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Biology
Kazuhide S. Okuda, Mikaela Keyser, David B. Gurevich, Caterina Sturtzel, Elizabeth A. Mason, Scott Patterson, Huijun Chen, Mark Scott, Nicholas D. Condon, Paul Martin, Martin Distel, Benjamin M. Hogan
Summary: Angiogenesis is regulated by VEGF signaling pathways, with Erk playing a central role as a downstream effector. This study demonstrates the real-time imaging of Erk signaling dynamics in angiogenesis using a vascular Erk biosensor transgenic line in zebrafish. It reveals a dynamic sequence of Erk signaling events in regenerative angiogenesis, providing a new resource for studying vascular Erk signaling in real-time.
Article
Multidisciplinary Sciences
Jeremy Dufourt, Maelle Bellec, Antonio Trullo, Matthieu Dejean, Sylvain De Rossi, Cyril Favard, Mounia Lagha
Summary: By using the SunTag method, researchers visualized and quantified the translation of single mRNAs in living Drosophila embryos, revealing spatial heterogeneity in mRNA translation efficiency and the presence of translation factories. These findings open up new avenues for understanding the role of gene regulation during development in living multicellular organisms.
Article
Biology
Kwang Woo Ko, Laura Devault, Yo Sasaki, Jeffrey Milbrandt, Aaron DiAntonio
Summary: By using live imaging of mouse sensory neurons with single axon resolution, researchers discovered an ordered sequence of cellular events downstream of SARM1 activity, including loss of cellular ATP, defects in mitochondrial movement and depolarization, followed by calcium influx, externalization of phosphatidylserine, and loss of membrane permeability, leading to catastrophic axonal self-destruction.
Review
Biochemistry & Molecular Biology
Ji Min Shin, Ling Yuan, Tomokazu Kawashima
Summary: Seed development in flowering plants is a complex process regulated by genetically distinct tissues. Live-cell imaging techniques have allowed us to explore the dynamic events involved in seed development.
Article
Biology
Maria Podinovskaia, Cristina Prescianotto-Baschong, Dominik P. Buser, Anne Spang
Summary: This study introduces a novel live-cell imaging assay for monitoring endosome maturation process, applicable to various types of mammalian cells. It was found that different endosome conversion processes and acidification relied on different factors. This assay serves as a powerful tool to further unravel various aspects of endosome maturation.
Article
Biology
Yucen Hu, Jingxiu Xu, Erqing Gao, Xueyuan Fan, Jieli Wei, Bingcheng Ye, Suhong Xu, Weirui Ma
Summary: Imaging endogenous mRNAs in live animals is achieved using the MS2-based signal amplification with the Suntag system. This technique has high temporal resolution and utilizes 8xMS2 stem-loops, eliminating the need to insert a 1300 nt 24xMS2 into the genome for imaging endogenous mRNAs. By utilizing this tool, gene expression activation and dynamics of endogenous mRNAs in live C. elegans epidermis can be imaged.
Article
Astronomy & Astrophysics
M. M. Nyamai, L. Chomiuk, V. A. R. M. Ribeiro, P. A. Woudt, J. Strader, K. Sokolovsky
Summary: V445_Puppis is the only helium nova observed so far, with its eruption lasting nearly a decade and showing high velocities and a bipolar morphology. The study found that synchrotron emission dominates the radio light curve, and interactions between the wind and dense equatorial disc result in shocks and particle acceleration.
