Article
Chemistry, Analytical
Honeyeh Matbaechi Ettehad, Christian Wenger
Summary: This study developed a miniaturized CMOS integrated silicon-based microfluidic system for characterizing and separating live and dead yeast cells using DEP technique. The system was validated for efficient, rapid and selective cell separation applications, laying the foundation for future commercial mass production. The study demonstrated the feasibility of CMOS embedded microfluidic for dielectrophoretic cell manipulation applications and its compatibility with CMOS production line and electronics.
Article
Multidisciplinary Sciences
Chenfei Hu, Shenghua He, Young Jae Lee, Yuchen He, Edward M. Kong, Hua Li, Mark A. Anastasio, Gabriel Popescu
Summary: The study introduces a method for assessing cell viability without the need for labeling, utilizing deep learning techniques combined with quantitative phase imaging to accurately determine cell survival status without causing damage to the cells.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Electrical & Electronic
Cesar Palacios Arias, Marc Jofre, Lluis Jofre, Jordi Romeu, Luis Jofre-Roca
Summary: The combination of microwave and microfluidic technologies allows for the efficient quantification of live/dead bioparticles. This study demonstrates the differentiation of single live/dead bioparticles using a microwave-microfluidic platform. The system utilizes a contactless current-sensing superheterodyne architecture with broadband frequency-operation bowtie electrodes and successfully measures the cytoplasmic content of live and dead-flowing bacteria.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Chemistry, Multidisciplinary
Felix Pfisterer, Neus Godino, Tobias Gerling, Michael Kirschbaum
Summary: In this study, a microfluidic flow protocol for selective electroporation of predefined target cells identified in real-time by high-quality microscopic image analysis was presented. This method allows selective poration of target cells while non-target cells remain unaffected in heterogeneous cell samples.
Article
Chemistry, Analytical
Adem Ozcelik, Sinan Gucluer, Tugce Keskin
Summary: This study presents a cost-effective method for continuous separation of live and dead cells using a 3D resin-printed microfluidic device. The experimental results demonstrate that the device successfully separates cells based on density differences, achieving separation efficiencies of over 95% and producing purer cell populations.
Article
Chemistry, Multidisciplinary
Jun-Fang Wang, Chun-Miao Zhao, Jian-Xing Yang, Xu He, Xiao-Liu Li, Jin-Mei Li, Ke-Rang Wang
Summary: A mannose-modified perylene monoimide derivative PMI-Man has been developed, which exhibits highly selective binding to double-stranded DNA molecules, potent live/dead cell imaging, and histological imaging via both confocal and light microscopies. This approach may be utilized to create a universal colorful staining method for human tissues suitable for both confocal and light microscopies.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Yi-Sin Chen, Charles Pin-Kuang Lai, Chihchen Chen, Gwo-Bin Lee
Summary: This study presents a novel method for label-free, contact-free isolation and recovery of extracellular vesicles (EVs) using optically-induced dielectrophoresis (ODEP) on a microfluidic platform, achieving rapid enrichment and automatic isolation and recovery of EVs with high efficiency.
Article
Biochemical Research Methods
Alexis Lefevre, Michael Gauthier, Pauline Bourgeois, Annie Frelet-Barrand, Aude Bolopion
Summary: This paper presents an experimental bench for studying the automatic trajectory control of T-lymphocytes using dielectrophoresis. The dielectrophoretic force is computed online based on a Fourier series model, and a controller based on visual feedback is used to control the cell's position in a microfluidic chip. Experiments show successful steering of T-lymphocytes along different trajectories at a speed of five times their size per second, with an average error below 2μm. Cell viability is confirmed after the experiments, indicating that this active DEP actuation does not harm the cells.
Article
Biochemistry & Molecular Biology
Vaidotas Stankevicius, Povilas Gibas, Bernadeta Masiulionyte, Liepa Gasiule, Viktoras Masevicius, Saulius Klimasauskas, Giedrius Vilkaitis
Summary: Enzymatic methylation of cytosine to 5-methylcytosine in DNA is a fundamental epigenetic mechanism involved in mammalian development and disease. In this study, the researchers engineered the Dnmt1 methyltransferase to catalyze the transfer of a synthetic cofactor analog onto DNA. By editing the Dnmt1 locus in mouse embryonic stem cells and using electroporation to introduce the cofactor, they were able to selectively tag Dnmt1-specific genomic targets. This new approach, called Dnmt-TOP-seq, allows for high-resolution tracking of Dnmt1 catalysis in mammalian cells and opens the door for selective studies of other methylation pathways in eukaryotic systems.
