期刊
PROTEOMICS
卷 15, 期 21, 页码 3623-3628出版社
WILEY
DOI: 10.1002/pmic.201400567
关键词
Electrophoresis; Glycoprotein detection; Glycoproteomics; Pre-stain; Pro-Q Emerald 300; UGF202
资金
- National Key Scientific Instrument Development Program of China [2011YQ030139]
- National Nature Science Foundation of China [81201245]
- Zhejiang Provincial Natural Science Foundation [LY13C050002, LQ13H020005]
- Public Projects of Zhejiang Province [2015C33122]
- Science and Technology Program of Longwan [2013YS01]
In this study, a new hydrazide derivative (UGF202) was synthesized and introduced as a highly sensitive and selective fluorescent probe to pre-stain glycoproteins in 1D and 2D SDS-PAGE. As low as 0.5-1 ng glycoproteins (transferrin, alpha 1-acid glycoprotein, avidin) could be selectively detected, which is comparable to that of Pro-Q Emerald 300 stain, one of the most sensitive and commonly used glycoprotein staining kit. In addition, the specificity of the newly developed method was confirmed by the study of de-glycosylation, glycoproteins affinity enrichment and LC-MS/MS, respectively. According to the results, it is concluded that UGF202 pre-stain can provide an alternative for the visualization of gel-separated glycoproteins.
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