MONTHLY NOTICES OF THE ROYAL ASTRONOMICAL SOCIETY
(2021)
Article
Cell Biology
Hao Sun, Zhuo Zhang, Tianda Li, Ting Li, Weicai Chen, Tianshi Pan, Sen Fang, Chao Liu, Ying Zhang, Leyun Wang, Guihai Feng, Wei Li, Qi Zhou, Yuzheng Zhao
Summary: Metabolic programming is crucial in early embryonic development, and a noninvasive imaging technology is needed to track developmental metabolism. This study used genetically encoded fluorescent biosensors to characterize the regulation of energy metabolism and redox homeostasis during early zygotic cleavage. The results showed a decrease in NADH/NAD(+) levels and an increase in NADPH levels. Transcriptome profiling revealed changes in the expression of genes involved in glucose uptake, glycolysis, pyruvate metabolism, oxidative phosphorylation, and peroxiredoxin genes. Overall, this study provides insights into the programming of redox metabolism during ZGA.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ana Domingo-Muelas, Robin M. Skory, Adam A. Moverley, Goli Ardestani, Oz Pomp, Carmen Rubio, Piotr Tetlak, Blake Hernandez, Eric A. Rhon-Calderon, Luis Navarro-Sanchez, Carmen M. Garcia-Pascual, Stephanie Bissiere, Marisa S. Bartolomei, Denny Sakkas, Carlos Simon, Nicolas Plachta
Summary: Combining fluorescent dyes with live imaging, this study reveals the dynamics of early development in human embryos, including chromosome segregation, compaction, polarization, blastocyst formation, and hatching. The study also shows that blastocyst expansion mechanically affects trophectoderm cells, causing nuclear budding and DNA shedding into the cytoplasm. Moreover, the study suggests that aneuploidies in human embryos may not only result from chromosome segregation errors during mitosis, but also from nuclear DNA shedding.
Article
Cell Biology
Igor Kukhtevich, Mariana Rivero-Romano, Namisha Rakesh, Poonam Bheda, Yagya Chadha, Paulina Rosales-Becerra, Stephan Hamperl, Daniela Bureik, Scarlett Dornauer, Catherine Dargemont, Antonis Kirmizis, Kurt M. Schmoller, Robert Schneider
Summary: RNA distribution during cell division is asymmetric and regulated by factors such as the lifespan regulator Sir2 and replicative age. This study establishes an approach to quantify RNA dynamics in single cells using the diSpinach aptamer in a microfluidics device and validates it using budding yeast as a model.
Article
Biology
Peter Quicke, Yilin Sun, Mar Arias-Garcia, Melina Beykou, Corey D. Acker, Mustafa B. A. Djamgoz, Chris Bakal, Amanda J. Foust
Summary: Voltage imaging and machine learning techniques have revealed that human breast cancer cells exhibit voltage fluctuations which are not present in non-tumorigenic breast cells. This discovery sheds light on the bioelectric properties of cancer processes. Cancer cells have a depolarized resting membrane potential (V-m) and express active ionic conductances, which directly affect their pathophysiological behavior. By analyzing the V-m fluctuations using machine learning algorithms, researchers have identified four classes of breast cancer cells based on their Dynamic Electrical Signatures (DESs), ranging from noisy to blinking/waving.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yang Gao, Stefan Wennmalm, Bengt Winblad, Sophia Schedin-Weiss, Lars O. Tjernberg
Summary: Through FRET imaging, Aβ42 oligomerization was successfully detected in primary neurons, showing that Aβ42 oligomerized in lysosomes/late endosomes in a concentration-dependent manner. These findings contribute to a better understanding of Aβ42 oligomerization in neurons.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Daniel H. Goldman, Nathan M. Livingston, Jonathan Movsik, Bin Wu, Rachel Green
Summary: Translation of problematic mRNA sequences induces ribosome stalling, with slow ribosome rescue allowing cells to distinguish between transient and deleterious stalls. The dynamics of ribosome collisions and their recognition facilitate selective targeting for quality control.
Article
Biochemistry & Molecular Biology
Jaroslava Se Flova, Nima R. Habibi, John Q. Yap, Sean R. Cleary, Xuan Fang, Peter M. Kekenes-Huskey, L. Michel Espinoza-Fonseca, Julie B. Bossuyt, Seth L. Robia
Summary: The NKA-PLM regulatory complex consists of two alpha subunits associated with two beta subunits, decorated with two PLM regulatory subunits. This structure may have implications for the pathophysiology of heart failure.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Immunology
Nestor L. Uzcategui, Sena Gucer, Cris Richter, Annika Speidel, Elizabeta Zirdum, Michael Duszenko, Olga Garaschuk, Katherine Figarella
Summary: Our study revealed the early involvement of microglia in Trypanosoma brucei infection before invasion of the CNS. We found that microglia have an exceptional capability to inhibit parasite proliferation inside the brain in areas of initial homeostatic disruption near brain barriers. Additionally, early signs of inflammation in the meninges synchronized with the microglial response, along with a massive infiltration of peripheral immune cells into the parenchyma during the final disease stage.
FRONTIERS IN IMMUNOLOGY
(2023)