Article
Plant Sciences
Jiacai Chen, Xinying Sui, Binran Ma, Yuetong Li, Na Li, Longfei Qiao, Yanchong Yu, Chun-Hai Dong
Summary: This study elucidates the regulatory mechanism of Arabidopsis CPR5 in ethylene signaling, including its interaction with the ETR1 receptor and its control over the nucleocytoplasmic transport of ethylene-related mRNAs. These findings have significant implications for our understanding of the role of CPR5 in ethylene signaling.
PLANT CELL REPORTS
(2022)
Article
Plant Sciences
Yuanyuan Nie, Yang Li, Menghui Liu, Binran Ma, Xinying Sui, Jiacai Chen, Yanchong Yu, Chun-Hai Dong
Summary: This study reveals the involvement of Arabidopsis nucleoporins NUP160 and NUP96 in nucleocytoplasmic transport of mRNAs related to ethylene signaling pathway, advancing our understanding of the regulatory mechanisms of nucleoporin proteins in ethylene signaling and response.
PLANT CELL REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Julie Kubina, Angele Geldreich, Jon Pol Gales, Nicolas Baumberger, Clement Bouton, Lyubov A. Ryabova, Klaus D. Grasser, Mario Keller, Maria Dimitrova
Summary: The study shows that the lack of output factors such as ALY, MOS11, and/or TEX1 in plants can inhibit the export of viral mRNA, thereby increasing the resistance of plants to CaMV infection.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Virology
Margarita Valdes Aleman, Luca D. Bertzbach, Thomas Speiseder, Wing Hang Ip, Ramon A. Gonzalez, Thomas Dobner
Summary: During human adenovirus infection, the late phase of viral replication results in a significant increase in viral late mRNA abundance, which outcompetes cellular mRNA biogenesis rather than selectively exporting viral mRNA.
Article
Agronomy
Qiufei Peng, Jieyu Qiu, Xintong Li, Xuezhong Xu, Xinxiang Peng, Guohui Zhu
Summary: Transportation of proteins to and from the nucleus is essential for cellular processes and plant responses to signals and stresses. The nuclear export receptor OsXPO1 was identified in rice and found to play a role in regulating plant development and responses to abiotic stress. Loss-of-function mutations of OsXPO1 resulted in lethal seedlings, while its suppression led to various developmental defects. Overexpression of OsXPO1 reduced plant height but increased tolerance to drought and salt stress in rice.
Article
Virology
Kuan Feng, Hui-Jiao Zhang, Yuan-Qin Min, Min Zhou, Fei Deng, Hua-Lin Wang, Pei-Qing Li, Yun-Jia Ning
Summary: COVID-19, caused by SARS-CoV-2, poses an unprecedented threat to human health. The progression of the disease is associated with impaired antiviral IFN responses. This study demonstrates that SARS-CoV-2 NSP13 protein antagonizes IFN response by targeting IRF3, independently of TBK1. The strong interaction between NSP13 and IRF3 suggests that IRF3 is a major target for NSP13 in immune evasion.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Cell Biology
William Dunker, Xiang Ye, Yang Zhao, Lanxi Liu, Antiana Richardson, John Karijolich
Summary: TDP-43 is shown to regulate the accumulation of immunostimulatory dsRNA, preventing interferon-mediated cell death. This study reveals an intricate relationship between the control of cellular gene expression and IFN-mediated cell death.
Article
Multidisciplinary Sciences
Alessandra Gurtner, Costanza Borrelli, Ignacio Gonzalez-Perez, Karsten Bach, Ilhan E. Acar, Nicolas G. Nunez, Daniel Crepaz, Kristina Handler, Vivian P. Vu, Atefeh Lafzi, Kristin Stirm, Deeksha Raju, Julia Gschwend, Konrad Basler, Christoph Schneider, Emma Slack, Tomas Valenta, Burkhard Becher, Philippe Krebs, Andreas E. Moor, Isabelle C. Arnold
Summary: In the past decade, single-cell transcriptomics has provided insights into the biology of eosinophils, a difficult-to-sequence cell type, and their roles in intestinal homeostasis, immune regulation, and host defense. The study reveals the heterogeneity of eosinophils and the gene programs that underpin their pleiotropic functions. It also explains the mechanism by which interleukin-33 (IL-33) and interferon-gamma (IFN gamma) induce the accumulation of active eosinophils in the inflamed colon and their association with CD4(+) T cells.
Article
Biotechnology & Applied Microbiology
Xiaoyan Zhao, Yan Sun, Ziwei Xu, Li Cai, Yu Hu, Huafang Wang
Summary: Protein arginine methyl-transferase 1 (PRMT1) plays a critical role in graft-versus-host disease (GVHD). Inhibiting PRMT1 can prevent chronic and acute GVHD by reducing the percentage of Th17 cells and B cells, as well as controlling cell proliferation and antibody production. PRMT1 regulates B cell function by methylating isocitrate dehydrogenase 2 (IDH2).
