Article
Microbiology
Mingyu Han, Vincent Cantaloube-Ferrieu, Maorong Xie, Marie Armani-Tourret, Marie R. Woottum, Jean-Christophe Pages, Philippe R. Colin, Bernard Lagane, Serge R. Benichou
Summary: The study shows that HIV-1 can effectively infect macrophages through cell-to-cell transfer, even with virus isolates initially defined as non-macrophage tropic in cell-free infection. This intercellular viral transfer is facilitated by enhanced interactions between the HIV-1 envelope glycoproteins and cellular entry receptors.
Article
Multidisciplinary Sciences
Czernik Marta, Winiarczyk Dawid, Sampino Silvestre, Greda Pawel, Parillo Salvatore, Jacek Andrzej Modlinski, Loi Pasqualino
Summary: Studies have shown that in vitro culture conditions can affect mitochondrial dynamics and function, potentially leading to developmental abnormalities in embryos. This study investigated the effects of in vitro culture on mitochondrial function, distribution, and gene and protein expressions related to mitochondrial regulation. The results revealed that in vitro cultured embryos had fewer mitochondria, altered distribution and morphology, reduced mtDNA copy numbers, and lower expressions of proteins involved in mitochondrial fusion compared to in vivo embryos. These findings highlight the importance of optimizing culture conditions to maintain proper mitochondrial function and promote normal embryonic development.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Dairy & Animal Science
Giovanni Ianiro, Marina Monini, Maria Grazia Ammendolia, Luca De Sabato, Fabio Ostanello, Gabriele Vaccari, Ilaria Di Bartolo
Summary: This study successfully cultivated different subtypes of HEV-3 isolated from pig feces using a previously established protocol for HEV cultivation on human lung cells. Sequence analysis showed no insertion in the hypervariable region of the genomes and revealed a few mutations acquired during the growth on cells. The protocol allowed for quick production of a high amount of virus in serum-free medium and will be used for future experiments on virus infectivity.
Article
Gastroenterology & Hepatology
Cong Xu, Jieqing Fan, Danyang Liu, Aimaier Tuerdi, Juanjuan Chen, Yuning Wei, Yanfang Pan, Huaixin Dang, Xiong Wei, Ashraf Siddig Yousif, Jeysen Yogaratnam, Qiong Zhou, Henri Lichenstein, Tian Xu
Summary: The study demonstrates that DF-006 exhibits antiviral efficacy against HBV in mouse and PHH models without overt toxicity. In mice, DF-006 primarily targets the liver and activates innate immunity to mediate its anti-HBV effects.
Article
Multidisciplinary Sciences
Heng Zhou, Hanyan Li, Niket Gowravaram, Moqin Quan, Naila Kausar, Stephen N. Gomperts
Summary: The aggregates of Aβ associated with Alzheimer's disease perturb neural systems in a state-dependent manner, driving neuronal hyperactivity in exploratory behavior and slow wave sleep, while suppressing activity in quiet wakefulness and REM sleep.
SCIENTIFIC REPORTS
(2022)
Article
Virology
Nitin Kamble, Vishwanatha R. A. P. Reddy, Ben Jackson, Faisal R. Anjum, Chidiebere C. Ubachukwu, Ajit Patil, Shahriar Behboudi
Summary: Marek's disease virus (MDV) causes a deadly lymphoproliferative disease in chickens, resulting in huge economic losses in the poultry industry. It has been suggested that MDV suppresses the induction of type I interferons and thus escapes immune control. The genes CH25H and CYP27A1, which encode enzymes that have antiviral activities, may be useful in controlling MDV replication and spread.
Article
Virology
Kiira Kalke, Julius Orpana, Tuomas Lasanen, Olaya Esparta, Liisa M. Lund, Fanny Frejborg, Tytti Vuorinen, Henrik Paavilainen, Veijo Hukkanen
Summary: This study assessed the phenotype of 36 circulating HSV-1 strains from Finland to determine their potential as oncolytic HSV backbones. The results indicate that the phenotype of the circulating isolates, including their oncolytic potential, is closely related to the host cell type. The study emphasizes the importance of careful selection of the backbone virus in early vector design and highlights the potential of clinical isolates in oHSV development.
