Article
Biochemical Research Methods
Yi-Da Chung, Ting-Hang Liu, Yu-Ling Liang, Chang-Ni Lin, Keng-Fu Hsu, Gwo-Bin Lee
Summary: An integrated microfluidic system was developed for detecting the ovarian clear cell carcinoma biomarker FXYD2 automatically. This system could perform a series of operations from handling ascites to detecting RNA levels on a single microfluidic chip, offering potential for OCCC diagnosis and prognosis.
Article
Chemistry, Analytical
Najeeb Ullah, Wei Chen, Beenish Noureen, Yulan Tian, Liping Du, Chunsheng Wu, Jie Ma
Summary: An EIS sensor based on MXene nanosheets was developed for STX detection, achieving high sensitivity and good specificity through aptamer modification, with a low detection limit of 0.03 nM. The method demonstrated good selectivity and stability, showing potential for detecting STX in real samples.
Article
Chemistry, Multidisciplinary
Jing Wang, Yung-Ching Kao, Quan Zhou, Alain Wuethrich, Mitchell S. Stark, Helmut Schaider, H. Peter Soyer, Lynlee L. Lin, Matt Trau
Summary: Sensitive nanotechnology is used to profile serum extracellular vesicles for early melanoma detection, and the combination of microfluidic device and SERS technology can accurately differentiate melanoma patients from healthy individuals.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Physical
Quan Liu, Guodong Yu, Chongyu Zhu, Bo Peng, Ruohan Li, Tao Yi, Yanlei Yu
Summary: By integrating Laplace pressure and capillary condensation in a photodeformable microfluidic platform, various liquid operations such as propulsion, fusion, separation, and mixing of liquid slugs can be achieved without the need for microvalves. This technology has potential applications in miniaturized biomedical fields like portable analysis systems.
Article
Chemistry, Analytical
Yan Jin, Jingyi Wang, Zhiqiang Wang, Peng Xiong, Jianing Cheng, Tongyu Xu
Summary: This paper presents an integrated microfluidic biosensing system for rapid and sensitive detection of Salmonella typhimurium. The system consists of a microfluidic chip with a versatile valve, recombinase polymerase amplification (RPA) for nucleic acid detection, and a customized real-time fluorescence detection system. The system exhibits the potential to detect Salmonella at concentrations as low as 1.0 x 10(2) copies/mu L within 30 min, consistent with real-time quantitative polymerase chain reaction (qPCR) tests. The versatile valve in this integrated microfluidic biosensing system holds significant potential for on-site detection of foodborne pathogens.
Article
Chemistry, Multidisciplinary
Ge Li, Hao Li, Jiang Zhai, Jiazhuang Guo, Qing Li, Cai-Feng Wang, Su Chen
Summary: A visual microfluidic detection platform using CdTe quantum dots was developed for rapid and sensitive detection of veterinary drugs. The CdTe QDs showed selective fluorescence response to specific veterinary drugs, with different ligands displaying varying degrees of response. The platform offers simple operation, low cost, rapid sensing, and good sensitivity for quantitative detection of veterinary drug residues.
Article
Chemistry, Analytical
Zheng Li, Liyan Hua, Liming Xie, Dou Wang, Xingyu Jiang
Summary: There is an urgent need for point-of-care testing (POCT) devices that can integrate sample pretreatment and nucleic acid detection rapidly, economically, and without requiring extensive labor. In this study, we have developed an automated, portable nucleic acid detection system that combines microfluidic chips with rotary valve-assisted sample pretreatment and recombinase polymerase amplification (RPA)-T7-Cas13a for one-step nucleic acid detection. Our method achieved a detection sensitivity of 8 copies/reaction for Group B streptococci (GBS) DNA, which is 6 times more sensitive than gold-standard polymerase chain reactions (PCRs). The integration of RPA and clustered regularly interspaced short palindromic repeats (CRISPR)/Cas13a ensures ultra-specificity, accurately detecting GBS from 8 different types of pathogenic bacteria. The assay achieved 100% accuracy compared to PCR for 16 positive and 24 negative clinical GBS samples, with the entire procedure completed automatically within 30 minutes, providing a robust, sensitive, and accurate molecular diagnostic tool for POCT.
