Resistance Exercise, Skeletal Muscle FOXO3A, and 85-Year-Old Women

This investigation examined Akt-FOXO3A signaling in young women (YW) and old women (OW) before and after 12 weeks of high-intensity resistance training. Muscle biopsies were taken from the vastus lateralis before and immediately after resistance exercise (RE) in the untrained :And trained states. In response to RE in YW and OW. phospho Akt Thr308 increased in untrained and trained states, with no change on Ser473 site. FOXO3A-Ser253 site was dephosphorylated in untrained state among YW and OW, and nuclear phospho-FOXO3A increased mainly in YW in trained state. In the basal state. OW displayed lower cytosolic phospho-FOXO3A before training, higher total nuclear FOXO3A, and a trend for higher nuclear-to-cytosolie FOXO3A ratio versus YW after 12 weeks. Basal level MuRF-1 and myostatin mRNA decreased in YW, while OW increased myostatin mRNA after 12-weeks. These data suggest that FOXO3A signaling and FOXO3A-related target gene expression are altered in OW and may partially explain the attenuated training adaptations previously reported in these octogenarian women.