Article
Public, Environmental & Occupational Health
Rajesh Kumar, Shubbir Ahmed, Hilal Ahmad Parray, Supratik Das
Summary: Chikungunya, caused by CHIKV, is a viral infection with long-term sequelae of arthralgia and myalgia, and can be fatal in some patients. Despite decades of research on alphaviruses and arthritis, the mechanisms underlying viral infection and arthritis remain poorly understood.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2021)
Article
Infectious Diseases
Carolina dos Santos Lazari, Mariana Severo Ramundo, Felipe ten-Caten, Clarisse S. Bressan, Ana Maria Bispo de Filippis, Erika Regina Manuli, Isabella de Moraes, Geovana Maria Pereira, Marina Farrel Cortes, Darlan da Silva Candido, Alexandra L. Gerber, Ana Paula Guimaraes, Nuno Rodrigues Faria, Helder I. Nakaya, Ana Tereza R. Vasconcelos, Patricia Brasil, Glaucia Paranhos-Baccala, Ester Cerdeira Sabino
Summary: This study provides important insights into the prognostic evaluation of CHIKF, identifying sub-acute disease as a relevant predictor of evolution to chronic arthritis. Joint pain, edema, and multiple joint involvement were found to be risk factors for chronification.
PLOS NEGLECTED TROPICAL DISEASES
(2023)
Article
Multidisciplinary Sciences
Shorouk El Sayed, Izabel Patik, Naresh S. Redhu, Jonathan N. Glickman, Konstantinos Karagiannis, El Sayed Y. El Naenaeey, Gamal A. Elmowalid, Ashraf M. Abd El Wahab, Scott B. Snapper, Bruce H. Horwitz
Summary: Macrophages play a vital role in regulating intestinal inflammation. This study found that in mice lacking IL10 receptor, the absence of CCR2 can reduce the severity of colitis by affecting the accumulation of immature macrophages. However, it does not have an impact on the inflammatory gene expression in mature macrophages.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Swati Gupta, K. P. Mishra, Rupali Gupta, S. B. Singh
Summary: This review highlights the therapeutic role of andrographolide in alleviating symptoms associated with chikungunya virus (CHIKV) infection, emphasizing its regulatory effects on immunological molecules, virus replication, inflammation, oxidative stress, apoptosis, and arthritis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Wern Hann Ng, Xiang Liu, Zheng L. Ling, Camilla N. O. Santos, Lucas S. Magalhaes, Andrew J. Kueh, Marco J. Herold, Adam Taylor, Joseph R. Freitas, Sandra Koit, Sainan Wang, Andrew R. Lloyd, Mauro M. Teixeira, Andres Merits, Roque P. Almeida, Nicholas J. C. King, Suresh Mahalingam
Summary: This study reveals the importance of FHL1 as a crucial host factor for chikungunya virus (CHIKV) and o'nyong-nyong virus (ONNV) infections. Elevated levels of FHL1 are correlated with acute and chronic CHIKV disease in humans. Using FHL1-/- mice, the authors demonstrate that FHL1 impacts arthritis and myositis after CHIKV and ONNV infections, but has no effect on Ross River virus (RRV) or mayaro virus (MAYV) infections. Furthermore, a CHIKV mutant unable to bind FHL1 is avirulent and provides protection against CHIKV and ONNV challenges.
NATURE COMMUNICATIONS
(2023)
Article
Public, Environmental & Occupational Health
J. Kennedy Amaral, Clifton O. Bingham, Peter C. Taylor, Luis M. Vila, Michael E. Weinblatt, Robert T. Schoen
Summary: Chikungunya virus (CHIKV) infection leads to chikungunya fever (CHIKF) characterized by high fevers, rash, joint pain and other symptoms. Some patients develop chronic chikungunya arthritis (CCA) with long-term joint pain and swelling. This article suggests that mesenchymal stem cells (MSCs) may play a role in the pathogenesis of CCA through epigenetic changes. CHIKV infection can cause these changes in MSCs and their progenies.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Hematology
Zuojia Chen, Jialie Luo, Jian Li, Girak Kim, Andy Stewart, Yuefeng Huang, Chuan Wu
Summary: Intestinal inflammation enhances platelet activation and blood coagulation, with intestinal epithelial damage leading to elevated levels of IL-33 and promotion of platelet activation through 5-HT release. Intestinal IL-33 and peripheral IL-33 have a protective role in acute inflammation.