ANALYTICAL CHEMISTRY
(2023)
Article
Fisheries
Thanwarat Sukonta, Saengchan Senapin, Suwimon Taengphu, Pimkhuan Hannanta-anan, Mintra Kitthamarat, Praphutson Aiamsa-at, Thawatchai Chaijarasphong
Summary: Researchers have developed a novel method for detecting Tilapia lake virus (TiLV) using CRISPR-Cas12a technology and reverse-transcriptase recombinase polymerase amplification (RT-RPA-Cas12a), which is rapid, accurate, and user-friendly.
Article
Chemistry, Analytical
Shihua Luo, Yuan Wu, Weilun Pan, Guangzhi Zhong, Bo Situ, Bo Li, Xinyi Ye, Xiujuan Jiang, Wenbin Li, Ye Zhang, Lei Zheng, Qian Wang
Summary: In this study, a new platform using an integrated magneto-fluorescent exosome (iMFEX) nanosensor was developed for rapid and sensitive detection of cancer-derived exosomes. The iMFEX nanosensor exhibited excellent specificity and sensitivity and showed potential for clinical application.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Hua Wang, Jingsong Xu, Shun Li, Xi Wang, Gang Liu, Shuang Yang, Fei Zhao, Qian Liu, Xiang Chen, Chao He, Min Li
Summary: This study presents an integrated dual-layer microfluidic platform for simultaneous detection of SARS-CoV-2, influenza viruses A (FluA) H1N1, H3N2, and influenza virus B (FluB). The platform includes a dual microchip and a portable detection device for real-time fluorescence detection, temperature control and online communication. The assay showed high agreement with RT-PCR-based assays and has the potential to provide rapid screening of multiple viruses in various healthcare settings.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Alexandra Perebikovsky, Yujia Liu, Alexander Hwu, Horacio Kido, Ehsan Shamloo, Dian Song, Gabriel Monti, Oren Shoval, Daniel Gussin, Marc Madou
Summary: This study demonstrates a novel sample incubation technique on a microfluidic centrifugal disc, showing enhanced incubation for E. coli isolates and successful identification of antibiotic resistance within under 2 hours.
Article
Engineering, Electrical & Electronic
Goran M. Stojanovic, Tijana Kojic, Mitar Simic, Aleksandra Jovanovic-Galovic, Bojan Pavlovic, Amaia Zurutuza, Luca Anzi, Roman Sordan
Summary: A compact microfluidic platform for selective detection of ascorbic acid was presented in this paper, with the application of graphene improving response linearity and reducing detection and quantification limits compared to the reference structure.
IEEE SENSORS JOURNAL
(2021)
Article
Materials Science, Multidisciplinary
Adrian T. Nash, Daniel A. N. Foster, Shyan Thompson, Seungyoon Han, Madison K. Fernandez, Dae Kun Hwang
Summary: Hydrogel-based biosensing has been widely used in biomedical applications, but the long detection time and the need for blood sample prefiltration are limitations. In this study, a rapid microfluidic platform with cross-flow-induced advective transportation and size-based filtration is introduced, significantly reducing the detection time to under 15 minutes and eliminating the need for blood sample prefiltration.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
You-Ru Jhou, Chih-Hung Wang, Huey-Pin Tsai, Yan-Shen Shan, Gwo-Bin Lee
Summary: An integrated microfluidic platform utilizing real-time reverse-transcription loop-mediated isothermal amplification was developed for detection and quantification of three genes of SARS-CoV-2, providing a useful tool for COVID-19 diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Xiaoyu Zhao, Xiang Li, Weihao Yang, Jiwei Peng, Jiajun Huang, Shengli Mi
Summary: The study developed a microfluidic detection system for diagnosing high-risk HPV with good detection performance. The system integrates nucleic acid purification, isothermal amplification, and real-time fluorescence detection, offering automated and rapid diagnosis with broad potential for resource-limited settings.
