Article
Fisheries
Junyan Lin, Lixiang Wei, Minshan Yao, Hong Chen, Ying Tang, Fei Shi, Fanbin Zhan, Yanan Li, Jun Li, Li Lin, Zhendong Qin
Summary: To elucidate the mechanism of EPO-mediated regulation of erythropoiesis, the grass carp EPO gene (GcEPO) was cloned and characterized. The tissue distribution data showed that GcEPO was widely expressed in detected tissues, with the highest expression level in the heart and followed in the liver. Ischemia models upregulated both mRNA and protein expression of GcEPO and modulated downstream signaling pathways. This suggests that GcEPO plays a paramount role in erythropoiesis through modulating the mentioned signaling pathways.
Article
Fisheries
Yuting Qin, Zhaosheng Sun, Wei Wang, Jiawen Xu, Bangjie Wang, Zhao Jia, Xia Li, Junya Wang, Qian Gao, Xinhua Chen, Jun Zou
Summary: CD3 is a crucial component of the TCR-CD3 complex in adaptive immunity, with its homologues CiCD3γ/δ and CiCD3ε identified in grass carp and shown to be highly expressed in healthy fish and modulated by infections. A monoclonal antibody against CiCD3γ/δ was successfully produced, which showed good reactivity in Western blotting and flow cytometry analysis. The CiCD3γ/δ(+) cells were found to be localized in the gills and head kidney, accounting for a small percentage of lymphocytes in different organs.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Chuxin Wu, Hang Deng, Dongming Li, Lihua Fan, Dong Yao, Xiaoping Zhi, Huiling Mao, Chengyu Hu
Summary: In this study, grass carp Tollip (CiTollip) showed a faster and stronger response to LPS stimulation compared to poly I:C treatment, reducing the expression of tnf-alpha induced by LPS. Except for the truncated mutant of Delta CUE2 (1-173 aa), wild type CiTollip and other truncated mutants were able to associate with MyD88 and negatively regulate its induced NF-kappa B activation, indicating the crucial role of the C-terminal region, especially the connection between the C2 and CUE domains, in suppressing MyD88-induced NF-kappa B activation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yan He, Hongyan Yu, Honggang Zhao, Hua Zhu, Qingjing Zhang, Anqi Wang, Yubang Shen, Xiaoyan Xu, Jiale Li
Summary: The study evaluated the effects of high stocking density on the transcriptome of grass carp, revealing that high stocking density may accelerate lipid metabolism and carbohydrate decomposition, while leading to abnormalities in immune system regulation. Therefore, emphasizing the importance of maintaining reasonable density in grass carp aquaculture.
Article
Biotechnology & Applied Microbiology
Huiqin Niu, Yifan Pang, Lingli Xie, Qiaozhen Yu, Yubang Shen, Jiale Li, Xiaoyan Xu
Summary: This study investigates the evolutionary background and expression regulation of grass carp miRNAs. Multiple novel miRNAs have emerged during grass carp evolution, and both duplication and de novo production contribute to miRNA cluster formation, with de novo creation being more prevalent. Clustered miRNAs are more conserved and change slower, while unique miRNAs tend to have higher evolution rates and lower expression levels.
Article
Fisheries
Guokun Yang, Xiaomin Liang, Shuangyang Xu, Huimin Cai, Limei Ma, Xulu Chang, Yanmin Zhang, Liping Yang, Xiaolin Meng
Summary: This study focused on the involvement of Igf3 in fish reproduction, particularly in grass carp, by isolating the gene and assessing its functions through various experiments. Igf3 was found to regulate gene expression in multiple tissues, affecting growth-related genes and glucose metabolism. This research is the first to reveal the mechanism by which Igf3 regulates the expression of growth-related genes in teleosts.
Article
Fisheries
Chaobin Qin, Wenli Zhao, Xiao Yan, Guokun Yang, Liping Yang, Ronghua Lu, Daming Pi, Guoxing Nie
Summary: The study suggests that adiponectin plays critical roles in regulating glucose metabolism in fish. It promotes glycolysis, gluconeogenesis, and glycogen synthesis, increases glycogen content in the hepatopancreas, and reduces basal blood glucose level. Adiponectin A and adiponectin B have opposite regulatory effects on glucose uptake.