Article
Virology
Han Wan, Rebecca L. Adams, Brett D. Lindenbach, Anna Marie Pyle
Summary: Researchers have reported a complete secondary structure map of the HCV RNA genome, revealing its folding in the cellular context and demonstrating the importance of many structures in the virus life cycle. Furthermore, this work provides a resource for future studies aimed at identifying therapeutic targets and conducting further mechanistic studies on this important human pathogen.
JOURNAL OF VIROLOGY
(2022)
Article
Virology
V. Sozzi, L. McCoullough, H. Mason, M. Littlejohn, P. A. Revill
Summary: This article discusses the impact of splicing of hepatitis B virus (HBV) pre-genomic RNA on HBV replication and investigates the negative effects of a specific splice variant, Sp1, on genome-length HBV replication using a model that exceeds the genome length.
Article
Reproductive Biology
Anamaria-Cristina Herta, Nazli Akin, Katy Billooye, Laura Saucedo-Cuevas, Francesca Lolicato, Ingrid Segers, Ellen Anckaert, Johan Smitz
Summary: The study shows that oocytes from cultured mouse follicles can overcome complete denudation, restoring functional transzonal projections, with no significant differences in meiotic and developmental competence compared to those from intact cultured follicles. These findings provide good premises for developing successful strategies to rescue human oocyte competence in in vitro culture approaches.
BIOLOGY OF REPRODUCTION
(2021)
Article
Genetics & Heredity
Jian Zhao, Shi-Zhe Yu, Qiang Cai, Duo Ma, Long Jiang, Ling-Peng Yang, Zhi-Yong Yu
Summary: Key genes involved in cell cycle, DNA replication, and p53 signaling pathway were identified in the early stage of liver regeneration in mice after partial hepatectomy (PH). Ube2c was found to play a crucial role in regulating hepatocyte proliferation by modulating genes such as Ccna2, Cdkn1a, Chek1, and Mcm5.
FRONTIERS IN GENETICS
(2021)
Article
Pathology
Luiza B. Pires, Maria C. Peixoto-Rodrigues, Jessica F. Eloi, Cynthia M. Cascabulho, Helene S. Barbosa, Marcelo F. Santiago, Daniel Adesse
Summary: Congenital toxoplasmosis is a major cause of complications during pregnancy and after birth. This study found that infection of neural progenitor cells with Toxoplasma gondii can decrease cell proliferation, impair neurogenesis, and inhibit cell migration, which may contribute to cortical malformations in congenitally infected individuals.
AMERICAN JOURNAL OF PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rachel A. Caston, Paola Fortini, Kevin Chen, Jack Bauer, Eugenia Dogliotti, Y. Whitney Yin, Bruce Demple
Summary: After cellular differentiation, the expression of Fen1 and DNA2, which are involved in repairing mitochondrial DNA, decreases, while MGME1 and ExoG show minimal changes. Neuronal cells maintain mitochondrial DNA repair upon differentiation, relying on mitochondria-specific enzymes for BER.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Thomas Tu, Benno Zehnder, Jochen M. Wettengel, Henrik Zhang, Sally Coulter, Vikki Ho, Mark W. Douglas, Ulrike Protzer, Jacob George, Stephan Urban
Summary: Using in vitro models, the study found that after cell mitosis, there was a significant reduction in cccDNA levels, HBV-positive cell numbers, and cccDNA-dependent protein expression, supporting the efficacy of inducing cell mitosis to overcome HBV persistence.
Article
Immunology
Masataka Nakayama, Shigeru Kyuwa
Summary: This study aimed to determine the basic reproduction numbers (R-0) of three strains of MHV in mice. The results showed that the transmissibility of MHV is correlated with viral growth in the gastrointestinal tract of infected mice. This is the first report to address the basic reproduction numbers among pathogens in laboratory animals.
MICROBIOLOGY AND IMMUNOLOGY
(2022)
