Article
Multidisciplinary Sciences
Jingyang Guan, Guan Wang, Jinlin Wang, Zhengyuan Zhang, Yao Fu, Lin Cheng, Gaofan Meng, Yulin Lyu, Jialiang Zhu, Yanqin Li, Yanglu Wang, Shijia Liuyang, Bei Liu, Zirun Yang, Huanjing He, Xinxing Zhong, Qijing Chen, Xu Zhang, Shicheng Sun, Weifeng Lai, Yan Shi, Lulu Liu, Lipeng Wang, Cheng Li, Shichun Lu, Hongkui Deng
Summary: This study demonstrates the chemical reprogramming of human somatic cells to human chemically induced pluripotent stem cells, which exhibit key features of embryonic stem cells. The induction of an intermediate plastic state and inhibition of the JNK pathway are crucial for successful chemical reprogramming.
Article
Oncology
Yilin Bao, Huajun Feng, Feipeng Zhao, Lijun Zhang, Shengen Xu, Conghong Zhang, Chong Zhao, Gang Qin
Summary: In this study, the role of FANCD2 in nasopharyngeal carcinoma (NPC) was investigated. Silencing of FANCD2 affected the sensitivity of CNE-2 cells to ionizing radiation by regulating cell proliferation, apoptosis, and cell cycle distribution, potentially through changes in the expression of NUPR1, FLI1, and FGF21 proteins. These findings provide a promising target for NPC radiotherapy.
Article
Chemistry, Multidisciplinary
Deogil Kim, Byung-Hyun Cha, Jinsung Ahn, Yoshie Arai, Bogyu Choi, Soo-Hong Lee
Summary: Understanding the biophysical relationships between stem cells and applied biomaterials is crucial in controlling stem cell functions. This study highlights the significant role of 3D microenvironment, particularly methacrylated hyaluronic acid (HA) hydrogel, in improving cellular reprogramming into iPSCs. The incorporation of cues from the 3D microenvironment accelerates the reprogramming process, offering potential advantages for translational applications.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Cell & Tissue Engineering
Shijia Liuyang, Guan Wang, Yanglu Wang, Huanjing He, Yulin Lyu, Lin Cheng, Zhihan Yang, Jingyang Guan, Yao Fu, Jialiang Zhu, Xinxing Zhong, Shicheng Sun, Cheng Li, Jinlin Wang, Hongkui Deng
Summary: We have developed a robust and chemically defined reprogramming protocol that significantly shortens the induction time for generating human pluripotent stem cells from somatic cells. This protocol promotes cell proliferation and metabolic activities, leading to a more direct reprogramming process.
Article
Multidisciplinary Sciences
Xianchun Lan, Song Ding, Tianzhe Zhang, Ying Yi, Conghui Li, Wenwen Jin, Jian Chen, Kaiwei Liang, Hengbin Wang, Wei Jiang
Summary: This study reveals that PCGF6 plays a crucial role in determining the lineage specification of human pluripotent stem cells by promoting neuroectoderm differentiation and repressing mesendoderm differentiation. The activation of the SOX2 gene and the repression of the WNT/beta-catenin signaling pathway are key mechanisms involved in this process.
NATURE COMMUNICATIONS
(2022)
Review
Endocrinology & Metabolism
Ila Tewari Jasra, Nerea Cuesta-Gomez, Kevin Verhoeff, Braulio A. Marfil-Garza, Nidheesh Dadheech, A. M. James Shapiro
Summary: This review summarizes the roles and mechanisms of mitochondria in somatic cell reprogramming to iPSCs and the metabolic shift associated with directed differentiation into pancreatic beta-like cells.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Cell & Tissue Engineering
Joonas Sokka, Masahito Yoshihara, Jouni Kvist, Laura Laiho, Andrew Warren, Christian Stadelmann, Eeva-Mari Jouhilahti, Helena Kilpinen, Diego Balboa, Shintaro Katayama, Aija Kyttala, Juha Kere, Timo Otonkoski, Jere Weltner, Ras Trokovic
Summary: Conventional reprogramming methods may lead to heterogeneous reprogramming, while CRISPRa activation can reduce this heterogeneity and achieve high-quality pluripotent reprogramming of human cells.
