Article
Immunology
Cooper K. Hayes, Christopher K. Villota, Fiona B. McEnany, Stacey Ceron, Sita Awasthi, Moriah L. Szpara, Harvey M. Friedman, David A. Leib, Richard Longnecker, Matthew D. Weitzman, Lisa N. Akhtar
Summary: Clinical HSV-2 isolates collected from neonates with encephalitis are more neurovirulent in human neuronal cell culture and murine models as compared to isolates from neonates with skin-limited disease, suggesting that viral factors contribute to neurologic outcome following human neonatal infection.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Review
Biochemistry & Molecular Biology
Ines Ripa, Sabina Andreu, Jose Antonio Lopez-Guerrero, Raquel Bello-Morales
Summary: The article discusses the role of the HSV-1 viral protein ICP34.5 in controlling cellular antiviral responses and debates its controversial functions such as autophagy inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Virology
Nithya Jambunathan, Carolyn M. Clark, Farhana Musarrat, Vladimir N. Chouljenko, Jared Rudd, Konstantin G. Kousoulas
Summary: HSV-1 and HSV-2 are prototypical alphaherpesviruses that infect neurons and establish lifelong latent infections. They cause orofacial and ocular infections, genital and neonatal infections respectively. The viral glycoproteins bind to cellular receptors to enter cells and mediate cell-to-cell fusion for virus spread. The complex of viral glycoproteins and cellular receptors play a critical role in virus entry and spread.
Article
Hematology
Moses M. Kasembeli, Efiyenia Kaparos, Uddalak Bharadwaj, Ahmad Allaw, Alain Khouri, Bianca Acot, David J. Tweardy
Summary: STAT3 mutations in the DNA-binding domain and Src-homology 2 domain have been found to cause immunodeficiency, malignancy, and autoimmunity. The study reveals that these mutations affect the stability of STAT3 monomer and homodimer, as well as its activation processes. Specifically, mutations in the DNA-binding domain result in reduced DNA binding, while mutations in the Src-homology 2 domain lead to increased DNA binding. Furthermore, mutations related to immunodeficiency show decreased conformational stability, affecting various activation events. Interventions targeting these mutations may have therapeutic potential.
Article
Virology
Carmen Elena Gonzalez, Nawel Ben Abdeljelil, Angela Pearson
Summary: UL24 of HSV-1 plays an important role in virus infection, and its C-terminal domain regulates its nuclear and cytoplasmic localization during infection. Mutations can enhance the accumulation of UL24 in the nucleus, and specific inhibitors can block the nuclear export of UL24.
Article
Biochemistry & Molecular Biology
Sangho D. Yun, Elena Scott, Zahra Moghadamchargari, Arthur Laganowsky
Summary: Ras proteins play a crucial role in cancer as frequently mutated oncogenes. This study investigates the biochemical properties of Ras bound to 2′-deoxyguanosine nucleotides (dGNP). The research reveals differences in the hydrolysis rate of 2′-deoxyguanosine triphosphate compared to its hydroxylated form, and highlights the unexpected abundance of dGNP in K-Ras expressed cells. Furthermore, the study explores the molecular assemblies formed between Ras mutants and SOScat and their dependence on the form of guanosine triphosphate bound to Ras.
Article
Biotechnology & Applied Microbiology
M. Bakkali
Summary: The acquisition of new DNA can lead to drug resistance in bacteria, with some Pasteurellaceae and Neisseriaceae species preferring specific sequence tags. These sequences are over-represented in the genomes of corresponding species, with varied genomic densities and sequences types between families. Phylogenetic and nucleotide-by-nucleotide analyses can infer possible ancestral sequences in these families.
Article
Virology
Beibei Zhang, Juntao Ding, Zhenghai Ma
Summary: It has been demonstrated that the HSV-1 IE gene ICP4 inhibits PKA phosphorylation to induce Rap1b-activation-mediated viral infection. Rap1b activation and membrane enrichment occur early in HSV-1 infection and remain active during viral proliferation. Inhibition of Rap1b activation derives from phosphorylated PKA and Rap1b mutants with prenylation, promoting viral infection in a dose-dependent manner.