Article
Immunology
Thaiane Pinto Moreira, Carla Daiane Ferreira de Sousa, Victor Rodrigues de Melo Costa, Celso Martins Queiroz-Junior, Franciele Martins Santos, Caio Santos Bonilha, Lisia Maria Esper, Mauricio Lacerda Nogueira, Thiago Mattar Cunha, Mauro Martins Teixeira, Vivian Vasconcelos Costa, Daniele da Gloria de Souza
Summary: This study describes a novel mouse model of CHIKV infection and evaluates the role of tumour necrosis factor in the disease's pathogenesis. It shows that TNF plays a dual role in viral clearance and driving tissue damage and hypernociception. Therefore, TNF inhibitors may have therapeutic benefits, but viral levels must be monitored and used in combination with antiviral drugs when treating CHIKV-infected patients.
Article
Multidisciplinary Sciences
Yosuke Nagasawa, Masami Takei, Mitsuhiro Iwata, Yasuko Nagatsuka, Hiroshi Tsuzuki, Kenichi Imai, Ken-Ichi Imadome, Shigeyoshi Fujiwara, Noboru Kitamura
Summary: EBV infection in hu-NOG mice may induce erosive arthritis through human osteoclast activation, demonstrating the potential for studying the association of EBV infections with rheumatoid arthritis and human bone metabolism in this mouse model.
Article
Multidisciplinary Sciences
Qiaoqi Sui, Xi Zhang, Chao Chen, Jinghua Tang, Jiehai Yu, Weihao Li, Kai Han, Wu Jiang, Leen Liao, Lingheng Kong, Yuan Li, Zhenlin Hou, Chi Zhou, Chenzhi Zhang, Linjie Zhang, Binyi Xiao, Weijian Mei, Yanbo Xu, Jiayi Qin, Jian Zheng, Zhizhong Pan, Pei-Rong Ding
Summary: Inflammatory conditions affect colorectal cancer patients and hinder their ongoing treatment. This study shows that inflammation interferes with the anti-tumor immune response and inhibits the effectiveness of immune checkpoint blockade therapy. The research also indicates that inflammatory conditions and high neutrophil-to-lymphocyte ratio are clinical features associated with poor response to immune checkpoint inhibitors in MSI-H CRCs.
NATURE COMMUNICATIONS
(2022)
Article
Infectious Diseases
Churnalisa Doran, Jelte Elsinga, Ante Fokkema, Kevin Berenschot, Izzy Gerstenbluth, Ashley Duits, Norediz Lourents, Yaskara Halabi, Johannes Burgerhof, Ajay Bailey, Adriana Tami
Summary: This study found that patients with long-term chikungunya disease are still affected 2.5 years after disease onset. Patients reported mainly recurrent rheumatic and non-rheumatic/psychological symptoms, including fatigue, insomnia, sombreness, and loss of vitality, with significantly higher symptom recurrence and pain intensity reported by highly affected patients. In addition, QoL assessment indicates that the disease burden impaired the physiological well-being of these patients. The CLTCS-score can be used to easily identify highly affected patients.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Multidisciplinary Sciences
Penny A. Rudd, Elisa X. Y. Lim, Catherine J. M. Stapledon, Ravi Krishnan, Lara J. Herrero
Summary: The study provides a detailed analysis of a novel treatment for CHIKV arthritis, showing that PPS can reduce inflammation and protect joint integrity. Mice treated with PPS demonstrated significant functional improvement, decreased joint inflammation, and protected joint cartilage.
