Article
Virology
Kerry Dust, Michael Carpenter, Julie Chih-yu Chen, Chris Grant, Stuart McCorrister, Garret R. Westmacott, Alberto Severini
Summary: This study investigates the effects of HPV16 viral proteins E6 and E7 on the host proteome in human neonatal keratinocytes. The results show that the expression of E6 and E7 leads to dysregulation in pathways related to interferon response, inflammation, and DNA damage repair. The study also identifies potential biomarkers associated with HPV oncoproteins in primary keratinocytes.
Article
Genetics & Heredity
Lindsey D. Goodman, Heidi Cope, Zelha Nil, Thomas A. Ravenscroft, Wu-Lin Charng, Shenzhao Lu, An-Chi Tien, Rolph Pfundt, David A. Koolen, Charlotte A. Haaxma, Hermine E. Veenstra-Knol, Jolien S. Klein Wassink-Ruiter, Marijke R. Wevers, Melissa Jones, Laurence E. Walsh, Victoria H. Klee, Miel Theunis, Eric Legius, Dora Steel, Katy E. S. Barwick, Manju A. Kurian, Shekeeb S. Mohammad, Russell C. Dale, Paulien A. Terhal, Ellen van Binsbergen, Brian Kirmse, Bethany Robinette, Benjamin Cogne, Bertrand Isidor, Theresa A. Grebe, Peggy Kulch, Bryan E. Hainline, Katherine Sapp, Eva Morava, Eric W. Klee, Erica L. Macke, Pamela Trapane, Christopher Spencer, Yue Si, Amber Begtrup, Matthew J. Moulton, Debdeep Dutta, Oguz Kanca, Michael F. Wangler, Shinya Yamamoto, Hugo J. Bellen, Queenie K-G Tan
Summary: The Drosophila gene dTnpo, orthologous to TNPO2, is essential for neuronal maintenance and function, with downregulation in mature neurons disrupting activity and survival. Mutant alleles or downregulation of dTnpo causes developmental defects, while upregulation results in similar phenotypes as loss of Transportin activity. Proband-associated variants in TNPO2 disrupt protein function, correlating to their location within the protein. Overall, TNPO2 variants are causative for neurodevelopmental abnormalities.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Biology
Joshua Hatterschide, Paola Castagnino, Hee Won Kim, Steven M. Sperry, Kathleen T. Montone, Devraj Basu, Elizabeth A. White
Summary: This study found that the HPV oncoprotein E7 activates YAP1 and promotes its nuclear localization in basal epithelial cells. E7 activates YAP1 through binding and degradation of PTPN14, which plays a critical role in HPV persistence and carcinogenesis.
Article
Virology
Shuo Li, Mengxia Ye, Yonglin Chen, Quan Gong, Bing Mei
Summary: This study sheds light on the relationship between HPV-52 and cervical cancer, identifying variations in the E6-E7 genes and analyzing the protein's structure and selective pressure. Multiple single nucleotide variants were found in the E6 and E7 sequences of HPV-52, some impacting B cell epitopes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Ji Young Song, Young Min Park, Soon Yong Choi
Summary: The study found that HPV-2 can significantly decrease the expression levels of E-cadherin, which affects cell adhesion and may contribute to the formation of cutaneous warts.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Virology
Sadhana M. Gupta, Himangi Warke, Hemangi Chaudhari, Padmaja Mavani, Rajshree D. Katke, Shilpa C. Kerkar, Jayanti Mania-Pramanik
Summary: This study aimed to evaluate HPV E6/E7 oncogene expression as markers for early identification of cervical cancer risk in women with minor cytological abnormalities and negative cytology. The results showed that E6 and E7 transcripts could serve as sensitive early predictors of cervical cancer risk.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Mengxia Ye, Shuo Li, Ping Luo, Xuan Tang, Quan Gong, Bing Mei
Summary: This study analyzed the variations and phylogenetic relationship of HPV-51 virus in central China, as well as predicted its immune epitopes. The results showed high prevalence and genetic variations of HPV-51 in the region. These findings are of great importance for future research on vaccines with wider coverage and the development of diagnostic probes.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Oncology
Jiang Zhu, Lingjing Chen, Qiaoli Zheng, Rui Han, Xianzhen Chen, Qiang Zhou, Hao Cheng
Summary: This study aimed to establish a reliable protocol for immunohistochemistry stains to detect HPV16 E7 protein and found a positive correlation between HPV16 E7 protein expression and malignancy grade.
Review
Virology
James C. Romero-Masters, Paul F. Lambert, Karl Munger
Summary: HPVs cause a wide range of human diseases, from benign warts to malignant cancers. The discovery of a murine papillomavirus that infects laboratory mice provides an opportunity to study HPV-induced malignant diseases. The E6 and E7 proteins encoded by high-risk HPVs contribute to HPV-induced cancers in multiple ways.
