Substitutions at the cofactor phosphate-binding site of a clostridial alcohol dehydrogenase lead to unexpected changes in substrate specificity

Reconstruction of an Acetogenic 2,3-Butanediol Pathway Involving a Novel NADPH-Dependent Primary-Secondary Alcohol Dehydrogenase

(2014) Michael Köpke et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improving the NADH-cofactor specificity of the highly active AdhZ3 and AdhZ2 from Escherichia coli K-12

(2014) André Pick et al.   JOURNAL OF BIOTECHNOLOGY

POVME 2.0: An Enhanced Tool for Determining Pocket Shape and Volume Characteristics

(2014) Jacob D. Durrant et al.   Journal of Chemical Theory and Computation

Cofactory: Sequence-based prediction of cofactor specificity of Rossmann folds

(2014) Henrik Marcus Geertz-Hansen et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

Crystallographic analysis and structure-guided engineering of NADPH-dependentRalstoniasp. Alcohol dehydrogenase toward NADH cosubstrate specificity

(2013) Alexandra Lerchner et al.   BIOTECHNOLOGY AND BIOENGINEERING

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH

(2013) S. Brinkmann-Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

d-2,3-Butanediol Production Due to Heterologous Expression of an Acetoin Reductase in Clostridium acetobutylicum

(2011) Marco A. J. Siemerink et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

2,3-Butanediol Production by Acetogenic Bacteria, an Alternative Route to Chemical Synthesis, Using Industrial Waste Gas

(2011) Michael Köpke et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications

(2011) Bryan P Tracy et al.   CURRENT OPINION IN BIOTECHNOLOGY

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli

(2011) Sabine Bastian et al.   METABOLIC ENGINEERING

Clostridium ljungdahlii represents a microbial production platform based on syngas

(2010) M. Kopke et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Absolute metabolite concentrations and implied enzyme active site occupancy in Escherichia coli

(2009) Bryson D Bennett et al.   Nature Chemical Biology

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Protein structure prediction on the Web: a case study using the Phyre server

(2009) Lawrence A Kelley et al.   Nature Protocols

Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation

(2008) Stephen W. Ragsdale et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Backrub-Like Backbone Simulation Recapitulates Natural Protein Conformational Variability and Improves Mutant Side-Chain Prediction

(2008) Colin A. Smith et al.   JOURNAL OF MOLECULAR BIOLOGY

GLUE-IT and PEDEL-AA: new programmes for analyzing protein diversity in randomized libraries

(2008) A. E. Firth et al.   NUCLEIC ACIDS RESEARCH