Article
Medicine, Research & Experimental
Marina Zieger, Florie Borel, Cynthia Greer, Gwladys Gernoux, Meghan Blackwood, Terence R. Flotte, Christian Mueller
Summary: This study demonstrates that gene therapy can be used to treat alpha(1)-antitrypsin deficiency, improving lung disease progression and restoring lung tissue elasticity and alveolar wall integrity.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2022)
Article
Biochemistry & Molecular Biology
B. M. C. Randika Wimalasiri-Yapa, Roberto A. Barrero, Liesel Stassen, Louise M. Hafner, Elizabeth A. McGraw, Alyssa T. Pyke, Cassie C. Jansen, Andreas Suhrbier, Laith Yakob, Wenbiao Hu, Gregor J. Devine, Francesca D. Frentiu
Summary: Temperature plays a significant role in modulating the mosquito gene expression response to arbovirus infection. Higher temperatures correlated with higher virus levels and altered gene expression in mosquitoes infected with chikungunya virus, particularly at 28 degrees Celsius. The upregulation of immune pathways was only observed at 28 degrees Celsius, indicating temperature-dependent changes in immune responses.
Article
Microbiology
Jessamine E. Hazlewood, Troy Dumenil, Thuy T. Le, Andrii Slonchak, Stephen H. Kazakoff, Ann-Marie Patch, Lesley-Ann Gray, Paul M. Howley, Liang Liu, John D. Hayball, Kexin Yan, Daniel J. Rawle, Natalie A. Prow, Andreas Suhrbier
Summary: The RNA-Seq analysis of poxvirus vaccine vectors at intramuscular injection sites revealed detailed insights into host innate immune responses, as well as expression of vector and recombinant immunogen genes. Multiple adjuvant and cytokine signatures were identified, along with dendritic cell stimulation signatures. By day seven, vaccine transcripts were absent and inflammation had abated, suggesting potential for refining poxvirus-based vector design.
Article
Infectious Diseases
Felipe Ten-Caten, Patricia Gonzalez-Dias, Icaro Castro, Rodrigo L. T. Ogava, Jeevan Giddaluru, Juan Carlo S. Silva, Felipe Martins, Andre N. A. Goncalves, Andre G. Costa-Martins, Jose D. Araujo, Ana Carolina Viegas, Fernando Q. Cunha, Sandra Farsky, Fernando A. Bozza, Anna S. Levin, Pia S. Pannaraj, Thushan de Silva, Paola Minoprio, Fabiano Pinheiro da Silva, Bruno B. Andrade, Helder Nakaya
Summary: The study analyzed laboratory parameters of a large cohort of COVID-19 patients and identified the impact of sex and age on these parameters. Both male and female patients showed similar alterations in laboratory parameters, with older men displaying more significant increases in CRP and ferritin.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Maxence Fretaud, Delphyne Descamps, Daphne Laubreton, Marie-Anne Rameix-Welti, Jean-Francois Eleouet, Thibaut Larcher, Marie Galloux, Christelle Langevin
Summary: The combination of tissue clearing, 3D microscopy, and image processing has led to the development of a novel visualization tool for RSV infection in undissected mouse lungs, allowing for the identification of infected cell subtypes and cytoplasmic viral factories. This new method provides unprecedented insights into RSV pathophysiology and enhances 2D histology analyses.
