Article
Virology
Emma K. Loveday, Kyle S. Hain, Irina Kochetkova, Jodi F. Hedges, Amanda Robison, Deann T. Snyder, Susan K. Brumfield, Mark J. Young, Mark A. Jutila, Connie B. Chang, Matthew P. Taylor
Summary: Research has shown that heat treatment for 15 minutes at 65 degrees Celsius or UV irradiation at 70,000 mu J/cm(2) can completely inactivate SARS-CoV-2, offering efficient options for research purposes. It has also been observed that UV irradiation results in a 2-log reduction of detectable viral genomes compared to heat inactivation, indicating differences in effectiveness between the two methods.
Article
Ophthalmology
Charles G. Miller, Matthew Henderson, Dimosthenis Mantopoulos, Ilya Leskov, Todd Greco, Jean E. Schwarzbauer, Jonathan L. Prenner
Summary: This study identified two enriched pathways in PVR: extracellular matrix (ECM) organization and extracellular structure organization. A fold-change analysis revealed fibronectin as the protein most significantly elevated in PVR compared to ERM. These findings indicate key pathways involved in PVR progression and cell-mediated ECM assembly.
OPHTHALMIC SURGERY LASERS & IMAGING RETINA
(2021)
Article
Microbiology
Timothy R. Howard, Marni S. Crow, Todd M. Greco, Krystal K. Lum, Tuo Li, Ileana M. Cristea
Summary: The research indicates that IFIX plays a significant role in impacting both nuclear and cytoplasmic proteomes, leading to alterations in immune signaling, DNA damage response, and vesicle-mediated transport proteins. Additionally, IFIX is shown to have multifunctional roles in immune signaling, DNA damage response, and transcriptional regulation within the host cell.
Article
Biology
Debora Parrine, Todd M. Greco, Bilal Muhammad, Bo-Sen Wu, Xin Zhao, Mark Lefsrud
Summary: The study reveals different responses of plants to extreme high-light stress under blue light and red light. Blue light stimulates a more efficient energy dissipation mechanism, while red light increases the abundance of photosystem II and oxygen-evolving complex proteins. The impact of extreme high-light stress on the proteomic level is wavelength-dependent, with different wavelengths leading to varied responses in plants.
Article
Cell Biology
Sheeja Navakkode, Jessica Ruth Gaunt, Maria Vazquez Pavon, Vibhavari Aysha Bansal, Riya Prasad Abraham, Yee Song Chong, Toh Hean Ch'ng, Sreedharan Sajikumar
Summary: Clinical studies have shown that female brains are more predisposed to neurodegenerative diseases such as Alzheimer's disease, but the cellular and molecular mechanisms remain unknown. Our studies on mouse models suggest that AD impacts hippocampal long-term plasticity in a sex-specific manner, with female mice showing faster decay in memory and synaptic plasticity, accompanied by neuroinflammatory markers upregulation. This disparity may contribute to the faster progression of AD in females.
Article
Cell Biology
Wei Shi, Xinlei Sheng, Kerry M. Dorr, Josiah E. Hutton, James Emerson, Haley A. Davies, Tia D. Andrade, Lauren K. Wasson, Todd M. Greco, Yutaka Hashimoto, Joel D. Federspiel, Zachary L. Robbe, Xuqi Chen, Arthur P. Arnold, Ileana M. Cristea, Frank L. Conlon
Summary: This study demonstrates that sex disparities in cardiac function are not only controlled by sex hormones but also through a sex-chromosome mechanism, particularly involving X-linked gene dosage. The research also shows that cardiac sex disparities occur at the earliest stages of heart formation, before gonad development. Loss of A1BG is found to lead to cardiac defects in females but not in males.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Aloysius Y. T. Low, Nitsan Goldstein, Jessica R. Gaunt, Kuei-Pin Huang, Norliyana Zainolabidin, Alaric K. K. Yip, Jamie R. E. Carty, Ju Y. Choi, Alekso M. Miller, Helen S. T. Ho, Clara Lenherr, Nicholas Baltar, Eiman Azim, October M. Sessions, Toh Hean Ch'ng, Amanda S. Bruce, Laura E. Martin, Mark A. Halko, Roscoe O. Brady, Laura M. Holsen, Amber L. Alhadeff, Albert Chen, J. Nicholas Betley
Summary: Activity in anterior deep cerebellar nuclei reduces food consumption in mice without reducing metabolic rate, potentially identifying a therapeutic target for disorders involving excessive eating. The study defines a conserved satiation center that may represent a novel therapeutic target for the management of excessive eating.
