Article
Surgery
Jose Romo-Yanez, Rosalba Sevilla-Montoya, Edith Perez-Gonzalez, Josiff Flores-Reyes, Estibalitz Laresgoiti-Servitje, Salvador Espino-Sosa, Mauricio Dominguez-Castro, Guadalupe Razo-Aguilera, Alberto Hidalgo-Bravo, Monica Aguinaga-Rios
Summary: The aim of this study was to determine the prevalence of Y-chromosome microdeletions in AZFa, AZFb, AZFc, and gr/gr regions in infertile Mexican patients. The results showed that 15.8% of infertile patients had Y-chromosome microdeletions, while no deletions were found in the control group. Additionally, the neural network model revealed a mild genotype-phenotype correlation between specific deletions and particular types of infertility.
CIRUGIA Y CIRUJANOS
(2022)
Review
Andrology
Chen-Yao Deng, Zhe Zhang, Wen-Hao Tang, Hui Jiang
Summary: Spermatogenesis is regulated by Y chromosome-specific genes located in the AZF region. AZF microdeletions are structural chromosomal abnormalities that cause male infertility. This review summarizes the mechanism of AZF microdeletion, the function of candidate genes, and the impact of vertical transmission on male fertility.
ASIAN JOURNAL OF ANDROLOGY
(2023)
Article
Genetics & Heredity
Saurav Dutta, Pranab Paladhi, Samudra Pal, Gunja Bose, Papiya Ghosh, Ratna Chattopadhyay, Baidyanath Chakravarty, Sujay Ghosh
Summary: This study conducted screening for Y chromosome microdeletion in infertile male cohort from West Bengal, India and found that microdeletions were more common in azoospermic males. The findings suggest that Y chromosome microdeletion plays a significant role in the genetic etiology of infertility among Bengali men.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2021)
Article
Cell Biology
Haiming Cao, Zi Wan, Fei Wang, Ziyin Liu, Xiaofeng Li, Jianquan Hou
Summary: The study identified 27 shared genes associated with spermatogenesis through reanalyzing gene expression databases and validation datasets. Among these genes, KIF2C and TEKT2 were found to be down-regulated in spermatogenesis and validated in multiple datasets. The study also established a TF-miRNA-target gene network, suggesting that KIF2C may play a role in spermatogenesis through interactions with miRNAs and TFs.
Review
Biochemistry & Molecular Biology
Maria-Anna Kyrgiafini, Zissis Mamuris
Summary: This systematic review investigates the differential expression of circRNAs in male infertility and their potential as candidate biomarkers. It was found that many circRNAs are deregulated in male infertility and their interactions with miRNAs play a crucial role in cellular processes and pathways. CircRNAs could also be used as biomarkers for screening patients before sperm retrieval, but there is a knowledge gap regarding their role in other subtypes of male infertility.
Article
Endocrinology & Metabolism
Jian Song, Yanwei Sha, Xiaojun Liu, Xuhui Zeng, Xiuling Zhao
Summary: Non-obstructive azoospermia (NOA) affects 10% of infertile men worldwide, and genetic studies have discovered several monogenic mutations responsible for idiopathic NOA cases. This study identified three novel TEX11 mutations in NOA patients and found histological abnormalities in the testicular biopsy specimens. These findings expand the understanding of NOA genetic variants and highlight the importance of TEX11 screening for clinical diagnosis of azoospermia patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Madalina Gabriela Barbu, Dana Claudia Thompson, Nicolae Suciu, Silviu Cristian Voinea, Dragos Cretoiu, Dragos Valentin Predescu
Summary: MicroRNAs play a significant role in male infertility, with their concentration in semen being linked to specific reproductive dysfunctions. Aberrant expression of certain microRNAs has been associated with male reproductive disorders. Additionally, idiopathic male infertility is connected to single nucleotide polymorphisms in microRNA binding sites.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chying-Chyuan Chan, Te-Hsin Yen, Hao-Chen Tseng, Brang Mai, Pin-Kuan Ho, Jian-Liang Chou, Gwo-Jang Wu, Yu-Chuan Huang
Summary: Advanced reproductive technologies have been used to identify genetic mutations associated with male infertility. This study identified potential single nucleotide polymorphisms (SNPs) related to male infertility through targeted next-generation sequencing (NGS) and whole exome sequencing (WES). The validation step of Sanger sequencing added confidence to the identified variants. Mutations in SPATA16, CFTR, KIF6, STPG2, and DRC7 were found to be associated with male infertility, particularly azoospermia. These findings suggest that dysfunction of microtubule-related and spermatogenesis-specific genes contributes to idiopathic male infertility and further investigations are needed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Applied
Busra Demir, Murat Gurbuz, Jale Catak, Halime Ugur, Erman Duman, Yavuz Beceren, Mustafa Yaman
Summary: B group vitamins, except folate, are involved in cellular energy production. Vegetables are essential for a healthy diet as they contribute to the intake of B group vitamins. The studied vegetables showed limited levels of vitamins B-1, B-2, and B-3, with low bioaccessibility.
