Article
Biochemistry & Molecular Biology
Jie Yang, Huan Wang, Yuhang Zhou, Lirong Duan, Karl H. Schneider, Zhaozhu Zheng, Fengxuan Han, Xiaoqin Wang, Gang Li
Summary: The present study introduces a silk microfiber reinforced meniscus scaffold (SMRMS) made from silk fibroin (SF) and wool keratin (WK) using electrospinning and freeze-drying technology. The scaffold exhibits a hierarchical fibrous and porous structure with varying pore sizes. It possesses robust mechanical properties, stable biodegradability, and shows no cytotoxicity, making it a promising candidate for meniscal repair engineering.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Edward R. Floyd, Ariel N. Rodriguez, Kari L. Falaas, Gregory B. Carlson, Jorge Chahla, Andrew G. Geeslin, Robert F. LaPrade
Summary: This study discusses the impact of posterior medial meniscus root tears (PMMRTs) on the progression of osteoarthritis (OA) and the consequences of nonanatomic root repairs, finding that the injuries lead to an increase in contact pressure in the medial compartment and a decrease in contact area. Through a case series, it is discovered that nonanatomic repairs can result in meniscal extrusion and symptomatic meniscal extrusion.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Megan K. DeBari, Xiaodan Niu, Jacqueline V. Scott, Mallory D. Griffin, Sean R. Pereira, Keith E. Cook, Bin He, Rosalyn D. Abbott
Summary: The study reveals that therapeutic ultrasound can noninvasively trigger enhanced degradation of silk fibroin scaffolds by removing microbubbles on the scaffold surface. This ultrasound-responsive biomaterial is proven safe for human cells and effectively triggers scaffold degradation through human skin, increasing clinical relevance. The degradation profile can be adjusted noninvasively to improve regenerative outcomes.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Engineering, Biomedical
Zhou Jian, Tian Zhuang, Tian Qinyu, Peng Liqing, Li Kun, Luo Xujiang, Wang Diaodiao, Yang Zhen, Jiang Shuangpeng, Sui Xiang, Huang Jingxiang, Liu Shuyun, Hao Libo, Tang Peifu, Yao Qi, Guo Quany
Summary: By optimizing the preparation of meniscus-derived bioink and utilizing a custom 3D bioprinting system, a biomimetic meniscal scaffold with optimal features in terms of morphology and components was successfully created. Comprehensive evaluations were conducted on cell viability, mechanics, biodegradation, and tissue formation to ensure the scaffold's feasibility and functionality for application in tissue engineering.
BIOACTIVE MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Ying Shen, Xinyu Wang, Binbin Li, Yajin Guo, Kuo Dong
Summary: This study incorporated nanoparticles into a scaffold to improve hemostatic properties and stimulate cell adhesion. The scaffold loaded with nanoparticles exhibited higher blood clotting index and promoted platelets adhesion. In addition, the scaffold loaded with nanoparticles showed stimulation effects on cell proliferation, migration, and adhesion. Therefore, the scaffold loaded with nanoparticles has great potential as a wound dressing.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biotechnology & Applied Microbiology
Jay Trivedi, Daniel Betensky, Salomi Desai, Chathuraka T. Jayasuriya
Summary: Surgical repair of meniscus injury aims to alleviate pain, prevent exacerbation of the injury, restore normal knee function, and inhibit accelerated development of post-traumatic osteoarthritis. Current surgical approaches do not eliminate the risk of accelerated PTOA. Innovative strategies utilizing biologics, allografts, and scaffolds are being explored for potential treatments, but the impact on PTOA development is often overlooked.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Ping-Huei Tsai, Chin-Chean Wong, Wing P. Chan
Summary: This study validated a 2D radial T2* mapping method for revealing subtle alterations in the menisci of patients with knee osteoarthritis (OA). The results showed that the radial T2* mapping method provided better discrimination than the conventional method. It was found that the T2* values in the menisci were significantly higher in the OA group compared to the control group, and there were correlations between the T2* values and the Lequesne index.
