期刊
KIDNEY INTERNATIONAL
卷 84, 期 3, 页码 521-531出版社
ELSEVIER SCIENCE INC
DOI: 10.1038/ki.2013.114
关键词
ischemic acute kidney injury; mesenchymal stem cells; regulatory T cells; spleen
资金
- National Basic Research Program of China [2011CB964904, 2011CB944000]
- National Natural Science Foundation of China [81270819]
- Fund of Chinese PLA 12th Five-Year Plan for Medical Sciences [BWS11J027]
- National High Technology Research [2011AA020115]
- Key Project in the National Science & Technology Pillar Program in the 12th Five-Year Plan period [2011BAI10B07]
The mechanism of mesenchymal stem cell therapy in acute kidney injury remains uncertain. Previous studies indicated that mesenchymal stem cells could attenuate inflammation-related organ injury by induction of regulatory T cells. Whether regulatory T-cell induction is a potential mechanism of mesenchymal stem cell therapy in ischemic acute kidney injury and how these induced regulatory T cells orchestrate local inflammation are unknown. Here we found that mesenchymal stem cells decrease serum creatinine and urea nitrogen levels, improve tubular injury, and downregulate IFN-gamma production of T cells in the ischemic kidney. In addition to the lung, mesenchymal stem cells persisted mostly in the spleen. Mesenchymal stem cells increased the percentage of regulatory T cells in the spleen and the ischemic kidney. Antibody-dependent depletion of regulatory T cells blunted the therapeutic effect of mesenchymal stem cells, while coculture of splenocytes with mesenchymal stem cells caused an increase in the percentage of regulatory T cells. Splenectomy abrogated attenuation of ischemic injury, and downregulated IFN-gamma production and the induction of regulatory T cells by mesenchymal stem cells. Thus, mesenchymal stem cells ameliorate ischemic acute kidney injury by inducing regulatory T cells through interactions with splenocytes. Accumulated regulatory T cells in ischemic kidney might be involved in the downregulation of IFN-gamma production.
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