Article
Genetics & Heredity
Seifeldin Sadek, Tamar Matitashvili, Reem Sharaf Alddin, Bijan Morshedi, Hadi Ramadan, Sunita Dodani, Silvina Bocca
Summary: This study aims to assess the differences in IVF outcomes between couples with obstructive azoospermia (OA), non-obstructive azoospermia (NOA), and male factor (MF). The study found that patients with NOA had a higher chance of live birth after frozen embryo transfers (FET), while those with OA had a lower chance. Additionally, all three subgroups had lower live birth rates (LBR) after fresh embryo transfers (ET) compared to FETs.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Obstetrics & Gynecology
Oktay Ozman, Suleyman Tosun, Numan Bayazit, Sami Cengiz, Mustafa Emre Bakircioglu
Summary: The study aimed to evaluate the efficacy of second micro-TESE in men with nonobstructive azoospermia after failed first micro-TESE. Sperm retrieval was successful in 18.4% of men with the second micro-TESE. Patients with successful sperm recovery had smaller testicular volumes, and those with Leydig cell hyperplasia and tubular sclerosis had higher sperm retrieval rates.
FERTILITY AND STERILITY
(2021)
Article
Obstetrics & Gynecology
Bita Moudi, Azam Asemi-Rad, Nadia Sheibak, Zahra Heidari, Hamidreza Mahmoudzadeh-Sagheb
Summary: This study investigated the association between CHD5 gene variants and male infertility risk using the tetra primer-ARMS-PCR method. The results revealed that the rs12067480 T and rs2273032 A alleles were associated with an increased risk of male infertility. Additionally, the interaction of certain genotypes and haplotypes further increased the risk of infertility. These findings contribute to the understanding of the genetic factors underlying male infertility and have implications for its diagnosis and treatment.
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
(2023)
Article
Endocrinology & Metabolism
Jianmin Xu, Qingling Yang, Wenhui Chen, Yuqing Jiang, Zhaoyang Shen, Huan Wang, Yingpu Sun
Summary: This study aimed to investigate the clinical and singleton newborn outcomes of ICSI-ET using diverse sperm sources (ejaculate, epididymis, and testis) in patients with Oligoasthenospermia, obstructive azoospermia (OA), or non-obstructive azoospermia (NOA). The study found that different sperm sources did not appear to influence clinical pregnancy rates, live birth rates, or newborn outcomes, except for a higher rate of low birth weight in singleton pregnancies using epididymal sperm in OA patients.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Andrology
Come Perouse, Jean Philippe Klein, Sylvain Piqueres, Mohamed Ghazi, Isabelle Aknin, Michele Cottier, Aurelien Scalabre, Lionel Mery
Summary: The study suggests that it is unnecessary to wait for three months before starting infertility assessment for azoospermic patients.
Article
Obstetrics & Gynecology
Ryan Lee, Luke Witherspoon, Meghan Robinson, Jeong Hyun Lee, Simon P. Duffy, Ryan Flannigan, Hongshen Ma
Summary: A machine learning algorithm was developed to detect rare human sperm in semen and microsurgical testicular sperm extraction (microTESE) samples using bright-field (BF) microscopy for nonobstructive azoospermia patients. The algorithm achieved high precision and sensitivity in both validation and testing stages.
FERTILITY AND STERILITY
(2022)
Article
Endocrinology & Metabolism
Meng Rao, Huawei Wang, Shuhua Zhao, Jiang Liu, Ya Wen, Zhao Wu, Zexing Yang, Cunmei Su, Zhenfang Su, Kunhua Wang, Li Tang
Article
Endocrinology & Metabolism
Meng Rao, Zexing Yang, Cunmei Su, Zihan Zhao, Ruixue Wan, Jiang Liu, Youlin Yao, Zhenfang Su, Kunhua Wang, Li Tang, Shuhua Zhao
Summary: Paternal subclinical hypothyroidism is associated with worse clinical outcomes after in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI), especially in men aged 35 and above. Further prospective studies and basic research are needed to confirm these results and understand the underlying mechanisms.
Article
Obstetrics & Gynecology
Li Tang, Meng Rao, Wenjuan Yang, Youlin Yao, Qirui Luo, Ling Lu, Longda Wang, Shuhua Zhao
Summary: A high sperm DNA fragmentation index is associated with a decreased fertilization rate and an increased risk of total fertilization failure or low fertilization rate in in vitro fertilization cycles involving men with mild-to-moderate asthenozoospermia.
JOURNAL OF GYNECOLOGY OBSTETRICS AND HUMAN REPRODUCTION
(2021)
Article
Endocrinology & Metabolism
Yingying Wang, Zhengyan Zeng, Shuhua Zhao, Li Tang, Jin Yan, Nianyu Li, Liping Zou, Xiaorong Fan, Chengcheng Xu, Jin Huang, Wei Xia, Changhong Zhu, Meng Rao
Summary: Humanin concentrations in the follicular fluid were significantly lower in PCOS patients with IR than those without IR. Supplementing with humanin analogue (HNG) attenuated increases in fasting plasma glucose and fasting insulin levels in rats with PCOS, and improved phosphorylation of key proteins in granulosa cells. The study suggests potential therapeutic use of HNG in treating PCOS.