Article
Microbiology
James C. Romero-Masters, Miranda Grace, Denis Lee, Joshua Lei, Melanie DePamphilis, Darya Buehler, Rong Hu, Ella Ward-Shaw, Simon Blaine-Sauer, Nathalie Lavoie, Elizabeth A. White, Karl Munger, Paul F. Lambert
Summary: Human papillomaviruses (HPVs) contribute to 5% of human cancers. Murine papillomavirus (MmuPV1) provides a powerful tool to study the roles of papillomavirus genes in pathogenesis arising from a natural infection. In this study, we confirmed the interaction between MmuPV1 E7 and PTPN14, and found that the intact PTPN14 binding site is essential for MmuPV1 E7 to promote virus-induced pathogenesis and delay epithelial differentiation.
Article
Medicine, Research & Experimental
Diane Bruyere, Patrick Roncarati, Alizee Lebeau, Thomas Lerho, Florian Poulain, Elodie Hendrick, Charlotte Pilard, Celia Reynders, Marie Ancion, Margaux Luyckx, Michael Renard, Yves Jacob, Jean-Claude Twizere, Raphael Peiffer, Olivier Peulen, Philippe Delvenne, Pascale Hubert, Alison McBride, Nicolas Gillet, Murielle Masson, Michael Herfs
Summary: HPV-positive cancers are usually associated with a favorable outcome due to high sensitivity to radiation therapy. However, the impact of viral E6/E7 oncoproteins on cellular radiosensitivity and host DNA repair is still largely speculative.
Article
Cell Biology
Lingfang Wang, Guankai Zhan, Yasen Maimaitiyiming, Yingfeng Su, Shitong Lin, Jinfeng Liu, Kunhui Su, Jiebo Lin, Shizhen Shen, Wentao He, Fenfen Wang, Jiafeng Chen, Siqi Sun, Yite Xue, Jiaxin Gu, Xiaojing Chen, Jian Zhang, Lu Zhang, Qianqian Wang, Kao-Jung Chang, Shih-Hwa Chiou, Mikael Bjorklund, Hua Naranmandura, Xiaodong Cheng, Chih-Hung Hsu
Summary: This study reveals that heat treatment can selectively target the messenger RNA (mRNA) containing the E7 onco-transcript complex of human papillomavirus (HPV). By destabilizing the cellular reader protein IGF2BP1, heat treatment downregulates E7 mRNA and protein expression, effectively reversing HPV-induced carcinogenesis. Mechanistically, heat treatment promotes the aggregation of IGF2BP1 only in the presence of m6A-modified E7 mRNA, which is subsequently resolved by the ubiquitin-proteasome system. These findings provide a novel heat treatment-based therapeutic strategy for HPV-associated malignancies.
Article
Biochemistry & Molecular Biology
Hye Seon Lee, Min Wook Kim, Kyeong Sik Jin, Ho-Chul Shin, Won Kon Kim, Sang Chul Lee, Seung Jun Kim, Eun-Woo Lee, Bonsu Ku
Summary: The interactions of the viral oncoprotein E7 with PTPN14 and PTPN21 are similar at the molecular level but play different physiological roles. PTPN21 exhibits pro-tumor activity and shows differences in biological consequences compared to PTPN14.
MOLECULES AND CELLS
(2021)
Article
Biochemistry & Molecular Biology
Ji Young Song, Ju Hee Han, Yumee Song, Ji Hyun Lee, Soon Yong Choi, Young Min Park
Summary: The study found that HPV-2 infection significantly reduces the expression of all ISGs in cutaneous warts, and E7 transfection further downregulates the expression of these genes, but EGCG pretreatment can reverse this trend, showing potential for cutaneous wart prevention.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Ramon Antano-Arias, Oscar Del Moral-Hernandez, Julio Ortiz-Ortiz, Luz Del Carmen Alarcon-Romero, Jorge Adan Navor-Hernandez, Marco Antonio Leyva-Vazquez, Marco Antonio Jimenez-Lopez, Jorge Organista-Nava, Berenice Illades-Aguiar
Summary: This study identified specific E7 variants associated with the highest risk of cervical cancer, with E6/E7 bicistron analysis revealing even greater risk potential for specific variants. These findings underscore the importance of analyzing combinations of E6/E7 variants in HPV 16 infection for predicting cervical cancer development.