Article
Biochemistry & Molecular Biology
Todd M. Greco, Christopher Secker, Eduardo Silva Ramos, Joel D. Federspiel, Jeh-Ping Liu, Alma M. Perez, Ismael Al-Ramahi, Jeffrey P. Cantle, Jeffrey B. Carroll, Juan Botas, Scott O. Zeitlin, Erich E. Wanker, Ileana M. Cristea
Summary: Huntington disease is a neurodegenerative disorder with multifactorial pathobiology. This study identifies perturbed protein-protein interactions involving the huntingtin protein, suggesting the influence of cellular factors on disease progression. The findings reveal that these interactions are affected by expanded polyglutamine and are associated with impaired synaptic communication and lysosomal acidification.
Editorial Material
Cell Biology
Sheeja Navakkode, Toh Hean Ch'ng, Sreedharan Sajikumar
Article
Neurosciences
Yee Song Chong, Lik-Wei Wong, Jessica Gaunt, Yan Jun Lee, Cai Shan Goh, Richard G. M. Morris, Toh Hean Ch'ng, Sreedharan Sajikumar
Summary: The amygdala plays a role in modulating hippocampal synaptic plasticity. The timing and activity level of amygdala stimulation are important factors for the induction and maintenance of long-term potentiation (LTP) in the ventral hippocampal area CA1. 100 Hz amygdala co-stimulation facilitates LTP induction and enhances persistence, while 200 Hz co-stimulation attenuates induction.
Article
Biology
Manutea C. Serrero, Virginie Girault, Sebastian Weigang, Todd M. Greco, Ana Ramos-Nascimento, Fenja Anderson, Antonio Piras, Ana Hickford Martinez, Jonny Hertzog, Anne Binz, Anja Pohlmann, Ute Prank, Jan Rehwinkel, Rudolf Bauerfeind, Ileana M. Cristea, Andreas Pichlmair, Georg Kochs, Beate Sodeik, Adam P. Geballe
Summary: This study identifies an interferon-inducible protein, MxB, that can sense herpesviral capsids and disassemble them, thereby restricting viral nuclear targeting and progeny capsid assembly, leading to enhanced innate immune responses.
Editorial Material
Neurosciences
Arnaldo Parra-Damas, Toh Hean Ch'ng, Bryen A. Jordan, Carlos A. Saura
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Microbiology
James P. Cwick, Jonathan E. Owen, Irina Kochetkova, Kyle S. Hain, Nick Van Horssen, Matthew P. Taylor
Summary: Most viruses utilize superinfection exclusion (SIE) to conserve resources and control population dynamics. This study provides new insights into how alphaherpesviruses limit sequential infection through the undercharacterized gD-independent SIE, which differs between PRV and HSV-1.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemical Research Methods
Michelle A. Kennedy, Todd M. Greco, Bokai Song, Ileana M. Cristea
Summary: Huntington's disease is a progressive neurological disorder caused by polyglutamine expansion of the huntingtin protein. Researchers have developed a web-based platform called HTT-OMNI to visualize and explore potential huntingtin protein interactors and their associated omics measurements.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Microbiology
Isabelle R. Taylor, Laura A. Murray-Nerger, Todd M. Greco, Dawei Liu, Ileana M. Cristea, Bonnie L. Bassler
Summary: This study reveals specific protein interactions involving PqsE and the role of RhlR in these interactions. These findings contribute to the understanding of the pathogenic mechanisms and quorum sensing process in Pseudomonas aeruginosa.