Article
Biochemical Research Methods
Runxuan Zhang, Tao Liao, Xiao Wang, Hong Zhai, Di Yang, Xin Wang, Haiyan Wang, Feng Feng
Summary: This study reports an immunochromatographic strip technology using polystyrene nanoparticles and near-infrared fluorescent dye for rapid detection of influenza virus. The method demonstrates excellent specificity and sensitivity, with stable performance under storage conditions.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Ruoxu Li, Ning Su, Xiaodong Ren, Xiange Sun, Wenman Li, Yuwei Li, Jin Li, Chen Chen, Hong Wang, Weiping Lu, Shaoli Deng, Qing Huang
Summary: This study developed a centrifugal microfluidics-based multiplex RT-RPA assay for rapid detection of SARS-CoV-2. The microfluidic chip could simultaneously detect three target genes and one reference gene, with high sensitivity and specificity. It has the potential to be used for point-of-care testing and detection of emerging SARS-CoV-2 variants.
Article
Virology
Xu Zhou, Siwen Wang, Yue Ma, Yanbing Li, Guohua Deng, Jianzhong Shi, Xiurong Wang
Summary: A reverse transcription recombinase polymerase amplification (RT-RPA)/CRISPR method was developed for rapid and sensitive detection of avian influenza virus (AIV). The new system has the potential to be an accurate, user-friendly, and inexpensive platform for point-of-care testing applications.
Article
Biophysics
Derrick Butler, Aida Ebrahimi
Summary: This paper presents a novel biosensor design for rapid, low-cost, and sensitive virus detection using a generator-collector microelectrode design. The effects of experimental and geometric factors on the sensor design are studied, and two different configurations are explored. The results show that the proposed design has higher electrochemical current and faster virus capture time, making it suitable for rapid detection and low viral loads.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yang Bu, Sheng Ni, Levent Yobas
Summary: This article investigates the mechanism of synchronous coefficient of drag alteration and finds that using a microfabricated artificial sieve can reduce the purification time of DNA from hours to minutes. An electrophoretic protocol is also proposed to facilitate the collection of purified DNA from the sieve, which can be used for rapid diagnostic applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Vishakha Nirbhaya, Chhaya Chaudhary, Dipti Chauhan, Ramesh Chandra, Suveen Kumar
Summary: In this study, a highly efficient biosensing platform for the detection of swine flu was fabricated using PIN/MTs nanohybrid material and electrophoretic deposition technique. Structural and morphological studies revealed good sensitivity and quick analysis time of the fabricated immunoelectrode. Additionally, the electrochemical response of the biosensor was validated using spiked artificial serum samples.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Virology
Guosong Wang, Pengfei Huang, Junping Hong, Rao Fu, Qian Wu, Ruiqi Chen, Lina Lin, Qiangyuan Han, Honglin Chen, Yixin Chen, Ningshao Xia
Summary: The ELISPOT assay based on the efficient detection of influenza virus and neutralizing antibodies is rapid, accurate, and high-throughput, serving as a powerful tool for quantification in the research and development of drugs and vaccines against influenza. The study demonstrated a good correlation with traditional PFU and PRNT methods, making it a valuable alternative in influenza studies.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Alvaro Lopez-Valinas, Marta Valle, Miaomiao Wang, Ayub Darji, Guillermo Cantero, Chiara Chiapponi, Joaquim Segales, Llilianne Ganges, Jose I. Nunez
Summary: Influenza A viruses have the ability to infect a wide range of bird and mammal species. They constantly evolve due to low proofreading activity and genomic reassortment, posing a threat to human and animal health. This study found that vaccination can reduce the likelihood of genomic reassortment and may influence the evolution of swine influenza viruses.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Dongjie Chen, Di Wang, Caixia Wang, Fang Wei, Hongyuan Zhao, Xiangmei Lin, Shaoqiang Wu
Summary: The study developed a rapid and highly sensitive ASFV antigen detection method based on AlphaLISA, which showed high specificity and outperformed other detection methods in porcine serum samples. The method serves as a sensitive and efficient laboratory diagnostic tool for ASFV detection.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Siwenjie Qian, Yanju Chen, Cheng Peng, Xiaofu Wang, Hui Wu, Yang Che, Huanying Wang, Junfeng Xu, Jian Wu
Summary: This article introduces a simple and cost-effective method for detecting African swine fever virus (ASFV). The method utilizes filter paper to purify nucleic acids from swine blood and employs CRISPR/Cas12a-mediated loop-mediated isothermal amplification (LAMP) reaction to detect ASFV. The method does not require complex laboratory conditions, and has the advantages of rapid and visualized detection, making it suitable for on-site application.
Article
Immunology
Songqi Wang, Haiyan Shen, Qijie Lin, Jun Huang, Chunhong Zhang, Zhicheng Liu, Minhua Sun, Jianfeng Zhang, Ming Liao, Yugu Li, Jianmin Zhang
Summary: In this study, a new detection method for African swine fever virus (ASFV) was established using cleaved probe-based loop-mediated isothermal amplification (CP-LAMP). The method achieved specific identification and visual detection of ASFV with high sensitivity and rapidity. By combining smartphone quantitation and a self-designed 3D-printed visualization function cassette, the CP-LAMP assay became a user-friendly, cost-effective, portable, rapid, and accurate point-of-care testing (POCT) platform for ASFV.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Kathrin Lillie-Jaschniski, Marina Lisgara, Emanuela Pileri, Agnes Jardin, Eduardo Velazquez, Monika Kochling, Michael Albin, Carlos Casanovas, Vassilis Skampardonis, Julia Stadler
Summary: The study evaluated a standardized sampling procedure for detecting influenza A virus in epidemically and endemically infected farms. Sampling different age groups is essential to obtain a comprehensive overview, as different subtypes were found in different age groups. Sampling strategies should also aim to include non-diseased animals.
VETERINARY SCIENCES
(2022)
Article
Immunology
Jamil N. Kanji, Kanti Pabbaraju, Matthew Croxen, Susan Detmer, Nathalie Bastien, Yan Li, Anna Majer, Hussein Keshwani, Nathan Zelyas, Ifeoma Achebe, Corinne Jones, Maureen Rutz, Angela Jacobs, Keith Lehman, Deena Hinshaw, Graham Tipples
Summary: A variant influenza A subtype H1N2 of swine origin was detected in a person in Alberta, Canada, and was found to be closely related to strains at a local pig farm. Quick collaboration between human and veterinary public health practitioners enabled a rapid response and surveillance showed no secondary cases in the surrounding area.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Chemistry, Analytical
Chengfei Li, Zhong Zou, Changjie Lv, Ya Zhao, Pengfei Han, Xiaomei Sun, Meilin Jin
Summary: In this study, a reliable and sensitive suspension microarray technology was developed for ASFV antibody detection. The newly developed method showed a 16-fold improvement in detection sensitivity compared to commercial ELISA kits and demonstrated a 97% consistency rate. This method has great potential for ASF epidemic surveillance and control.
ANALYTICA CHIMICA ACTA
(2022)