Article
Biochemical Research Methods
Nicholas J. Ose, Brandon M. Butler, Avishek Kumar, I. Can Kazan, Maxwell Sanderford, Sudhir Kumar, S. Banu Ozkan
Summary: This study demonstrates that even a single amino acid mutation can alter the motion of important regions critical to protein function. Disease-associated mutations tend to impact the motion of these critical regions, even when located far away. Additionally, positions highly coupled to the active site are more likely to result in disease when mutated, offering a new tool for predicting pathogenesis.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Danique Beijer, Hong Joo Kim, Lin Guo, Kevin O'Donovan, Ines Mademan, Tine Deconinck, Kristof Van Schil, Charlotte M. Fare, Lauren E. Drake, Alice F. Ford, Andrzej Kochanski, Dagmara Kabzinska, Nicolas Dubuisson, Peter Van den Bergh, Nicol C. Voermans, Richard J. L. F. Lemmers, Silvere M. van der Maarel, Devon Bonner, Jacinda B. Sampson, Matthew T. Wheeler, Anahit Mehrabyan, Steven Palmer, Peter De Jonghe, James Shorter, J. Paul Taylor, Jonathan Baets
Summary: Mutations in the HNRNPA1 gene are a rare cause of ALS and MSP, potentially leading to different pathomechanisms and associations with clinical phenotypes.
Letter
Ophthalmology
Nan Chen, Dong Chen, Jun Cheng
Summary: This case report describes a patient who experienced herpes simplex virus (HSV)-related inflammation in the cornea and retina over a period of more than two decades. The patient underwent penetrating keratoplasty for HSV stromal keratitis in 2004 and vitrectomy for acute retinal necrosis (ARN) in 2021. The viruses remained dormant after keratoplasty for 17 years until reactivation occurred and triggered ARN. One year later, the patient developed HSV epithelial keratitis, but the cornea regained transparency after medical treatment.
OCULAR IMMUNOLOGY AND INFLAMMATION
(2023)
Article
Virology
Nishani Wijesekera, Nicholas Hazell, Clinton Jones
Summary: Corticosteroids and the glucocorticoid receptor (GR) play important roles in regulating herpes simplex virus 1 (HSV-1) infection and latency reactivation. The ICP0 promoter contains multiple transcription factor binding sites and can be transactivated by GR, KLF15, and the synthetic corticosteroid dexamethasone (DEX).
Article
Mathematics, Interdisciplinary Applications
D. E. Rivas-Cisneros
Summary: This paper examines the use of recurrence plots to identify changes in DNA sequences. The results show that the arrangement of codons in the recurrence plot helps identify insertions and deletions. Additionally, the recurrence plot of short codon sequences exhibits a homogeneous structure. The paper also discusses limitations such as the computational slowdown when entering a large number of codons and matrix issues.
Review
Neurosciences
Aslam Abbasi Akhtar, Joshua J. Breunig
FRONTIERS IN CELLULAR NEUROSCIENCE
(2015)
Article
Cell Biology
Joshua J. Breunig, Rachelle Levy, C. Danielle Antonuk, Jessica Molina, Marina Dutra-Clarke, Hannah Park, Aslam Abbasi Akhtar, Gi Bum Kim, Xin Hu, Serguei I. Bannykh, Roel G. W. Verhaak, Moise Danielpour
Correction
Cell Biology
Joshua J. Breunig, Rachelle Levy, C. Danielle Antonuk, Jessica Molina, Marina Dutra-Clarke, Hannah Park, Aslam Abbasi Akhtar, Gi Bum Kim, Xin Hu, Serguei I. Bannykh, Roel G. W. Verhaak, Moise Danielpour
Article
Cell & Tissue Engineering
Aslam Abbasi Akhtar, Jessica Molina, Marina Dutra-Clarke, Gi Bum Kim, Rachelle Levy, William Schreiber-Stainthorp, Moise Danielpour, Joshua J. Breunig
Article
Biotechnology & Applied Microbiology
