Article
Agriculture, Multidisciplinary
Pilaipan Chairuk, Raihan Uz Zaman, Jomkarn Naphatthalung, Chaweewan Jansakul
Summary: The study found that consumption of whole egg or egg yolk led to hypertension, with liver and kidney impairment following the intake of yolk alone. On the other hand, egg white was beneficial for the cardiovascular system as it decreased plasma cholesterol and body fat accumulation.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Medicine, General & Internal
Mi-Hyun Bae, Chan-Jeoung Park, Cheolwon Suh
Summary: MDSCs in patients with myeloma have prognostic significance, with their levels in both blood and bone marrow associated with disease progression. Patients with high levels of PB-mMDSCs have poorer prognosis. This study highlights the independent adverse prognostic impact of PB-mMDSCs in patients with myeloma.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Multidisciplinary Sciences
Arka Bhowmik, Biswajoy Ghosh, Mousumi Pal, Ranjan Rashmi Paul, Jyotirmoy Chatterjee, Suman Chakraborty
Summary: Precise information on localized variations in blood circulation is crucial for noninvasive cancer diagnostics and therapeutic assessment. We report a portable noninvasive blood perfusion imager augmented with machine learning-based quantitative analytics, which effectively screens precancerous and cancerous traits in oral lesions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Food Science & Technology
Gema Puertas, Manuel Vazquez
Summary: The study examined how operational conditions affect the composition and functional properties of plasma obtained by centrifugation, showing that plasma has lower protein content and dry matter than liquid whole egg. Under the conditions studied, plasma contained 74-85% of egg cholesterol, maintaining emulsifying activity and enhancing foaming properties. Plasma doubled foam capacity and increased foam stability by almost 15% compared to liquid whole egg, suggesting it could be a good substitute in the food industry.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Sien Ombelet, Alessandra Natale, Jean-Baptiste Ronat, Olivier Vandenberg, Jan Jacobs, Liselotte Hardy
Summary: The use of manual blood cultures without equipment is common in low-resource settings. However, the quality of manual blood culture bottles on the market is often unknown. To ensure acceptable quality, bottles can be evaluated using simulated blood cultures. A practical protocol for evaluating manual blood culture bottles in low-resource settings is proposed, including recommendations for blood types, micro-organism selection, and sample size.
Article
Biochemistry & Molecular Biology
Nadia Sitoe, Julia Sambo, Nedio Mabunda, Neuza Nguenha, Jorfelia Chilaule, Julio Rafael, Anesio Macicame, Imelda Chelene, Chishamiso Mudenyanga, Jillian Sacks, Sofia Viegas, Osvaldo Loquiha, Ilesh Jani
Summary: This study evaluated the performance of nasal swabs for SARS-CoV-2 detection in resource-poor settings and assessed the clinical performance of three nasal swab-based antigen rapid tests. The results showed that nasal swabs had slightly lower sensitivity compared to oropharyngeal swabs, and the COVIOS Ag-RDT did not meet the requirements set by the WHO. Nasal swab-based antigen rapid tests are a viable and accurate diagnostic option in resource-poor settings.
Article
Multidisciplinary Sciences
Jordan Wood, Larry J. Minter, Doug Bibus, Michael K. Stoskopf, Vivek Fellner, Kimberly Ange-van Heugten
Summary: This study compared different blood sampling methods for evaluating circulating fatty acid composition in African savannah elephants, finding that the fatty acid profiles of serum, plasma, whole blood, and DBS were comparable in these animals. The DBS method offers advantages in acquisition and handling, making it preferable for both routine health assessment of captive animals and field research on free-ranging animals.
Article
Clinical Neurology
Yvonne A. Eiby, Barbara E. Lingwood, Ian M. R. Wrightt
Summary: Preterm infants are at high risk of death and disability due to brain injury caused by impaired cardiovascular function. Research indicates that loss of plasma from circulation leading to hypovolemia is a major driver of cardiovascular instability and poor cerebral oxygenation in preterm infants. Changes in capillary permeability, pressure gradients, and lymphatic return contribute to increased plasma loss at the capillary level.
FRONTIERS IN NEUROLOGY
(2021)
Article
Biochemical Research Methods
Xiaoguang Lu, Mahnoush Tayebi, Ye Ai
Summary: The study developed an inertial microfluidics-based cell sorter for high-throughput isolation and enrichment of WBCs, providing an efficient and effective platform for WBC separation. The sorter is designed to enable broad application for size-based inertial cell sorting and can further enrich specific cell types using different microchips with varying cutoff sizes.