Article
Immunology
Leonardo Paiva Farias, Juliana Vitoriano-Souza, Lucas Esteves Cardozo, Leonardo Dos Reis Gama, Youvika Singh, Patricia Aoki Miyasato, Giulliana Tessarin Almeida, Dunia Rodriguez, Mayra Mara Ferrari Barbosa, Rafaela Sachetto Fernandes, Tereza Cristina Barbosa, Almiro Pires da Silva Neto, Eliana Nakano, Paulo Lee Ho, Sergio Verjovski-Almeida, Helder Imoto Nakaya, Robert Alan Wilson, Luciana Cezar de Cerqueira Leite
Summary: Despite decades of research, an effective vaccine against schistosomiasis is still elusive. The radiation-attenuated cercarial vaccine remains the best model for high protection levels, activating Th2 pathways and suppressing hemostasis mechanisms. Single exposure to attenuated parasites establishes a Th1 bias and enhances inflammation, while multiple doses do not significantly increase protection but show signs of cytotoxicity and apoptosis pathways. The limitation of a Th1 response may explain the failure to achieve sterile immunity despite multiple vaccinations.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Richard J. Mills, Sean J. Humphrey, Patrick R. J. Fortuna, Mary Lor, Simon R. Foster, Gregory A. Quaife-Ryan, Rebecca L. Johnston, Troy Dumenil, Cameron Bishop, Rajeev Rudraraju, Daniel J. Rawle, Thuy Le, Wei Zhao, Leo Lee, Charley Mackenzie-Kludas, Neda R. Mehdiabadi, Christopher Halliday, Dean Gilham, Li Fu, Stephen J. Nicholls, Jan Johansson, Michael Sweeney, Norman C. W. Wong, Ewelina Kulikowski, Kamil A. Sokolowski, Brian W. C. Tse, Lynn Devilee, Holly K. Voges, Liam T. Reynolds, Sophie Krumeich, Ellen Mathieson, Dad Abu-Bonsrah, Kathy Karavendzas, Brendan Griffen, Drew Titmarsh, David A. Elliott, James McMahon, Andreas Suhrbier, Kanta Subbarao, Enzo R. Porrello, Mark J. Smyth, Christian R. Engwerda, Kelli P. A. MacDonald, Tobias Bald, David E. James, James E. Hudson
Summary: Cardiac injury and dysfunction in COVID-19 patients increase mortality risk. An inflammatory cytokine-storm can induce diastolic dysfunction, but BET inhibitors show promise in preventing cardiac damage and dysfunction induced by the virus.
Article
Immunology
Tianhao Xu, Alexander Schutte, Leandro Jimenez, Andre N. A. Goncalves, Ashleigh Keller, Matthew E. Pipkin, Helder Nakaya, Renata M. Pereira, Gustavo J. Martinez
Summary: Kdm6b is essential for the proper generation and function of effector CD8(+) T cells, with its deficiency impacting effector cell generation and function, as well as recall response of memory CD8(+) T cells. The study suggests Kdm6b as a potential therapeutic target for diseases with dysregulated effector responses by regulating chromatin accessibility in effector-associated genes.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mateus Nobrega Aoki, Bruna de Oliveira Coelho, Luiz Gustavo Bentim Goes, Paola Minoprio, Edison Luiz Durigon, Luis Gustavo Morello, Fabricio Klerynton Marchini, Irina Natassja Riediger, Maria do Carmo Debur, Helder I. Nakaya, Lucas Blanes
Summary: RT-LAMP is a promising method for diagnosing COVID-19 with high sensitivity and specificity, showing a color change from pink to yellow in positive samples. The presence of an orange color cluster suggests the need for further validation in samples with low viral loads.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Igor Santiago-Carvalho, Gislane de Almeida-Santos, Caio Cesar Barbosa Bomfim, Paula Carolina de Souza, Juan Carlo Santos e Silva, Bruno Marcel Silva de Melo, Eduardo Pinheiro Amaral, Marcos Vinicios Pinheiro Cione, Elena Lasunskaia, Mario Hiroyuki Hirata, Jose Carlos Farias Alves-Filho, Helder Imoto Nakaya, Jose Maria Alvarez, Maria Regina D'Imperio Lima
