Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Santiago Rodriguez, Aina Garcia-Garcia, Eduardo Garcia-Calvo, Vanesa Esteban, Carlos Pastor-Vargas, Araceli Diaz-Perales, Teresa Garcia, Rosario Martin
Summary: This study describes the use of phage display technology to generate a recombinant antibody for specific detection of hen's ovomucoid, addressing the need for reliable egg allergen detection methods. The developed phage enzyme-linked immunosorbent assay (ELISA) demonstrated good sensitivity and accuracy, and showed consistent results with a commercial egg detection assay in the analysis of various food products. In silico modeling of the antigen-antibody complex provided insights into the interaction mechanism.
Article
Chemistry, Analytical
Ana Castanheira, Marilia Barreiros dos Santos, Laura Rodriguez-Lorenzo, Raquel Queiros, Begona Espina
Summary: This study developed a microfluidic system for detecting OA with high sensitivity and a wide linear range, capable of detecting low concentrations of OA in contaminated mussels; the enzyme stability and reusability, as well as the simplicity and low cost of microfluidic systems, make this method commercially attractive.
Article
Food Science & Technology
Mengzhen Hao, Shuai Yang, Shiwen Han, Huilian Che
Summary: Quail egg ovomucoid and hen egg ovomucoid have differences in structure and function, and quail egg ovomucoid has potential anti-allergic effects.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Biochemistry & Molecular Biology
Sandra Hauser, Paul Sommerfeld, Johanna Wodtke, Christoph Hauser, Paul Schlitterlau, Jens Pietzsch, Reik Loeser, Markus Pietsch, Robert Wodtke
Summary: Transglutaminase 2 plays a crucial role in tumor cells, and quantifying its activity is important for cancer treatment. This study developed a fluorescence anisotropy-based assay for the quantitative expression profiling of TGase 2, providing a simple, homogeneous, and continuous method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Multidisciplinary
Ji-Eun Lee, Hee Yeon Cho, Jae Hoon Lee, Dong Uk Ahn, Kee-Tae Kim, Hyun-Dong Paik
Summary: This study investigated the inhibitory effects of ovomucoid, a protein found in egg white, on the biofilm formation of Streptococcus mutans, the primary pathogen associated with tooth decay. The results showed that ovomucoid inhibited biofilm formation, reduced bacterial adhesion and aggregation, and lowered the metabolic activity of bacteria in the biofilm. In addition, ovomucoid decreased the synthesis of water-insoluble EPS by S. mutans. Therefore, ovomucoid has the potential to be used as an anti-biofilm agent for the treatment of dental caries.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Elisabeth Anne Adanma Obara, Birgitte Georg, Jens Hannibal
Summary: Pituitary adenylate cyclase-activating polypeptide (PACAP) plays a crucial role in migraine pathophysiology. We have developed a sandwich ELISA assay capable of detecting and accurately quantifying PACAP.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Jianlei Shen, Bo Situ, Xianchao Du, Zhiming Wang, Rong Hu, Baixue Li, Anjun Qin, Ben Zhong Tang
Summary: This study presents a strategy to improve the detection sensitivity and broaden the dynamic range of enzyme-linked immunosorbent assay (ELISA) by replacing traditional chromogenic reagents with an aggregation-induced emission luminogen (AIEgen). The developed AIE-ELISA showed a lower limit of detection and a broader dynamic range compared to single-mode ELISA in the detection of prostate-specific antigen (PSA), allowing for differentiation of minor PSA differences in patient's serum. The AIE-ELISA demonstrated simpler experimental operation, faster enzyme response speed, and better photostability than traditional chromogenic reagents, making it highly promising in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Christopher T. Sempos, Ernst Lindhout, Nicolas Heureux, Michel Hars, Damon A. Parkington, Emily Dennison, Ramon Durazo-Arvizu, Kerry S. Jones, Stephen A. Wise
Summary: The majority of circulating 25-hydroxyvitamin D (25(OH)D) is protein bound. The measurement of free 25(OH)D in human serum is proposed as a better indicator of vitamin D health status. A new enzyme-linked immunosorbent assay (ELISA) provides a method for direct measurement of non-protein bound 25(OH)D. The ELISA was used to measure free 25(OH)D in three existing Standard Reference Materials (SRMs).
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Review
Materials Science, Multidisciplinary
Qian Wu, Bingjian Zhang, Yulan Hu
Summary: The analysis of historical materials plays a crucial role in the preservation of cultural heritage. By examining protein-based materials, it is possible to determine their source, investigate the manufacturing technology, and identify deterioration mechanisms. Currently, mass spectrometry, chromatography, spectroscopy, proteomics, and immunoassay are commonly used to analyze protein materials. This article discusses the evolution of protein component detection in ancient materials, as well as the application of proteomics and ELISA techniques for protein analysis.
Article
Biochemistry & Molecular Biology
Seiichi Sakamoto, Kei Minami, Poomraphie Nuntawong, Gorawit Yusakul, Waraporn Putalun, Hiroyuki Tanaka, Shunsuke Fujii, Satoshi Morimoto
Summary: Bioimprinting using ovalbumin (OVA) as a template was successfully performed to create binding sites for kwakhurin (Kwa). This allowed the development of novel techniques, icELISA and cELBIA, for quantitative analysis of Kwa in P. candollei and related products. Additionally, bioimprinting using other proteins demonstrated the potential of monoclonal antibodies (MAbs) as probes for Kwa analysis, despite a loss in their original binding affinity.