Article
Physics, Applied
Beth Ringwelski, Vidura Jayasooriya, Dharmakeerthi Nawarathna
Summary: CAR T-cell therapy is an FDA-approved treatment for specific cancers, but contamination of T-cells derived from patients with existing cancer cells during manufacturing may lead to significant adverse effects. Dielectrophoretic cell purification method showed high purity and viability in experiments, offering a solution to this issue.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2021)
Article
Chemistry, Analytical
Meltem Elitas, Monsur Islam, Jan G. Korvink, Esra Sengul, Pouya Sharbati, Beyzanur Ozogul, Sumeyra Vural Kaymaz
Summary: This study investigates the deformation and migration profile of glioma cells under the influence of dielectrophoretic forces, providing a rapid, robust, and sensitive method for quantifying membrane deformation of glioma cells to determine the state of the cells or efficacy of administrated drugs.
Article
Environmental Sciences
Xinxin Chen, Siheng Cai, Nan Zhang, Jieqiu Yang, Tangjian Peng, Fei Yang
Summary: Heavy metal pollution poses a serious threat to the environment and human health. Biosorption, a cost-effective and environmentally friendly method, has gained worldwide attention. This study investigates the biosorption of U(VI) onto live and dead cells of a Sphingopyxis strain YF1. The results show that both live and dead cells have good adsorption performance, with the maximal adsorption capacities of U(VI) being 140.7 mg g(-1) and 205.7 mg g(-1), respectively. The mechanisms of U(VI) adsorption by Sphingopyxis sp. YF1 were also revealed.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemical Research Methods
Kazuma Yoda, Yoshiyasu Ichikawa, Masahiro Motosuke
Summary: This paper presents a newly developed high-throughput measurement device for determining the dielectric properties of cancer cells. The device utilizes continuous-flow electrorotation to measure the rotational behavior of cells, resulting in a significant improvement in throughput compared to conventional devices.
Article
Energy & Fuels
Philipp Kunze, Johannes M. Greulich, Ammar Tummalieh, Wiebke Wirtz, Hannes Hoeffler, Nico Woehrle, Stefan Glunz, Stefan Rein, Matthias Demant
Summary: The current-voltage measurement is crucial in solar cell quality control. This study presents a contactless IV characterization method using an empirical model to derive full IV curves and parameters. Contactless measurements are based on photoluminescence, electroluminescence, and spectral reflectance data.
Article
Chemistry, Analytical
Kai Zhao, Penglu Zhao, Jianhong Dong, Yunman Wei, Bin Chen, Yanjuan Wang, Xinxiang Pan, Junsheng Wang
Summary: This study demonstrates the successful isolation of circulating tumor cells (CTCs) from blood cells using a combination of dielectrophoresis and magnetophoresis in a microfluidic chip. The results show the potential application of this method in diagnostics.