Article
Biochemistry & Molecular Biology
Sarah Krantz, Young-Mee Kim, Shubhi Srivastava, Joseph W. Leasure, Peter T. Toth, Glenn Marsboom, Jalees Rehman
Summary: Research has shown that there is mitophagy and mitochondrial biogenesis during the differentiation of pluripotent stem cells, impacting metabolic rewiring and increased oxidative metabolism of the cells. During endothelial cell differentiation, mitophagy initiates an increase in mitochondrial biogenesis and oxidative metabolism through transcriptional changes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Xingyu Zhao, Ruowen Zhang, Zitong Song, Kun Yang, Han He, Lianhai Jin, Wei Zhang
Summary: This study found that curcumin, an active ingredient in turmeric, can inhibit the survival and induce apoptosis of human papillomavirus (HPV)-related cervical cancer cells. It also demonstrated that curcumin can inhibit the invasion and migration ability of cervical cancer cells and regulate the expression of E6.
PHYTOTHERAPY RESEARCH
(2023)
Article
Cell Biology
Roberta Bottega, Silvia Ravera, Luisa M. R. Napolitano, Viviana Chiappetta, Nicoletta Zini, Barbara Crescenzi, Silvia Arniani, Michela Faleschini, Giuseppe Cortone, Flavio Faletra, Barbara Medagli, Fabio Sirchia, Martina Moretti, Job de Lange, Enrico Cappelli, Cristina Mecucci, Silvia Onesti, Francesca M. Pisani, Anna Savoia
Summary: Warsaw breakage syndrome (WABS), caused by biallelic mutations of DDX11, results in impaired DNA helicase function and increased chromosomal fragmentation. Phenotypic features partially overlap with Fanconi anemia cells, and further examination reveals altered mitochondrial functionality in WABS cells. This sheds light on impaired mitochondrial phenotype and the pathogenesis of these diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Zixian Huang, Yongju Chen, Rui Chen, Bin Zhou, Yongqiang Wang, Lei Hong, Yuepeng Wang, Jianguang Wang, Xiaoding Xu, Zhiquan Huang, Weiliang Chen
Summary: SERPINB3 is identified as an important target for regulating cisplatin sensitivity in HNSCC induced by HPV infection. Decreased SERPINB3 expression enhances the cytotoxicity of cisplatin by interfering with DNA damage repair and promoting HNSCC cell apoptosis. Using pH-responsive nanoparticles to deliver SERPINB3 small interfering RNA in combination with cisplatin successfully reverses cisplatin chemotherapeutic resistance.
Article
Biochemistry & Molecular Biology
Eun Kyoung Do, Hye Ji Moon, Kyung Taek Kang, Jung Won Yoon, Ye Seul Kim, Jeong Kon Seo, Jae Ho Kim
Summary: The study revealed that Kap1 regulates the pluripotency of ESCs and somatic cell reprogramming by inhibiting Itch-mediated ubiquitination of Oct4, increasing Oct4 stability. This finding may offer new insights for the treatment of stem cell and cell reprogramming-related diseases.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Cell Biology
Sureshkumar Perumal Srinivasan, Harshal Nemade, Anna Cherianidou, Luying Peng, Sara Cruz-Molina, Alvaro Rada-Iglesias, Agapios Sachinidis
Summary: This study reveals that Strip2 interacts with the nucleosome remodeling deacetylase/Tripartite motif-containing 28/Histone deacetylases/Histone-lysine N-methyltransferase SETDB1 (NuRD/TRIM28/HDACs/SETDB1) histone methyltransferase complex and acts as an epigenetic regulator of pluripotency and differentiation in embryonic stem cells by modulating DNA KRAB-ZFPs and the complex.
CELL DEATH DISCOVERY
(2022)
Article
Cell & Tissue Engineering
Kazuto Kimura, Masaya Tsukamoto, Miyuu Tanaka, Mitsuru Kuwamura, Manami Ohtaka, Ken Nishimura, Mahito Nakanishi, Kikuya Sugiura, Shingo Hatoya
Summary: This study developed an efficient strategy to generate ciPSCs from canine PBMCs by adding small-molecule compounds in the medium. The generated ciPSCs demonstrated pluripotency characteristics, maintained a normal karyotype, and could differentiate into cells from three germ layers. This method provides a potential tool for disease modeling and regenerative medicine in veterinary field.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell Biology
Hong-Mei Guan, Wen-Qi Li, Jie Liu, Jue-Yu Zhou
Summary: This study identified that HIF1A-AS2 is significantly increased in cervical cancer and plays a role in promoting cell proliferation, migration, invasion, and suppressing apoptosis through regulation of the HPV16 E6-mediated P53/caspase 9/caspase 3 pathway. This suggests that HIF1A-AS2 may serve as a prognostic factor and contribute to the development and treatment of cervical cancer.
