期刊
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B
卷 14, 期 12, 页码 1070-1083出版社
ZHEJIANG UNIV
DOI: 10.1631/jzus.B1300094
关键词
Pear; Ultrastructure; Ascorbate peroxidase; Subcellular localization; Synergistic effect
类别
资金
- Earmarked Fund for the Modern Agro-industry Technology Research System, China [nycytx-29]
Plants encounter a variety of stresses in natural environments. One-year-old pot-grown trees of pear (Pyrus pyrifolia Nakai cv. Cuiguan and Wonhwang) were exposed to two heat stress regimes. Under constant short-term heat stress, chloroplasts and mitochondria were visibly damaged. Relative chlorophyll content and maximum photochemical efficiency of photosystem II were significantly decreased, which indicated that the leaf photosynthetic capability declined. Under chronic heat stress, mesophyll cell ultrastructure was not obviously damaged, but leaf photosynthetic capability was still restrained. As chronic heat stress was a simulation of the natural environment in summer, further study of the responses under this stress regime was undertaken. Ascorbate peroxidase (APX) activity was increased in 'Cuiguan', but not in 'Wonhwang'. Inducible expression of PpAPX genes in the cytoplasm, chloroplasts and peroxisomes was consistent with increased APX activity in 'Cuiguan', whereas only weak induction of PpAPX genes was observed in 'Wonhwang'. The isoenzymes cytosolic APX1 (cAPX1) and stromal APX (sAPX) were confirmed to be localized in the cytoplasm and chloroplasts, respectively.
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