Article
Biochemical Research Methods
C. S. Ball, R. F. Renzi, A. Priye, R. J. Meagher
Article
Multidisciplinary Sciences
Zachary W. Bent, Kunal Poorey, Annette E. LaBauve, Rachelle Hamblin, Kelly P. Williams, Robert J. Meagher
Editorial Material
Biochemistry & Molecular Biology
Robert J. Meagher, Oscar A. Negrete, Koen K. Van Rompay
TRENDS IN MOLECULAR MEDICINE
(2016)
Article
Chemistry, Analytical
Robert J. Meagher, Aashish Priye, Yooli K. Light, Cheng Huang, Eryu Wang
Article
Dentistry, Oral Surgery & Medicine
R. J. Meagher, E. Kousvelari
Article
Multidisciplinary Sciences
Sarah S. Wheeler, Cameron S. Ball, Stanley A. Langevin, Ying Fang, Lark L. Coffey, Robert J. Meagher
Article
Multidisciplinary Sciences
Minsoung Rhee, Yooli K. Light, Robert J. Meagher, Anup K. Singh
Article
Multidisciplinary Sciences
Aashish Priye, Sara W. Bird, Yooli K. Light, Cameron S. Ball, Oscar A. Negrete, Robert J. Meagher
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Analytical
Aashish Priye, Cameron S. Ball, Robert J. Meagher
ANALYTICAL CHEMISTRY
(2018)
Article
Immunology
Rudy Jacquet, Annette E. LaBauve, Lavoisier Akoolo, Shivani Patel, Abdulelah A. Alqarzaee, Tania Wong Fok Lung, Kunal Poorey, Timothy P. Stinear, Vinai C. Thomas, Robert J. Meagher, Dane Parker
INFECTION AND IMMUNITY
(2019)
Review
Pathology
Taylor J. Moehling, Gihoon Choi, Lawrence C. Dugan, Marc Salit, Robert J. Meagher
Summary: LAMP technology has played a significant role in molecular diagnostics over the past decade, particularly in field-deployable applications in resource-limited regions. This review outlines front-end LAMP design practices for point-of-care applications and discusses challenges and opportunities for future development of LAMP assays. Despite its popularity, there are still areas for improvement in the fundamental understanding of LAMP to elevate the field of assay development and characterization.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2021)
Article
Biotechnology & Applied Microbiology
Nileena Velappan, Hau B. Nguyen, Sofiya Micheva-Viteva, Daniel Bedinger, Chunyan Ye, Betty Mangadu, Austin J. Watts, Robert Meagher, Steven Bradfute, Bin Hu, Geoffrey S. Waldo, Antonietta M. Lillo
Summary: In this study, 18 unique anti SARS-CoV-2 human single-chain antibodies were isolated from an antibody library derived from healthy donors. The antibodies were characterized for their specificity, affinity, epitope diversity, and ability to neutralize SARS-CoV-2 infection. The results showed that these antibodies have high potential for therapeutic and diagnostic applications, offering a widely accessible and low-cost alternative to more sophisticated and expensive antibody selection approaches.
Review
Pathology
Gihoon Choi, Taylor J. Moehling, Robert J. Meagher
Summary: The SARS-CoV-2 pandemic has increased the use of RT-LAMP in research and development due to limitations in standard diagnostics. RT-LAMP has also made its way into the commercial market with emergency use authorizations for clinical diagnosis.
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2023)
Article
Chemistry, Analytical
Christopher R. Phaneuf, Kyle J. Seamon, Tyler P. Eckles, Anchal Sinha, Joseph S. Schoeniger, Brooke Harmon, Robert J. Meagher, Vinay V. Abhyankar, Chung-Yan Koh
ANALYTICAL METHODS
(2019)
Article
Chemistry, Analytical
C. Eid, S. S. Branda, R. J. Meagher