Article
Genetics & Heredity
Sung Hye Kim, David A. MacIntyre, Lynne Sykes, Maria Arianoglou, Phillip R. Bennett, Vasso Terzidou
Summary: This study examined the effect of whole blood holding time on the stability of plasma miRNA expression profiles. The results demonstrated that holding time can have dramatic effects on analytical reliability and reproducibility.
FRONTIERS IN GENETICS
(2022)
Article
Medicine, General & Internal
Sien Ombelet, Alessandra Natale, Jean-Baptiste Ronat, Olivier Vandenberg, Liselotte Hardy, Jan Jacobs
Summary: The MicroScan identification panels showed excellent accuracy for Gram-negative species and good accuracy for Gram-positive species in low-resource settings. Improvements are needed in stability, robustness, and ease of use to adapt to constraints in low-resource settings.
Article
Chemistry, Analytical
Somayyeh Bakhtiaridoost, Hamidreza Habibiyan, Hassan Ghafoorifard
Summary: This study presents a fast, hemolysis-free, highly efficient blood plasma separation microfluidic device that combines gravitational sedimentation with dielectrophoresis force. Using finite element analysis, the effect of geometrical parameters on the separation process is investigated to achieve optimal design specifications. The device achieves a purity of 99.98% and a relative increase in flow rate compared to previous studies by utilizing a porous Melt-Blown Polypropylene layer and optimizing the dielectrophoresis force.
ANALYTICA CHIMICA ACTA
(2023)
Article
Public, Environmental & Occupational Health
Golden Apuleni, Choolwe Jacobs, Patrick Musonda
Summary: The study indicated that health care seeking behaviors for common childhood illnesses in poor resource settings in Zambia were relatively high and could be predicted by mother's education level and marital status. Integrating interventions focused on increasing maternal and child health service utilization with basic education for women and support counseling for families may help maximize health seeking behaviors in marginalized communities.
FRONTIERS IN PUBLIC HEALTH
(2021)
Article
Chemistry, Analytical
Farshad Ardakani, Bahram Hemmateenejad
Summary: In this study, a new type of microfluidic paper-based analytical device (μPAD) was developed for the separation of plasma from whole blood without using plasma separation membrane. By controlling the pore size of the paper through lamination and electrolyte addition, the device achieved a longer separation distance for plasma with no significant hemolysis. The device has the potential for multi-assay testing in point-of-care experiments.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Gayan C. Bandara, Linus J. Unitan, Matthew H. Kremer, Owen T. Shellhammer, Shay Bracha, Vincent T. Remcho
Summary: Traditional blood separation methods require complex equipment and large sample volumes, but with the development of microfluidic devices, blood separation and analysis can now be achieved effectively in small sample sizes. By utilizing a simple fabrication process, these devices can separate blood and integrate colorimetric assay chemistries for semi-quantitative detection in microscale samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Hematology
G. Khanal, R. A. Huynh, K. Torabian, H. Xia, E. Voros, S. S. Shevkoplyas
Article
Hematology
Eszter Voros, Nathaniel Z. Piety, Briony C. Strachan, Madeleine Lu, Sergey S. Shevkoplyas
AMERICAN JOURNAL OF HEMATOLOGY
(2018)
Article
Cell & Tissue Engineering
Briony C. Strachan, Hui Xia, Eszter Voros, Sean C. Gifford, Sergey S. Shevkoplyas
Review
Medicine, Research & Experimental
Madeleine Lu, Minke A. E. Rab, Sergey S. Shevkoplyas, Vivien A. Sheehan
EXPERIMENTAL BIOLOGY AND MEDICINE
(2020)
Article
Hematology
Jonathan Robidoux, Audrey Laforce-Lavoie, Steve J. Charette, Sergey S. Shevkoplyas, Tatsuro Yoshida, Danny Brouard, Antoine Lewin
Article
Hematology
Madeleine Lu, Sergey S. Shevkoplyas
Article
Physiology
Madeleine Lu, Celeste K. Kanne, Riley C. Reddington, Dalia L. Lezzar, Vivien A. Sheehan, Sergey S. Shevkoplyas