Article
Multidisciplinary Sciences
Michael B. Sano, Christopher B. Arena, Katelyn R. Bittleman, Matthew R. DeWitt, Hyung J. Cho, Christopher S. Szot, Dieter Saur, James M. Cissell, John Robertson, Yong W. Lee, Rafael V. Davalos
SCIENTIFIC REPORTS
(2015)
Article
Multidisciplinary Sciences
Jill W. Ivey, Eduardo L. Latouche, Michael B. Sano, John H. Rossmeisl, Rafael V. Davalos, Scott S. Verbridge
SCIENTIFIC REPORTS
(2015)
Article
Chemistry, Analytical
Erin A. Henslee, Ruth M. Torcal Serrano, Fatima H. Labeed, Rita I. Jabr, Christopher H. Fry, Michael P. Hughes, Kai F. Hoettges
Article
Multidisciplinary Sciences
Shabnam A. Faraghat, Kai F. Hoettges, Max K. Steinbach, Daan R. van der Veen, William J. Brackenbury, Erin A. Henslee, Fatima H. Labeed, Michael P. Hughes
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Erin A. Henslee, Priya Crosby, Stephen J. Kitcatt, Jack S. W. Parry, Andrea Bernardini, Rula G. Abdallat, Gabriella Braun, Henry O. Fatoyinbo, Esther J. Harrison, Rachel S. Edgar, Kai F. Hoettges, Akhilesh B. Reddy, Rita I. Jabr, Malcolm von Schantz, John S. O'Neill, Fatima H. Labeed
NATURE COMMUNICATIONS
(2017)
Article
Biochemical Research Methods
Roberto C. Gallo-Villanueva, Michael B. Sano, Blanca H. Lapizco-Encinas, Rafael V. Davalos
Article
Biology
Andrew D. Beale, Emily Kruchek, Stephen J. Kitcatt, Erin A. Henslee, Jack S. W. Parry, Gabriella Braun, Rita Jabr, Malcolm von Schantz, John S. O'Neill, Fatima H. Labeed
JOURNAL OF BIOLOGICAL RHYTHMS
(2019)
Article
Radiology, Nuclear Medicine & Medical Imaging
Jacob D. Kaufman, Christopher C. Fesmire, Ross A. Petrella, Callie A. Fogle, Lei Xing, David Gerber, Michael B. Sano
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY
(2020)
Article
Multidisciplinary Sciences
Kai F. Hoettges, Erin A. Henslee, Ruth M. Torcal Serrano, Rita Jabr, Rula G. Abdallat, Andrew D. Beale, Abdul Waheed, Patrizia Camelliti, Christopher H. Fry, Daan R. van der Veen, Fatima H. Labeed, Michael P. Hughes
SCIENTIFIC REPORTS
(2019)
Review
Biochemical Research Methods
Erin A. Henslee
Article
Multidisciplinary Sciences
Erin A. Henslee, Carina M. Dunlop, Christine M. de Mel, Emily A. Carter, Rula G. Abdallat, Patrizia Camelliti, Fatima H. Labeed
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Csaba Matta, Rebecca Lewis, Christopher Fellows, Gyula Diszhazi, Janos Almassy, Nicolai Miosge, James Dixon, Marcos C. Uribe, Sean May, Szilard Poliska, Richard Barrett-Jolley, Janos Fodor, Peter Szentesi, Tibor Hajdu, Aniko Keller-Pinter, Erin Henslee, Fatima H. Labeed, Michael P. Hughes, Ali Mobasheri
Summary: Chondrogenic progenitor cells (CPCs) may have superior chondrogenic potential compared to mesenchymal stem cells (MSCs), with a distinct channelome characteristic, including calcium-dependent potassium channels such as BK channels. The KCNMA1 gene was found to be upregulated in CPCs and associated with BK channel activity, impacting proliferation, gene expression, migration, and calcium events in CPCs. These findings contribute to a better understanding of CPCs and their potential in cartilage regeneration.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Michael Pycraft Hughes, Emily J. Kruchek, Andrew D. Beale, Stephen J. Kitcatt, Sara Qureshi, Zachary P. Trott, Oriane Charbonnel, Paul A. Agbaje, Erin A. Henslee, Robert A. Dorey, Rebecca Lewis, Fatima H. Labeed
Summary: The membrane potential V-m plays crucial roles in cell function, influencing extracellular potential and ion composition, which has significant implications for cellular interactions and disease development.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Capucine Martin, Jonathan D. Johnston, Erin A. Henslee, Daan R. van der Veen, Fatima H. Labeed
Summary: Research reveals that the electrical properties in adipocytes play a crucial role in peripheral clock regulation, independent of transcription-translation feedback loops. Using dielectrophoresis, significant electrophysiological rhythms were observed in pre-adipocytes, with circadian (around 24 hours) cycles in membrane capacitance and radius properties, and ultradian (around 14 hours) rhythms in membrane conductance.
Article
Engineering, Multidisciplinary
Christopher B. Arena, Paulo A. Garcia, Michael B. Sano, John D. Olson, Thomas Rogers-Cotrone, John H. Rossmeisl, Rafael V. Davalos