期刊
PROTEIN EXPRESSION AND PURIFICATION
卷 109, 期 -, 页码 47-54出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pep.2015.02.002
关键词
Codon optimization; Synthetic gene; Luciferase; Photoprotein; Reporter gene
类别
资金
- JSPS KAKENHI Grant [26462828]
- Research Foundation for Opto-Science and Technology
- Aichi-Gakuin University
- Grants-in-Aid for Scientific Research [26462828] Funding Source: KAKEN
A simple design method for codon optimization of genes to express a heterologous protein in mammalian cells is described. Codon optimization was performed by choosing only codons preferentially used in humans and with over 60% GC content, and the method was named the preferred human codon-optimized method. To test our simple rule for codon optimization, the preferred human codon-optimized genes for six proteins containing photoproteins (aequorin and clytin II) and luciferases (Gaussia luciferase, Renilla luciferase, and firefly luciferases from Photinus pyralis and Luciola cruciata) were chemically synthesized and transiently expressed in Chinese hamster ovary-K1 cells. All preferred human codon-optimized genes showed higher luminescence activity than the corresponding wild-type genes. Our simple design method could be used to improve protein expression in mammalian cells efficiently. (c) 2015 Elsevier Inc. All rights reserved.
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