EUROPEAN RADIOLOGY
(2022)
Review
Orthopedics
Hongwei Zhan, Zhongcheng Liu, Yaobin Wang, Yi Chen, Fei Teng, Ao Yang, Yuchen Tang, Yayi Xia, Meng Wu, Jin Jiang
Summary: This study aims to systematically review and meta-analyze the factors associated with concomitant medial meniscus extrusion (MME) in patients with osteoarthritis (OA) or medial meniscal tears (MMT). Results showed that radiographic OA, bone marrow lesions, cartilage damage, and higher body mass index (BMI) were significantly associated with MME in OA patients, while medial meniscal root and radial tears were significantly associated with increased risk of MME in patients with MMT.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Engineering, Biomedical
Gerardo E. Narez, Feng Wei, Loic Dejardin, Roger C. Haut, Tammy L. Haut Donahue
Summary: The study showed that P188 can effectively reduce cell death in injured meniscal tissue, regardless of whether it is administered once or multiple times. After 14 days of experimentation, all treatment groups showed similar levels of cell apoptosis and proteoglycan coverage to the unimpacted samples.
JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Haojiang Xie, Qiao Bai, Fankai Kong, Yang Li, Xiaoying Zha, Lingqin Zhang, Yiming Zhao, Shasha Gao, Ping Li, Qifeng Jiang
Summary: The study has successfully developed a transparent bio-functionalized cutaneous scaffold with excellent biocompatibility and wound healing promotion, showing great potential for clinical treatment of cutaneous wounds.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Tao Wang, Yuqi Li, Jian Liu, Yan Fang, Wenjun Guo, Yu Liu, Xiangyu Li, Gang Li, Xiuli Wang, Zhaozhu Zheng, Xiaoqin Wang, David L. Kaplan
Summary: A silk fibroin hydrogel with high elasticity, anti-fatigue properties, and stable structure and mechanical strength in aqueous solution was prepared using a chemical crosslinker. The hydrogel showed biocompatibility and served as a bio-lubricant for the treatment of osteoarthritis.
Article
Chemistry, Multidisciplinary
Hao Miao, Runqing Shen, Wenhai Zhang, Zaifu Lin, Hao Wang, Likun Yang, Xiang-Yang Liu, Naibo Lin
Summary: Silk fibroin scaffolds with a near-infrared light response show significant cytotoxicity to MG63 cancer cells, making them potentially useful for bone tumor treatment. The superior structure and performance of these scaffolds also demonstrate great potential for photothermal therapy applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Sport Sciences
Stine Haugaard Clausen, Soren T. Skou, Mikael Ploug Boesen, Dimitar Ivanon Radev, Engin Yeter Kurt, Camma Damsted, Per Holmich, Martin Lind, Sofus Torring, Christin Isaksen, Claus Varnum, Martin Englund, Jonas Bloch Thorlund
Summary: This study aimed to compare the differences in structural knee joint damage and patient-reported outcomes in young adults with a meniscal tear treated with early surgery or exercise and education with optional delayed surgery. The study found that the worsening of structural damage assessed by MRI was similar between the two treatment groups, and both groups had similar improvements in patient-reported outcomes.
BRITISH JOURNAL OF SPORTS MEDICINE
(2023)
Article
Engineering, Environmental
Guocheng Ding, Xinpan Li, Muyang Sun, Yangyang He, Fengyuan Zhao, Tong Wu, Junyan Wang, Shuang Ren, Weili Shi, Luzheng Xu, Xiaoqing Hu, Wei Huang, Ran Yu, Yingfang Ao
Summary: This study presents a novel tissue engineering meniscus scaffold prepared using 3D printing technology, which shows great potential in promoting meniscus regeneration, protecting articular cartilage, and mitigating the development of osteoarthritis. It addresses the challenges posed by polyurethane in existing meniscus grafts and provides a next-generation clinical meniscus biomaterial repair strategy.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Orthopedics
Amin Mohamadi, Kaveh Momenzadeh, Aidin Masoudi, Kempland C. Walley, Kenny Ierardi, Arun Ramappa, Joseph P. DeAngelis, Ara Nazarian
Summary: This study provides a chronological overview of meniscus biomechanics research over the past forty years, highlighting the evolution and understanding of this field. From cadaveric studies in the 1970s to finite element modeling and kinematic studies in more recent decades, research has progressed and expanded, paving the way for future directions in this area of study.