Article
Otorhinolaryngology
Hugh A. J. Kim, Peter Y. F. Zeng, Alana Sorgini, Mushfiq H. Shaikh, Halema Khan, Danielle MacNeil, Mohammed Khan, Adrian Mendez, John Yoo, Kevin Fung, Pencilla Lang, David A. Palma, Joe S. Mymryk, John W. Barrett, Krupal B. Patel, Paul C. Boutros, Anthony C. Nichols
Summary: Numerous studies have shown that head and neck squamous cell carcinoma (HNSCC) outcomes differ among different races and ethnicities. This study found that Black patients with non-HPV-related tumors had poorer progression-free survival compared to White patients. The tumors of Black patients exhibited molecular differences, including copy number aberrations, methylation of SFRP1, and miRNA-mediated PRG4 silencing, which were associated with poor survival. These findings can help inform targeted treatment decisions to achieve equitable outcomes.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2022)
Article
Otorhinolaryngology
M. Wojtera, J. W. Barrett, J. E. Strychowsky, A. Dzioba, J. Paradis, M. Husein, M. Salvadori, A. C. Nichols
Summary: No human papillomavirus subtypes 6, 11, 16 or 18 were found in paediatric tonsil specimens from Southwestern Ontario.
JOURNAL OF LARYNGOLOGY AND OTOLOGY
(2022)
Article
Otorhinolaryngology
Nicole Pinto, Kara M. Ruicci, Mohammed Imran Khan, Mushfiq Hassan Shaikh, Yu Fan Peter Zeng, John Yoo, Kevin Fung, S. Danielle MacNeil, Adrian Mendez, Joe S. Mymryk, John W. Barrett, Paul C. Boutros, Anthony C. Nichols
Summary: This study provides evidence of the role of PIK3CA(E545K) in driving disease progression from a well-differentiated to an undifferentiated thyroid cancer, but over-expression is not a determinant of an accelerated growth phenotype in ATC.
JOURNAL OF OTOLARYNGOLOGY-HEAD & NECK SURGERY
(2022)
Review
Oncology
Adrian Mendez, Jacob G. J. Wihlidal, Dean T. Eurich, Anthony C. Nichols, S. Danielle MacNeil, Hadi R. Seikaly
Summary: Malignancy and treatment effects in head and neck oncology can have devastating impacts on functional aspects of patient life, such as swallowing, speech, dry mouth, and chewing. Patient-Reported Outcomes (PROs) have become the gold standard for assessing subjective functional outcomes, but few have incorporated extensive patient input in their development process. More efforts are needed to involve head and neck cancer patients in PRO development, particularly in the domains of speech and chewing.
Article
Dentistry, Oral Surgery & Medicine
Axel Sahovaler, Hubert Low, Francisco Laxague, Eun-Jae Chung, Kevin Fung, S. Danielle Mac Neil, Anthony C. Nichols, John Yoo
Summary: The study reviewed 40 patients who underwent scapular tip free flap reconstruction and found significant variations in the subscapular vascular anatomy, which have important implications for surgical access and patient positioning.
ORAL AND MAXILLOFACIAL SURGERY-HEIDELBERG
(2022)
Article
Otorhinolaryngology
K. B. Patel, S. Prokopec, J. W. Barrett, J. S. Mymryk, P. C. Boutros, A. C. Nichols
Summary: This study aimed to establish early passage cell lines with matched normal reference DNA for precision medicine in drug response evaluation. It successfully established early passage cell lines and confirmed their association with the primary tumor through short tandem repeat profiling. The study highlights the importance of matched normal reference DNA for accurate mutational analysis in cell lines.
JOURNAL OF LARYNGOLOGY AND OTOLOGY
(2023)
Article
Medicine, Research & Experimental
Francisco Laxague, Agnieszka Dzioba, Dhruv Jasani, Andrew Warner, David A. Palma, Pencilla Lang, Varagur Venkatesan, Nancy Read, Anthony C. Nichols, Kevin Fung, John Yoo, Adrian Mendez, Danielle MacNeil
Summary: This study analyzed risk factors associated with poor survival outcomes for metastatic cutaneous head-and-neck squamous cell carcinoma (SCC) to the parotid gland. The results showed that a shorter duration of adjuvant radiation therapy (RT) and excised tumor volume >= 50 cm(3) were predictive factors of reduced overall survival (OS) and disease-specific survival (DSS). A shorter duration of RT was also associated with reduced freedom from locoregional recurrence (LRR).
Article
Otorhinolaryngology
James Fowler, Yael Campanile, Andrew Warner, Francisco Laxague, Naif Fnais, Kevin Fung, Adrian Mendez, Danielle MacNeil, John Yoo, David Palma, Anthony Nichols
Summary: The study found that in head and neck surgery, patients with margins greater than 1 mm had similar local recurrence and overall survival rates compared to patients with margins greater than 5 mm, while patients with margins less than 1 mm showed worse survival outcomes.
