期刊
JOURNAL OF VIROLOGY
卷 87, 期 23, 页码 12667-12674出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.02261-13
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资金
- BIOTEC Young Fellow Research Grant [P-10-10246]
- Platform Technology Grant from BIOTEC, Thailand [P-12-01007]
- Medical Research Council, United Kingdom
- Wellcome Trust, United Kingdom
- National Institute for Health Research Biomedical Research Centre
- Office of the Higher Education Commission and Mahidol University under the National Research Universities Initiative
- European Commission [282 378]
- MRC [G0801508, G0600000, G0400720] Funding Source: UKRI
- Medical Research Council [G0801508, G0400720] Funding Source: researchfish
Here we present an approach that advances the throughput of a genetic analysis of a positive-sense RNA virus by simplifying virus construction. It enabled comprehensive dissection of a complex, multigene phenotype through rapid derivation of a large number of chimeric viruses and construction of a mutant library directly from a virus pool. The versatility of the approach described here expands the applicability of diverse genetic approaches to study these viruses.
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