Article
Immunology
Wan Li, Zhengnan Cai, Florian Schindler, Sheyda Bahiraii, Martin Brenner, Elke H. Heiss, Wolfram Weckwerth
Summary: This study aimed to investigate the preventive effects of norbergenin on LPS-induced proinflammatory signaling in macrophages and elucidate the underlying mechanisms. It was found that norbergenin alleviated LPS-induced production of inflammatory factors in macrophages by suppressing the activation of TLR2 mediated signaling pathways. Furthermore, norbergenin regulated metabolic reprogramming in LPS-stimulated macrophages, promoting OXPHOS and restoring aberrant metabolites within the TCA cycle.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Plant Sciences
Xinmin Lv, Yaqiang Sun, Pengbo Hao, Cankui Zhang, Ji Tian, Mengmeng Fu, Zhen Xu, Yi Wang, Xinzhong Zhang, Xuefeng Xu, Ting Wu, Zhenhai Han
Summary: This study reveals that mRNA mobility differs between herbaceous and woody plants, with apple mRNA able to be transported over long distances while Arabidopsis mRNA cannot. The divergent transmissibility of OPT3 in Arabidopsis and Malus may be caused by differences in RNA-binding proteins between herbaceous and woody plants.
Article
Medicine, Research & Experimental
Akshita Gupta, Angelina Konnova, Mathias Smet, Matilda Berkell, Alessia Savoldi, Matteo Morra, Vincent Van Averbeke, Fien H. R. De Winter, Denise Peserico, Elisa Danese, An Hotterbeekx, Elda Righi, Pasquale De Nardo, Evelina Tacconelli, Surbhi Malhotra-Kumar, Samir Kumar-Singh
Summary: In this study, researchers observed the role of host immunity in the emergence of SARS-CoV-2 evasive Spike mutations under therapeutic monoclonal antibody pressure. The results showed that different monoclonal antibody treatments led to the development of mutations, and immunocompromised patients were more likely to develop mutations. The study also found that the neutralizing capacity of the antibodies and T cell immunity were closely related to the development of mutations, and an anti-inflammatory and healing-promoting host environment facilitated mutation development. These data provide evidence for decision-making to reduce the risk of monoclonal antibody treatment failure and improve strategies to mitigate the spread of escape mutants.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Microbiology
Simona Seizova, Ushma Ruparel, Alexandra L. Garnham, Stefanie M. Bader, Alessandro D. Uboldi, Michael J. Coffey, Lachlan W. Whitehead, Kelly L. Rogers, Christopher J. Tonkin
Summary: This study reveals that bradyzoites of Toxoplasma gondii alter the gene expression of infected host cells by exporting specific proteins, leading to suppression of interferon signaling. The exported protein IST plays a critical role in limiting interferon signaling. Furthermore, bradyzoite protein export protects host cells from interferon-induced cell death.
CELL HOST & MICROBE
(2022)
Article
Cell Biology
Haiyang Sun, Ce Tang, Soo-Hyun Chung, Xiao-Qi Ye, Yulia Makusheva, Wei Han, Masato Kubo, Shigeyuki Shichino, Satoshi Ueha, Koji Matsushima, Kazuho Ikeo, Masahide Asano, Yoichiro Iwakura
Summary: DCIR plays important roles in regulating the immune and bone systems, but its role in the intestine is unclear. Blocking DCIR signaling can improve colitis and suppress the development of colonic tumors, indicating that DCIR may be a potential target for the treatment of these diseases.
Article
Biochemistry & Molecular Biology
Mahrukh Naudhani, Kiran Thakur, Zhi-Jing Ni, Jian-Guo Zhang, Zhao-Jun Wei
Summary: The study demonstrates that formononetin (FMNT) can effectively improve obesity-related metabolic disorders, regulate gut microbiota, suppress inflammation, and increase the production of acetate, lactate, and butyrate at specific dosage.
Article
Virology
Nayeli Aguilar-Hernandez, Lena Meyer, Susana Lopez, Rebecca M. DuBois, Carlos F. Arias
Summary: In this study, the researchers identified PDIA4 as a binding partner for HAstV-1 and -8 spikes, with the PDI inhibitor 16F16 strongly inhibiting infection by HAstV serotypes 1 and 8. It was also shown that RNA interference of PDIA4 expression selectively blocked HAstV-8 infectivity. These findings suggest that PDIA4 is essential for the uncoating of the viral genome during the cell entry process of HAstV-1 and -8.