CELLULAR SIGNALLING
(2022)
Article
Multidisciplinary Sciences
Ashley Ulm, Christopher N. Mayhew, Jason Debley, Gurjit K. Khurana Hershey, Hong Ji
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2016)
Article
Cell & Tissue Engineering
Timothy M. Chlon, Sonya Ruiz-Torres, Logan Maag, Christopher N. Mayhew, Kathryn A. Wikenheiser-Brokamp, Stella M. Davies, Parinda Mehta, Kasiani C. Myers, James M. Wells, Susanne I. Wells
Article
Multidisciplinary Sciences
Kenneth Daily, Shannan J. Ho Sui, Lynn M. Schriml, Phillip J. Dexheimer, Nathan Salomonis, Robin Schroll, Stacy Bush, Mehdi Keddache, Christopher Mayhew, Samad Lotia, Thanneer M. Perumal, Kristen Dang, Lorena Pantano, Alexander R. Pico, Elke Grassman, Diana Nordling, Winston Hide, Antonis K. Hatzopoulos, Punam Malik, Jose A. Cancelas, Carolyn Lutzko, Bruce J. Aronow, Larsson Omberg
Article
Cell Biology
Rie Ouchi, Shodai Togo, Masaki Kimura, Tadahiro Shinozawa, Masaru Koido, Hiroyuki Koike, Wendy Thompson, Rebekah A. Karns, Christopher N. Mayhew, Patrick S. McGrath, Heather A. McCauley, Ran-Ran Zhang, Kyle Lewis, Shoyo Hakozaki, Autumn Ferguson, Norikazu Saiki, Yosuke Yoneyama, Ichiro Takeuchi, Yo Mabuchi, Chihiro Akazawa, Hiroshi Y. Yoshikawa, James M. Wells, Takanori Takebe
Article
Multidisciplinary Sciences
Danit Oz-Levi, Tsviya Olender, Ifat Bar-Joseph, Yiwen Zhu, Dina Marek-Yagel, Iros Barozzi, Marco Osterwalder, Anna Alkelai, Elizabeth K. Ruzzo, Yujun Han, Erica S. M. Vos, Haike Reznik-Wolf, Corina Hartman, Raanan Shamir, Batia Weiss, Rivka Shapiro, Ben Pode-Shakked, Pavlo Tatarskyy, Roni Milgrom, Michael Schvimer, Iris Barshack, Denise M. Imai, Devin Coleman-Derr, Diane E. Dickel, Alex S. Nord, Veena Afzal, Kelly Lammerts van Bueren, Ralston M. Barnes, Brian L. Black, Christopher N. Mayhew, Matthew F. Kuhar, Amy Pitstick, Mehmet Tekman, Horia C. Stanescu, James M. Wells, Robert Kleta, Wouter de laat, David B. Goldstein, Elon Pras, Axel Visel, Doron Lancet, Yair Anikster, Len A. Pennacchio
Article
Biochemistry & Molecular Biology
Yanyan Peng, Benjamin Liou, Venette Inskeep, Rachel Blackwood, Christopher N. Mayhew, Gregory A. Grabowski, Ying Sun
HUMAN MOLECULAR GENETICS
(2019)
Article
Cell & Tissue Engineering
Sonya Ruiz-Torres, Marion G. Brusadelli, David P. Witte, Kathryn A. Wikenheiser-Brokamp, Sharon Sauter, Adam S. Nelson, Mathieu Sertorio, Timothy M. Chlon, Adam Lane, Parinda A. Mehta, Kasiani C. Myers, Mary C. Bedard, Bidisha Pal, Dorothy M. Supp, Paul F. Lambert, Kakajan Komurov, Melinda Butsch Kovacic, Stella M. Davies, Susanne Wells
Summary: The study found that genetic mutations in Fanconi anemia (FA) patients can affect cell structure and function, accelerating the process of cancer development. This provides a new direction for exploring SCC prevention.