Summary: A study found that pharmacological blockade of the P2X7 receptor can alleviate the severity of severe pulmonary tuberculosis, including reducing weight loss, slowing the development of inflammatory and necrotic lung lesions, and delaying mycobacterial growth. This treatment also reduces TNF-alpha production and improves immune responses, suggesting the potential of targeting the P2X7 receptor as a therapeutic strategy for pulmonary tuberculosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Shuxiong Chen, Benjamin Evert, Adetayo Adeniyi, Merce Salla-Martret, Linda H. -L. Lua, Victoria Ozberk, Manisha Pandey, Michael F. Good, Andreas Suhrbier, Peter Halfmann, Yoshihiro Kawaoka, Bernd H. A. Rehm
Summary: The biopolymer particle (BP) vaccine technology offers a promising solution to the unmet need for safe and effective SARS-CoV-2 vaccines that can be produced at large scale. These vaccines, utilizing coronavirus antigen-coated BPs, have shown safety and immunogenicity in mice and induced protective immunity in a hamster SARS-CoV-2 infection model. The BP platform enables rapid design and cost-effective manufacture of ambient temperature stable vaccines to combat the COVID-19 pandemic.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Immunology
Jessamine E. Hazlewood, Bing Tang, Kexin Yan, Daniel J. Rawle, Jessica J. Harrison, Roy A. Hall, Jody Hobson-Peters, Andreas Suhrbier
Summary: A chimeric flavivirus vaccine based on Binjari virus was developed and utilized to generate a chimeric ZIKV vaccine. Vaccination with the unadjuvanted chimeric vaccine induced long-lasting neutralizing antibody responses and provided full protection against ZIKV infection in mice.
Article
Biochemistry & Molecular Biology
Seth T. Kazmer, Gunter Hartel, Harley Robinson, Renee S. Richards, Kexin Yan, Sebastiaan J. van Hal, Raymond Chan, Andrew Hind, David Bradley, Fabian Zieschang, Daniel J. Rawle, Thuy T. Le, David W. Reid, Andreas Suhrbier, Michelle M. Hill
Summary: This study investigates the biochemical changes and potential diagnostic use of Fourier transform infrared (FTIR) spectroscopy in COVID-19 screening. It demonstrates that SARS-CoV-2 infection induces significant changes in culture supernatant and oral lavage samples, including aggregated proteins and RNA. The study also develops a predictive model for COVID-19 using saliva samples and highlights alterations in beta-sheet structures in secreted proteins as a common response to SARS-CoV-2 infection.
Article
Multidisciplinary Sciences
Thomaz Luscher-Dias, Rodrigo Juliani Siqueira Dalmolin, Paulo de Paiva Amaral, Tiago Lubiana Alves, Viviane Schuch, Gloria Regina Franco, Helder I. Nakaya
Summary: This study used cognitive computing text-mining techniques to construct a knowledge network of genes and diseases and analyzed the changes in these networks over the past 30 years. The study revealed the molecular basis of diseases, shared mechanisms between different diseases, and the genes commonly associated with multiple disorders. By exploring this knowledge network, relevant biological information and insights about human diseases can be extracted.
Article
Multidisciplinary Sciences
Corentin Mallet, Laurent Souci, Mireille Ledevin, Sonia Georgeault, Thibaut Larcher, Caroline Denesvre
Summary: Protocols for in vitro culture of human hair follicles were developed 30 years ago, and now a method for the in vitro culture of chicken feather follicles has been successfully developed. The study shows that this method can maintain the structure and biology of feather follicles close to their in vivo state, despite transcriptional modifications of some genes involved in feather development. This new culture model can be used to study interactions between feathers and pathogens or toxins, and reduce animal experimentation.
Article
Health Care Sciences & Services
Michelle G. Discacciati, Sirlei Siani, Ana Campa, Helder Nakaya
Summary: Obesity alone is a greater risk factor for in-hospital mortality than cardiovascular disease and diabetes. Additionally, obesity, cardiovascular disease, and diabetes increase the risk of severe COVID-19 and death more significantly in young adults than in the elderly.
LANCET REGIONAL HEALTH-AMERICAS
(2022)