA033505] Funding Source: NIH RePORTER
A microfabricated flow cytometer has been developed that is capable of detecting nearly all of the microparticles in an aqueous suspension. Current design allows for integrated coupling between an optical fiber-based detection system and the particle stream via hydrodynamic focusing. By adjusting the relative flow-rates at the auxiliary inputs of the focusing manifold, the particle stream can be steered out-of-plane relative to the illuminating laser, and similarly the particle stream can be squeezed or expanded. The microfabricated device was constructed in polydimethylsiloxane with cross-sectional microfluidic dimensions of 125 mu m x 125 mu m. Using the present device and method, fluorescent microparticles in aqueous solution were counted at an absolute counting efficiency of 91 +/- 4%. The coefficient of variation of the fluorescence pulse-heights for far-red fluorescent microparticles was 15%. The device exhibited a linear response to fluorescence intensity calibration microparticles as shown by comparison with a commercial cytometer instrument.
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