AQUACULTURE NUTRITION
(2022)
Article
Fisheries
Xue-Mei Xiong, Wei-Jie Huang, Qiang Dong, Dong-Yang Zhang, Shi-Ming Wan, Ze-Xia Gao
Summary: This study evaluated the genetic parameters of intermuscular bone (IB) traits in grass carp (Ctenopharyngodon idella), finding low heritability but a strong genetic correlation between body height and IB number.
Article
Fisheries
Shanshan Zeng, Meifeng Li, Xining Cheng, Shina Lu, Zhiqing Feng, Zeyin Jiang, Zhichao Sun, Xiaowen Xu, Huiling Mao, Chengyu Hu
Summary: In this study, it was found that grass carp DYRK2 induces cell apoptosis by increasing the phosphorylation level of p53.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Hong Chen, Minshan Yao, Ying Tang, Lixiang Wei, Junyan Lin, Fei Shi, Fanbin Zhan, Yanan Li, Jun Li, Li Lin, Zhendong Qin
Summary: By culturing grass carp red blood cells in saline solution and L15 medium, the study investigated the changes in cell morphology during cell death. Flow cytometry analysis showed that prolonged exposure in these mediums increased the accumulation of reactive oxygen species and nitric oxide, promoting cell death. Using death inhibitors, it was found that ferroptosis played a key role in the death of these cells.
Article
Biochemistry & Molecular Biology
Pei Li, Youqing Xu, Yupo Cao, Zhaokun Ding
Summary: The present study evaluates the anti-inflammatory activity of Lactococcus lactis BL52 and isolates two active peptides responsible for the activity. The peptides demonstrate a dose-dependent inhibition of inflammatory cytokines and factors in vitro, as well as preventing their upregulation in vivo. The peptides also block a signaling pathway and relieve pathological inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Fisheries
Wei Wei, Junya Wang, Qingyu Min, Zhao Jia, Kangyong Chen, Hao Feng, Jun Zou
Summary: The study shows that CCL19a2 and CCL19b can promote migration of immune cells and are involved in recruitment of antigen presenting cells in fish. These chemokines exhibit activity and significance both in vitro and in vivo.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Fisheries
Ling-Mei Wen, Feng Lin, Jiang Wei-Dan, Pei Wu, Liu Yang, Kuang Sheng-Yao, Shu-Wei Li, Tang Ling, Lu Zhang, Hai-Feng Mi, Xiao-Qiu Zhou
Summary: This 8-week experiment assessed the effect of dietary thiamin on the meat quality of grass carp. The results showed that optimal dietary thiamin improved grass carp flesh quality by increasing nutritional quality, improving flesh flavor, elevating fillet healthcare components contents, enhancing sensory characteristics, and enhancing flesh antioxidative capacity. The changes in muscle flavor, healthcare components, sensory characteristics, antioxidant capacity, target of rapamycin, and Nrf2 expression were correlated with the dietary thiamin.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Zeinab Hassan, Junya Wang, Yuting Qin, Wei Wang, Qin Liu, Lina Lei, Zhaosheng Sun, Yibin Yang, Kaizheng Wu, Xiaozhen Zhu, Zixuan Wang, Hao Feng, Jun Zou
Summary: IL-20, a pleiotropic cytokine, has been cloned in grass carp and shown to be inducible in head kidney leukocytes and macrophages. The recombinant CiIL-20L was able to induce expression of various cytokine and inflammation genes, suggesting a potential regulatory role in promoting immune responses and inflammation.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Minxuan Yang, Zhijie Lu, Fenglin Li, Fei Shi, Fanbin Zhan, Lijuan Zhao, Yanan Li, Jun Li, Li Lin, Zhendong Qin
Summary: The study revealed that fish red blood cells are not only responsible for oxygen transportation, but also potentially play a role in immune response against bacterial infections. After incubation with E. coli, grass carp RBCs showed changes in gene expression, activating various immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2021)