Article
Multidisciplinary Sciences
Leqian Yu, Yulei Wei, Jialei Duan, Daniel A. Schmitz, Masahiro Sakurai, Lei Wang, Kunhua Wang, Shuhua Zhao, Gary C. Hon, Jun Wu
Summary: The study developed an effective three-dimensional culture strategy to generate blastocyst-like structures in vitro which resemble human blastocysts. These structures, termed 'human blastoids', are readily accessible, scalable, versatile, and perturbable alternative to blastocysts for studying early human development, understanding early pregnancy loss, and gaining insights into early developmental defects.
Article
Biochemistry & Molecular Biology
Huishan Wang, Chencheng Yang, Yuwei Li, Shuhua Zhao, Pengcheng Ma, Bingyu Mao
Summary: The study revealed that RNF220 fine tunes Shh signaling by regulating Glis polyubiquitination and nuclear translocation, while also affecting Shh signaling through EED in cerebellar development. In the cytoplasm, RNF220-induced Gli accumulation in the cytoskeletal fraction and degradation via autophagy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Correction
Multidisciplinary Sciences
Leqian Yu, Yulei Wei, Jialei Duan, Daniel A. Schmitz, Masahiro Sakurai, Lei Wang, Kunhua Wang, Shuhua Zhao, Gary C. Hon, Jun Wu
Article
Endocrinology & Metabolism
Longda Wang, Mengxiang Chen, Gaofeng Yan, Shuhua Zhao
Summary: Research suggests that using zona pellucida-bound spermatozoa (ZPBS) in intracytoplasmic sperm injection (ICSI) may result in lower global DNA methylation levels and a higher proportion of autism candidate genes associated with differentially methylated regions (DMRs) compared to manually selected spermatozoa (MSS), potentially increasing the risk of autism in offspring.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Meng Rao, Li Tang, Longda Wang, Mengxiang Chen, Gaofeng Yan, Shuhua Zhao
Summary: This retrospective study compared the effects of density gradient centrifugation (DGC) and swim-up (SU) on the outcomes of in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). After matching and adjustment, no significant differences were found between the DGC and SU groups in terms of sperm recovery rate, progressive motility rate, fertilization rate, embryo quality, and blastocyst formation rate. The cumulative live birth rate and live birth rate per transfer were also not significantly different between the groups. However, the DGC group had a significantly higher fertilization rate when using poor-quality sperm compared to the SU group.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Meng Rao, Longda Wang, Gaofeng Yan, Mengxiang Chen, Li Tang, Shuhua Zhao
Summary: This study found that older men (>= 35 years old) with low-normal paternal fT4 concentrations within the reference range are associated with a decreased live birth rate in assisted reproductive technology outcomes. Future studies are needed to confirm these findings and understand the impact of paternal thyroid function on ART success.
Article
Endocrinology & Metabolism
Shuhua Zhao, Li Tang, Jingyun Fu, Zexing Yang, Cunmei Su, Meng Rao
Summary: This study found that paternal subclinical hypothyroidism is associated with sperm DNA fragmentation in men seeking infertility care.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Endocrinology & Metabolism
Meng Rao, Zhengyan Zeng, Qiaoling Zhang, Cunmei Su, Zexing Yang, Shuhua Zhao, Li Tang
Summary: This study investigated the associations between thyroid autoimmunity (TAI) and embryo quality in women undergoing IVF/ICSI. The results showed that TAI was not associated with embryo quality or clinical outcomes.
Article
Obstetrics & Gynecology
Gaofeng Yan, Youlin Yao, Wenjuan Yang, Ling Lu, Longda Wang, Di Zhao, Shuhua Zhao
Summary: The purpose of this study was to assess the impact of different temperatures and incubation times on the clinical outcomes of FET cycles during the thawing procedure, and to select a better thawing method to improve clinical outcomes. Thawing vitrified embryos at 37 degrees C in all steps with shortening wash time can enhance clinical pregnancy rate (CPR) and implantation rate (IR) in FET cycles. Well-designed prospective studies are warranted to further evaluate the efficacy and safety of the all-37 degrees C thawing method.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Article
Medicine, General & Internal
JIA-HUAN LUO, WEN-ZHEN ZHAO, HUI-XIA LU, QING-YI WANG, HUI-YING WANG, L. I. U. LIU, SHU-HUA ZHAO, RUO-PENG ZHANG
Summary: The clinical outcomes of in vitro maturation (IVM) and in vitro fertilization (IVF) procedures for polycystic ovary syndrome (PCOS) patients are inconsistent. Compared to IVF, IVM has lower rates of oocyte cleavage, excellent-quality embryo, implantation, fertilization, moderate and severe ovarian hyperstimulation syndrome (OHSS), and gonadotropin (Gn) dosage. However, there are no significant differences between IVM and IVF in clinical pregnancy rate, abortion rate, cycle cancellation rate, and birth rate.
ACTA MEDICA MEDITERRANEA
(2022)
Meeting Abstract
Obstetrics & Gynecology
Meng Rao, Shuhua Zhao, Li Tang
FERTILITY AND STERILITY
(2020)