JOURNAL OF OTOLARYNGOLOGY-HEAD & NECK SURGERY
(2022)
Letter
Oncology
David A. Palma, Anthony C. Nichols
Article
Otorhinolaryngology
Jacob G. J. Wihlidal, Keng Yeow Tay, S. Danielle MacNeil, Anthony C. Nichols, Kevin Fung, John H. J. Yoo, Adrian Mendez
Summary: The objective of this study was to quantify the growth rate of p16-negative oral cavity squamous cell carcinoma, explore the causative relationships between demographic risk factors and tumor growth, and examine pathologic findings associated with the tumor growth rate. Results from a small prospective cohort sample suggest clinical associations between alcohol consumption, origination at the retromolar trigone, and clinical nodal stage with rate of tumor growth.
LARYNGOSCOPE INVESTIGATIVE OTOLARYNGOLOGY
(2022)
Article
Oncology
Philipp Guevorguian, Tricia Chinnery, Pencilla Lang, Anthony Nichols, Sarah A. Mattonen
Summary: This study validated a radiomics model for risk stratification in oropharyngeal cancer, which showed comparable performance to the clinical model and outperformed when combined with clinical data. Additionally, a model for detecting dental artifacts on computed tomography images was also validated.
RADIOTHERAPY AND ONCOLOGY
(2023)
Article
Otorhinolaryngology
Nauman H. Malik, Rui Fu, Nicolin Hainc, Christopher W. Noel, John R. de Almeida, Ali Hosni, Shao Hui Huang, Eugene Yu, Agnieszka Dzioba, Andrew Leung, Arvindpaul Mangat, Danielle MacNeil, Anthony C. Nichols, Shivaprakash B. Hiremath, Santanu Chakraborty, Alboorz Jooya, Marc Gaudet, Stephanie Johnson-Obaseki, Jonathan Whelan, Reza Forghani, Michael P. Hier, Gregoire Morand, Khalil Sultanem, Joseph Dort, John Lysack, Wayne Matthews, Steven Nakoneshny, Gia Gill, Adam Globerman, Paul Kerr, Pejman Maralani, Irene Karam, Antoine Eskander
Summary: This study aimed to evaluate the association between primary tumor volume and oncologic outcomes in patients with T3 glottic cancer treated with (chemo)radiotherapy. The study found that increasing tumor volume was associated with worse overall survival and disease-free survival in these patients.
JAMA OTOLARYNGOLOGY-HEAD & NECK SURGERY
(2023)
Review
Oncology
Hugh Andrew Jinwook Kim, Anthony Charles Nichols, Ramanamurthy Rachakonda, Richard Inculet, Jinka Sathya, Irina Rachinsky, Eric Winquist
Summary: This article reports a case of unresectable or metastatic differentiated thyroid carcinoma treated with lenvatinib and external beam radiation therapy. The patient showed clinical, biochemical, and radiological response after 5 months of lenvatinib and subsequently received external beam radiation. 21 months post-initiation of treatment, the patient enjoys good quality of life without evidence of cancer progression.
Article
Oncology
Anthony C. Nichols, Julie Theurer, Eitan Prisman, Nancy Read, Eric Berthelet, Eric Tran, Kevin Fung, John R. de Almeida, Andrew Bayley, David P. Goldstein, Michael Hier, Khalil Sultanem, Keith Richardson, Alex Mlynarek, Suren Krishnan, Hien Le, John Yoo, S. Danielle MacNeil, Eric Winquist, J. Alex Hammond, Varagur Venkatesan, Sara Kuruvilla, Andrew Warner, Sylvia Mitchell, Jeff Chen, Martin Corsten, Stephanie Johnson-Obaseki, Michael Odell, Christina Parker, Bret Wehrli, Keith Kwan, David A. Palma
Summary: The study found that for early-stage OPSCC, radiotherapy performed better in terms of long-term swallowing quality of life compared to transoral robotic surgery plus neck dissection, although this difference decreases over time. Some differences in QOL concerns gradually resolved in the long term, but it is worth noting that TORS patients started using more nutritional supplements at 3 years, and dry mouth scores increased over time in RT patients.
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Review
Health Policy & Services
Sepehr Shabani, Kea Turner, Anthony C. Nichols, Xuefeng Wang, Krupal B. Patel
Summary: This article discusses the health care disparities in head and neck squamous cell carcinomas, particularly focusing on racial differences. It highlights that African American patients are diagnosed at a younger age, present with higher tumor burden, receive less definitive cancer treatment, and have increased mortality compared to non-African American patients. The article suggests that these disparities can be eliminated through education, screening programs, and expanding health care coverage.
JOURNAL OF HEALTH CARE FOR THE POOR AND UNDERSERVED
(2022)