Article
Cell & Tissue Engineering
Timothy M. Chlon, Emily Stepanchick, Courtney E. Hershberger, Noah J. Daniels, Kathleen M. Hueneman, Ashley Kuenzi Davis, Kwangmin Choi, Yi Zheng, Carmelo Gurnari, Torsten Haferlach, Richard A. Padgett, Jaroslaw P. Maciejewski, Daniel T. Starczynowski
Summary: DDX41 mutations are common in adult MDS, affecting hematopoietic progenitor cell development and cell cycle, leading to bone marrow failure. While monoallelic DDX41 mutations generally do not impact hematopoiesis, some aged mice may develop MDS features. Biallelic DDX41 mutations have been observed in BM from MDS patients, and may play a role in disease progression in the context of monoallelic DDX41 mutations.
Article
Oncology
Madeline Niederkorn, Chiharu Ishikawa, Kathleen M. Hueneman, James Bartram, Emily Stepanchick, Joshua R. Bennett, Ashley E. Culver-Cochran, Lyndsey C. Bolanos, Emma Uible, Kwangmin Choi, Mark Wunderlich, John P. Perentesis, Timothy M. Chlon, Marie-Dominique Filippi, Daniel T. Starczynowski
Summary: USP15 is overexpressed in AML, correlating with KEAP1 protein and NRF2 suppression. Inhibition of USP15 leads to activation of NRF2 and impaired AML cell function. Targeting USP15 catalytic function may selectively impair leukemic progenitor cells while sparing normal hematopoiesis.
Review
Oncology
Talha Badar, Timothy Chlon
Summary: DDX41 mutation is one of the most prevalent predisposition genes in adult myelodysplastic syndrome/acute myeloid leukemia. Most patients with DDX41 mutation do not have a family history of MDS/AML. DDX41 mutation leads to defects in cell growth and viability, likely related to the loss of tumor suppressor function. 70% of DDX41 mutations are associated with MDS/AML, with 25% of mutations being inherited mutations from germline cells. Recent studies suggest that a subgroup of patients with DDX41 mutation in myeloid neoplasms have a better clinical course and long-term survival.
CURRENT HEMATOLOGIC MALIGNANCY REPORTS
(2022)
Article
Gastroenterology & Hepatology
Chao Zheng Li, Hiromi Ogawa, Soon Seng Ng, Xindi Chen, Eriko Kishimoto, Kokoro Sakabe, Aiko Fukami, Yueh-Chiang Hu, Christopher N. Mayhew, Jennifer Hellmann, Alexander Miethke, Nahrin L. Tasnova, Samuel J. Blackford, Zu Ming Tang, Adam M. Syanda, Liang Ma, Fang Xiao, Melissa Sambrotta, Oliver Tavabie, Filipa Soares, Oliver Baker, Davide Danovi, Hisamitsu Hayashi, Richard J. Thompson, S. Tamir Rashid, Akihiro Asai
Summary: The study established a disease model using patient-specific induced pluripotent stem cells (iPSC) and CRISPR genome-editing, which can recapitulate the phenotypes of patients with TJP2 deficiency.
Article
Biology
Ruixue Liu, Richard Moriggl, Dongsheng Zhang, Haifeng Li, Rebekah Karns, Hai-Bin Ruan, Haitao Niu, Christopher Mayhew, Carey Watson, Hansraj Bangar, Sang-wook Cha, David Haslam, Tongli Zhang, Shila Gilbert, Na Li, Michael Helmrath, James Wells, Lee Denson, Xiaonan Han
LIFE SCIENCE ALLIANCE
(2019)
Article
Developmental Biology
Akihiro Asai, Eitaro Aihara, Carey Watson, Reena Mourya, Tatsuki Mizuochi, Pranavkumar Shivakumar, Kieran Phelan, Christopher Mayhew, Michael Helmrath, Takanori Takebe, James Wells, Jorge A. Bezerra
Article
Hematology
Laura Barreyro, Timothy M. Chlon, Daniel T. Starczynowski
Article
Multidisciplinary Sciences
Masaki Kimura, Momoko Azuma, Ran-Ran Zhang, Wendy Thompson, Christopher N. Mayhew, Takanori Takebe