Summary: The study introduces a microfluidic device capable of evaluating red blood cell deformability and adhesiveness, demonstrating slower perfusion rates for sickle red blood cells compared to normal red blood cells in LN-coated networks. This indicates the potential utility of the device for assessing novel therapies in SCD patients.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Christian A. Boada, Assaf Zinger, Scott Rohen, Jonathan O. Martinez, Michael Evangelopoulos, Roberto Molinaro, Madeleine Lu, Ramiro Alejandro Villarreal-Leal, Federica Giordano, Manuela Sushnitha, Enrica De Rosa, Jens B. Simonsen, Sergey Shevkoplyas, Francesca Taraballi, Ennio Tasciotti
Summary: Apolipoprotein-based drug delivery using lipid-based nanoparticles called Aposomes shows promising potential for targeted drug delivery. These Aposomes, with native apoB-100 protein on the surface, demonstrated active drug loading capabilities and enhanced targeting towards atherosclerotic plaques in a mouse model. The study highlights the feasibility of utilizing apolipoprotein-based approaches to create nanoparticles with active targeting capabilities for potential cardiovascular therapies.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Dalia L. Lezzar, Fong W. Lam, Ravin Huerta, Anton Mukhamedshin, Madeleine Lu, Sergey S. Shevkoplyas
Summary: This study introduces a novel high-throughput microfluidic device based on controlled incremental filtration technology for efficient leukapheresis. Compared to traditional centrifugation-based separation, the device has a smaller void volume, faster separation speed, and fewer platelet losses, with no measurable damage to red blood cells. The study demonstrates for the first time the highly efficient separation of cells from recirculating blood using a microfluidic device, indicating the feasibility of centrifugation-free, low-ECV leukapheresis using high-throughput microfluidic cell separation technology. This capability would be particularly useful for underserved young children.
SCIENTIFIC REPORTS
(2022)
Article
Hematology
Matthew S. Karafin, Joshua J. Field, Anton Ilich, Lang Li, Bahjat F. Qaquish, Sergey S. Shevkoplyas, Tatsuro Yoshida
Summary: This study found that plasma from SCD patients during crisis may affect the functionality of donor red blood cells, while hypoxic storage can preserve their deformability and reduce hemolysis. This has important implications for improving transfusion efficacy.
Correction
Multidisciplinary Sciences
Hui Xia, Briony C. Strachan, Sean C. Gifford, Sergey S. Shevkoplyas
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Kumar Abhishek, Anto Sam Crosslee Louis Sam Titus, Mai T. P. Dinh, Anton Mukhamedshin, Chandra Mohan, Sean C. Gifford, Sergey S. Shevkoplyas
Summary: This study describes a new approach that enables the size-based separation of desired lymphocyte subsets by artificially enlarging unwanted lymphocytes and other blood cells. The new rosette-enabled size-based (RESIZE) separation approach is faster and more efficient than the standard immunodensity method, with similar recovery rates but higher purity for lymphocyte subsets. The integration of RESIZE into the manufacturing workflow of cellular immunotherapies could greatly streamline the process and improve their cost and availability.
Review
Immunology
Kumar Abhishek, Malavika Nidhi, Srinandhini Chandran, Sergey S. Shevkoplyas, Chandra Mohan
Summary: Regulatory T cells (Tregs) are a type of lymphocyte that regulate the immune system by suppressing unwanted immune responses, preventing autoimmune diseases and inappropriate inflammatory reactions. They have shown promise in preventing graft vs. host disease, alleviating autoimmune symptoms, and promoting transplant tolerance. This review provides an overview of Treg cells, including important markers and subsets, as well as the methodology used for manufacturing adoptive regulatory T cell therapies (TRACT). The approaches and outcomes of several clinical trials involving adoptive transfer of Tregs to patients are also discussed.
CLINICAL IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Austin H. Routt, Natalia Yang, Nathaniel Z. Piety, Madeleine Lu, Sergey S. Shevkoplyas
Summary: We developed a highly accurate open-source method for classifying the morphology of red blood cells (RBCs) based on deep learning. The method allows for easy use in research and clinical applications and revealed insights into the degradation of RBC functional properties during hypothermic storage.
SCIENTIFIC REPORTS
(2023)
Review
Hematology
Madeleine Lu, Dalia L. Lezzar, Eszter Voros, Sergey S. Shevkoplyas
JOURNAL OF BLOOD MEDICINE
(2019)