BMC MUSCULOSKELETAL DISORDERS
(2021)
Article
Orthopedics
Bjoern H. Drews, Oliver Kessler, Wolfgang Franz, Maren Freutel, Lutz Duerselen
Summary: This study investigated the biomechanical function of anatomic ALL reconstruction in the setting of a combined ACL and ALL injury and reconstruction. The results showed that combined ACL and ALL reconstruction significantly improved the rotatory stability of the knee.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Orthopedics
Andreas M. Seitz, Janina Leiprecht, Jonas Schwer, Anita Ignatius, Heiko Reichel, Thomas Kappe
Summary: The purpose of this study was to investigate the potential of a doubled semitendinosus (ST) and a single gracilis tendon (GT) lateral meniscus autograft to restore knee joint kinematics and tibiofemoral contact after total lateral meniscectomy (LMM). The results showed that the ST autograft significantly improved knee joint kinematics and restored tibiofemoral contact mechanics, while the GT autograft had limited effectiveness.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Immunology
Melanie Haffner-Luntzer, Birte Weber, Kazuhito Morioka, Ina Lackner, Verena Fischer, Chelsea Bahney, Anita Ignatius, Miriam Kalbitz, Ralph Marcucio, Theodore Miclau
Summary: Clinical and preclinical data suggest accelerated bone fracture healing in subjects with an additional traumatic brain injury (TBI). Mechanistically, altered metabolism and neuro-endocrine regulations have been shown to influence bone formation after combined fracture and TBI, thereby increasing the bone content in the fracture callus. However, the early inflammatory response towards fracture and TBI has not been investigated in detail so far.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jana Riegger, Helga Joos, Valentin Moehler, Frank Leucht, Katrin Rading, Christian Kubisch, Anita Ignatius, Markus Huber-Lang, Rolf E. Brenner
Summary: The study suggests that the terminal complement complex (TCC) plays a role in the pathogenesis of posttraumatic osteoarthritis (PTOA). However, the absence of TCC may protect against OA progression and have minor effects on the micro-structure of the subchondral bone.
Article
Endocrinology & Metabolism
Mubashir Ahmad, Nadine Stirmlinger, Irfana Jan, Ulrich Stifel, Sooyeon Lee, Marcel Weingandt, Ulrike Kelp, Juergen Bockmann, Anita Ignatius, Tobias M. Boeckers, Jan Tuckermann
Summary: Mutations in the postsynaptic scaffold protein Shank2 are associated with autism spectrum disorders (ASD) and an increased risk of fractures in affected patients. This study investigates the direct role of Shank2 in regulating bone mass. The findings indicate that Shank2 is expressed in bone and plays a crucial role in osteoblast differentiation and bone formation. The interaction between Shank2 and proteins known from the central nervous system is identified as important for osteoblast differentiation.