Benjamin Bakondi, Wenjian Lv, Bin Lui, Melissa K. Jones, Yuchun Tsai, Kevin J. Kim, Rachelle Levy, Aslam Abbasi Akhtar, Joshua J. Breunig, Clive N. Svendseni, Shaomei Wang
Article
Oncology
Lan B. Hoang-Minh, Loic P. Deleyrolle, Dorit Siebzehnrubl, George Ugartemendia, Hunter Futch, Benjamin Griffith, Joshua J. Breunig, Gabriel De Leon, Duane A. Mitchell, Susan Semple-Rowland, Brent A. Reynolds, Matthew R. Sarkisian
Correction
Cell Biology
Joshua J. Breunig, Rachelle Levy, C. Danielle Antonuk, Jessica Molina, Marina Dutra-Clarke, Hannah Park, Aslam Abbasi Akhtar, Gi Bum Kim, Xin Hu, Serguei I. Bannykh, Roel G. W. Verhaak, Moise Danielpour
Editorial Material
Neurosciences
Aslam Abbasi Akhtar, Hannah Park, Joshua J. Breunig
Article
Multidisciplinary Sciences
Liang Xu, Ye Chen, Marina Dutra-Clarke, Anand Mayakonda, Masaharu Hazawa, Steve E. Savinoff, Ngan Doan, Jonathan W. Said, William H. Yong, Ashley Watkins, Henry Yang, Ling-Wen Ding, Yan-Yi Jiang, Jeffrey W. Tyner, Jianhong Ching, Jean-Paul Kovalik, Vikas Madan, Shing-Leng Chan, Markus Muschen, Joshua J. Breunig, De-Chen Lin, H. Phillip Koeffler
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Neurosciences
Tianxiang Huang, Lena H. Nguyen, Tiffany Lin, Xuan Gong, Longbo Zhang, Gi Bum Kim, Matthew R. Sarkisian, Joshua J. Breunig, Angelique Bordey
NEUROSCIENCE RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Gi Bum Kim, David Rincon Fernandez Pacheco, David Saxon, Amy Yang, Sara Sabet, Marina Dutra-Clarke, Rachelle Levy, Ashley Watkins, Hannah Park, Aslam Abbasi Akhtar, Paul W. Linesch, Naomi Kobritz, Swasty S. Chandra, Katie Grausam, Alberto Ayala-Sarmiento, Jessica Molina, Kristyna Sedivakova, Kendy Hoang, Jeremiah Tsyporin, Daniel S. Gareau, Mariella G. Filbin, Serguei Bannykh, Chintda Santiskulvong, Yizhou Wang, Jie Tang, Mario L. Suva, Bin Chen, Moise Danielpour, Joshua J. Breunig
Article
Biochemistry & Molecular Biology
Xianzhi Lin, Marcos A. S. Fonseca, Joshua J. Breunig, Rosario Corona, Kate Lawrenson
Summary: RNA molecules, including mRNAs encoding proteins and noncoding transcripts, function with the help of RNA binding proteins (RBPs). RNA proximity labelling (RPL), a method utilizing proximity-based biotinylation, has been developed to rapidly identify target RNA binding proteins in native cellular contexts. This technique has the potential to uncover novel RNA-protein interactions important for health and disease.
Review
Developmental Biology
Sara Sabet, Joshua J. Breunig
Summary: The mammalian forebrain, one of the most complex structures in known existence, originates from the dynamic behavior of progenitors during embryonic neural development. Lineage tracing methods have been used to study developmental dynamics, but technical limitations have restricted their application in spatial and temporal contexts. Genetic barcoding and high-throughput sequencing methods have provided new insights into lineage relationships. Incorporating computational biology and single-cell sequencing into lineage tracing can offer a higher-resolution view of neural lineages during development and disease processes. Understanding lineage relationships is crucial for the treatment of high-grade brain tumors like glioblastoma multiforme, which require effective approaches to address disease heterogeneity and recurrence. This review discusses the past, present, and future of lineage tracing in the context of development and disease.
DEVELOPMENTAL NEUROSCIENCE
(2023)
Article
Biochemical Research Methods
Alberto E. Ayala-Sarmiento, Naomi Kobritz, Joshua J. Breunig
Article
Biochemical Research Methods
David Rincon Fernandez Pacheco, Sara Sabet, Joshua J. Breunig