Article
Infectious Diseases
Sandra Gomez-Fuentes, Sarah Hernandez-de la Fuente, Valeria Morales-Ruiz, Dina Lopez-Recinos, Adrian Guevara-Salinas, Maria Cristina Parada-Colin, Clara Espitia, Adrian Ochoa-Leyva, Filiberto Sanchez, Nelly Villalobos, Asiel Arce-Sillas, Marisela Hernandez, Silvia Ivonne Mora, Gladis Fragoso, Edda Sciutto, Laura Adalid-Peralta
Summary: Human and pig cysticercosis caused by the flatworm Taenia solium can lead to neurocysticercosis, a potentially fatal disease. This study aimed to identify and characterize cysticercal secretion proteins using 2D-PAGE proteomic analysis, resulting in the identification of potential proteins for designing vaccines or diagnostic methods for neurocysticercosis. The study also introduced a novel strategy to predict the sequence of T. solium secreted proteins, which could have applications in diagnosis and prevention of the disease.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Virology
Lena Ricemeyer, Nayeli Aguilar-Hernandez, Tomas Lopez, Rafaela Espinosa, Sarah Lanning, Santanu Mukherjee, Carolina Cuellar, Susana Lopez, Carlos F. Arias, Rebecca M. DuBois
Summary: Human astrovirus is a major cause of viral gastroenteritis worldwide, and this study provides a basis for developing an effective vaccine to prevent and treat human astrovirus infection by understanding how antibodies block the virus at the molecular level.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Jing-Hsiung James Ou, Richard J. Kuhn, Susana Lopez, Charles M. Rice
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Jose L. Martinez, Catherine Eichwald, Elisabeth M. Schraner, Susana Lopez, Carlos F. Arias
Summary: Lipid metabolism is crucial for rotavirus replication, not only in viroplasm assembly but also in maintaining infectious virus production and membrane synthesis.
Article
Virology
Catalina Aguilera-Flores, Tomas Lopez, Fernando Zamudio, Carlos Sandoval-Jaime, Edmundo Perez, Susana Lopez, Rebecca DuBois, Carlos F. Arias
Summary: Human astrovirus VA1, associated with neurological disease in immunocompromised patients, can replicate in cell culture without trypsin. The capsid precursor protein of astrovirus VA1 undergoes intracellular cleavage to form mature infectious particles, composed of two polypeptides, VP33 and VP38. These studies provide insight into astrovirus VA1 cell entry and replication.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Carlos Baez-Navarro, Ivan R. Quevedo, Susana Lopez, Carlos F. Arias, Pavel Isa
Summary: Astroviruses are a significant cause of diarrhea, especially in vulnerable populations like children. Recent research has found these viruses in organs outside the intestines, leading to unexpected clinical diseases. This study discovered that human astrovirus strain Yuc8 is associated with extracellular vesicles, potentially during or after their release from cells. This association increases the infectivity of the virus and protects it from neutralization by antibodies, indicating that extracellular vesicles could be a novel pathway for astrovirus dissemination.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Joaquin Moreno-Contreras, Liliana Sanchez-Tacuba, Carlos F. Arias, Susana Lopez
Summary: Rotaviruses have a genome composed of 11 segments of double-stranded RNA. A study found that there were similar proportions of capped and noncapped viral RNAs in rotavirus-infected cells and viral particles.
JOURNAL OF VIROLOGY
(2022)
Article
Veterinary Sciences
Pavel Isa, Luis Felipe Paulin, Jesus Hernandez, Maricela Montalvo-Corral, Susana Lopez, Carlos F. Arias
Summary: Genetic characterization of field samples from Mexican wetlands revealed the genomics and virus circulation of influenza A viruses, highlighting the importance of further studies in this region.
VETERINARIA MEXICO
(2022)
Article
Virology
Matthew Ykema, Kai Ye, Meng Xun, Justin Harper, Miguel A. A. Betancourt-Solis, Carlos F. F. Arias, James A. A. McNew, Yizhi Jane Tao
Summary: The human astrovirus (HAstV) is a common cause of gastroenteritis, and its cell entry mechanism and membrane penetration activity have been investigated in this study. It was found that the capsid protein of HAstV has a trypsin-dependent lipid disruption activity, essential for virus infectivity. This discovery provides valuable insights into HAstV pathogenesis and offers a potential target for therapeutics.
JOURNAL OF VIROLOGY
(2023)
Article
Infectious Diseases
Adriana Becerra, Pavel Isa, Ana Lorena Gutierrez-Escolano, Federico Raul Velazquez, Javier Torres, Carlos Federico Arias, Teresa Estrada-Garcia
Summary: Diarrhoeal illness is a major cause of morbidity/mortality in children from less-developed regions. This study investigated the gut microbiome of children with diarrhoea, focusing on the virome. The results showed inter-individual viral species composition in the stools of diarrhoeal children, with bacteriophages being the most abundant group. Children under 2 years of age had significantly higher viral richness compared to older children, primarily due to bacteriophages and diarrheagenic-viral species. Stool samples preserved at -70 degrees C can be successfully used for microbiome studies.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2023)
Article
Microbiology
Xaira Rivera-Gutierrez, Patricia Moran, Blanca Taboada, Angelica Serrano-Vazquez, Pavel Isa, Liliana Rojas-Velazquez, Horacio Perez-Juarez, Susana Lopez, Javier Torres, Cecilia Ximenez, Carlos F. Arias
Summary: A metagenomic study in a semirural community in Mexico revealed a high prevalence of norovirus and sapovirus in infants' fecal samples, with no symptomatic cases observed. The study highlights the importance of metagenomic analysis in understanding the diversity and prevalence of gastrointestinal viruses in early life.
MICROBIOLOGY SPECTRUM
(2022)