Article
Biochemistry & Molecular Biology
Franziska Widmayer, Cornelia Neidlinger-Wilke, Fiona Witz, Jan U. Jansen, Anita Ignatius, Melanie Haffner-Luntzer, Graciosa Q. Teixeira
Summary: Postmenopausal women have an increased risk of intervertebral disc degeneration, possibly due to decreased estrogen levels. This study investigated the effects of 17-beta-estradiol (E2) and low-magnitude, high-frequency vibration (LMHFV) on intervertebral discs (IVDs). The results showed that E2 treatment had minor effects on gene expression, cellularity, and matrix metabolism, while LMHFV alone led to cell loss and increased production of IL-6. However, the combination of E2 and LMHFV had a protective effect against cell loss and decreased IL-6 production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Verena Fischer, Jasmin Maria Buelow, Benjamin Thilo Krueger, Deniz Ragipoglu, Anna Vikman, Melanie Haffner-Luntzer, Konstantinos Katsoulis-Dimitriou, Anne Dudeck, Anita Ignatius
Summary: Previous studies have shown that an increase in mast cells in the bone marrow is associated with age-related and post-menopausal osteoporosis. However, this study found that mast cell-derived RANKL does not contribute to the development of post-menopausal osteoporosis, suggesting that other factors may be responsible for this condition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Melanie Haffner-Luntzer, Deniz Ragipoglu, Mubashir Ahmad, Astrid Schoppa, Lena Steppe, Verena Fischer, Julia Luther, Timur Yorgan, Ernesto Bockamp, Michael Amling, Thorsten Schinke, Anita Ignatius
Summary: Despite improvements in fracture care, a percentage of fractures still heal poorly. The study investigated the potential of Wnt1 as a molecule to improve bone fracture healing. The results showed that Wnt1 promotes bone formation during fracture healing through YAP/BMP signaling. This suggests that Wnt1 could be used as a therapeutic agent for orthopedic complications.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Review
Endocrinology & Metabolism
Merle Stein, Florent Elefteriou, Bjorn Busse, Imke A. K. Fiedler, Ronald Young Kwon, Eric Farrell, Mubashir Ahmad, Anita Ignatius, Liam Grover, Liesbet Geris, Jan Tuckermann
Summary: Major achievements in bone research have relied on animal models and in vitro systems. However, the ethical debate surrounding animal experimentation and advancements in in vitro and in silico techniques have challenged their use. This review provides an overview of the strengths and limitations of animal models, as well as in vitro and in silico technologies, in skeletal research. The authors propose that a combination of the right animal model and state-of-the-art technology is essential for advancing bone research.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Engineering, Biomedical
Lena Schroeter, Friederike Kaiser, Anna-Lena Preissler, Philipp Wohlfahrt, Oliver Kueppers, Uwe Gbureck, Anita Ignatius
Summary: To address the challenges of time-consuming and error-prone preparation of hydroxyapatite (HA) cements for bone defect treatment, researchers have developed a prefabricated magnesium phosphate cement paste based on glycerol. This ready-to-use paste can be directly applied during surgery and shows a promising degradation rate and support for bone regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Orthopedics
Matthias Sukopp, Maoz Shemesh, Elena Pruech, Eran Linder-Ganz, Scott Hacker, Vincenzo Condello, Jonas Schwer, Anita Ignatius, Lutz Duerselen, Andreas Martin Seitz
Summary: The purpose of this in-vitro study was to examine the kinematics of an artificial, free-floating medial meniscus replacement device under dynamic loading situations and different knee joint states. A dynamic knee simulator was used to perform dynamic loading exercises on left human cadaveric knee joints to investigate the knee joint and implant kinematics. The results showed that the non-anchored free-floating device remains within the medial knee joint gap under challenging dynamic loading situations without indicating any luxation tendencies.
JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
(2023)
Article
Endocrinology & Metabolism
Lena Steppe, Benjamin Krueger, Miriam Eva Angelica Tschaffon-Mueller, Jan-Moritz Ramge, Astrid Schoppa, Anita Ignatius, Melanie Haffner-Luntzer
Summary: External mechanostimulation induced by LMHFV has different effects on fracture healing in estrogen-competent and estrogen-deficient rodents. ER alpha signaling in osteoblasts is required for these effects, with ligand-dependent and -independent ER alpha signaling playing different roles. The AF-2 domain of the ER alpha receptor is crucial for the negative effects of vibration during bone fracture healing in estrogen-competent mice, suggesting that the osteoanabolic effects of vibration are mediated by ligand-independent ER alpha signaling.
Article
Orthopedics
Luisa de Roy, Kerstin Eichhorn, Martin Faschingbauer, Klaus Schlickenrieder, Anita Ignatius, Andreas Martin Seitz
Summary: The purpose of this in vitro study was to investigate the effect of hyaluronic acid supplementation on knee joint friction during osteoarthritis progression. The results showed that knee joint friction does not increase with degeneration, and hyaluronic acid supplementation does not lead to an initial decrease in friction.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Meeting Abstract
Dermatology
P. Maity, K. Singh, L. Krug, A. Koroma, W. Bloch, S. Kochanek, M. Wlaschek, M. Schorpp-Kistner, P. Angel, A. Ignatius, H. Geiger, K. Scharffetter-Kochanek
EXPERIMENTAL DERMATOLOGY
(2023)