Article
Fisheries
Hao Tang, Mingxue Sun, Wei Duan, Yanrong Ying, Yijing Liao, Tiaoyi Xiao, Yaoguo Li
Summary: In this study, the functional characterization of Nucleophosmin 1a (NPM1a) from grass carp (Ctenopharyngodon idellus) was deciphered. It was found that NPM1a played a negative regulatory role in the induction of Type I interferon reaction and GCRV replication. The study also identified the NES domain as crucial for nucleous-cytoplasm shuttle and GCRV replication. Overall, NPM1a is a potential target for GCRV infection treatment and resistance strain breeding in grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Oncology
Andrew B. Lassman, Patrick Y. Wen, Martin J. van den Bent, Scott R. Plotkin, Annemiek M. E. Walenkamp, Adam L. Green, Kai Li, Christopher J. Walker, Hua Chang, Sharon Tamir, Leah Henegar, Yao Shen, Mariano J. Alvarez, Andrea Califano, Yosef Landesman, Michael G. Kauffman, Sharon Shacham, Morten Mau-Sorensen
Summary: In patients with glioblastoma, single-agent Selinxor therapy achieved intratumoral penetration and demonstrated some efficacy with manageable side effects. Importantly, the dosage of Selinxor needs to be adjusted based on patient tolerability.
CLINICAL CANCER RESEARCH
(2022)
Article
Cell Biology
Yihu Xie, Shengyan Gao, Ke Zhang, Prasanna Bhat, Bradley P. Clarke, Kimberly Batten, Menghan Mei, Matthew Gazzara, Jerry W. Shay, Kristen W. Lynch, Alexia E. Angelos, Pate S. Hill, Austin L. Ivey, Beatriz M. A. Fontoura, Yi Ren
Summary: This study reveals the importance of the high-order SARNP/Tho1 complex interacting with DDX39B/RNA in mRNP assembly and export.
Article
Oncology
Jing Yang, Aiwen Jin, Jing Han, Xin Chen, Junnian Zheng, Yanping Zhang
Summary: MDMX plays an essential role in p53 degradation in vivo by recruiting UbcH5c to facilitate MDM2 E3 ligase function. The interaction between MDMX and UbcH5c is essential for MDMX to enable MDM2 E3 ligase activity for p53 degradation in the absence of MDMX. Together, these findings clarify the role of MDMX in the regulation of the critical tumor suppressor p53.
Article
Biochemistry & Molecular Biology
Guo-Qing Chen, Pei Li, Qing Yan, Yi-Hong Wu, Hao-Ran Wang, Shu-Fen Chao, Li-Juan Wu, Long Chen, Guo-Zhong Feng
Summary: Proteins with nuclear localization signals are transported into the nucleus through the importin-alpha/beta-mediated pathway. Importin-alpha proteins have been mainly studied in Drosophila, with little knowledge about them in Lepidoptera insects. This study identified four putative importin-alpha homologues in Sf9 cells, showing different nuclear localization patterns and specific interactions with AcMNPV DNA polymerase.
INSECT MOLECULAR BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Peter S. Choi, Andrei Thomas-Tikhonenko
Summary: The study found that a large number of RNA-binding proteins are mutated in cancer, leading to abnormal RNA processing activities, which is unusual in normal cells. These abnormal activities may create therapeutic vulnerabilities, which are being exploited in the clinic.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Youjin Na, Ashley Hall, Yanan Yu, Liang Hu, Kwangmin Choi, Jake A. Burgard, Sara Szabo, Gang Huang, Nancy Ratner, Jianqiang Wu
Summary: Neurofibromatosis type 1 (NF1) patients are prone to develop plexiform neurofibromas (PNFs), and all three endoplasmic reticulum (ER) stress response pathways are activated in PNFs. Inhibition of protein kinase RNA [PKR]-like ER kinase (PERK) can reduce the number of neurofibroma-spheres and tumor-like lesions in mouse models. Deletion of PERK in Schwann cells (SCs) and Schwann cell precursors (SCPs) leads to reduced tumor size, number, and increased survival in a PNF mouse model. Targeting proteostasis could be a potential therapy for PNFs.
Article
Multidisciplinary Sciences
Liuke Sun, Huihui Kong, Mengmeng Yu, Zhenyu Zhang, Haili Zhang, Lei Na, Yuxing Qu, Yuan Zhang, Hualan Chen, Xiaojun Wang
Summary: Species differences in ANP32A/B restrict avian influenza virus in mammalian cells. Adaptive mutations, such as PB2-E627K, are required for efficient replication of avian influenza viruses in mammals. We found that NS2 protein promotes avian vRNP assembly and enhances interactions with mammalian ANP32A/B, overcoming the restriction. Disrupting the conserved SUMO-interacting motif (SIM) in NS2 impairs virus replication and pathogenicity in mammals, indicating NS2 as a cofactor in the adaptation process of avian influenza virus to mammals.
Article
Oncology
Yali Wang, Weimin Zhang, Wenzhong Liu, Lijie Huang, Yan Wang, Dan Li, Guangchao Wang, Zitong Zhao, Xinming Chi, Yu Xue, Yongmei Song, Xuefeng Liu, Qimin Zhan
Summary: This study revealed a novel lncRNA-guided mechanism of lymph node metastasis in ESCC, in which VESTAR played a key role in lymphatic metastasis. This may provide a potential target for the treatment of ESCC lymphatic metastasis.
Article
Oncology
Erlene K. Seymour, Husain Yar Khan, Yiwei Li, Mahmoud Chaker, Irfana Muqbil, Amro Aboukameel, Radhakrishanan Ramchandren, Christiane Houde, Golbon Sterbis, Jay Yang, Divaya Bhutani, Silvana Pregja, Kathy Reichel, Amy Huddlestun, Colleen Neveux, Kelly Corona, Yosef Landesman, Jatin Shah, Michael Kauffman, Sharon Shacham, Ramzi M. Mohammad, Asfar S. Azmi, Jeffrey A. Zonder
Summary: The study demonstrated that SINE compounds enhance the activity of CHOP in non-Hodgkin lymphoma both in vitro and in vivo. Selinexor in combination with R-CHOP showed good tolerability and promising efficacy in patients with NHL.
CLINICAL CANCER RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Robin Dorau, Jun Chen, Jianming Liu, Peter Ruhdal Jensen, Christian Solem
Summary: Lactococcus lactis subsp. lactis, a model lactic acid bacterium, was subjected to adaptive laboratory evolution to improve thermotolerance, resulting in the isolation of the RD07 strain capable of efficient acidification at high temperatures. RD07 showed accelerated autolysis and potential to speed up cheese ripening, with transcriptomics analysis revealing the molecular mechanisms underlying these traits. Incorporating high thermotolerance L. lactis strains into dairy applications could simplify starter cultures, enhance cheese manufacturing efficiency, and introduce new variants of harder cheeses.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biology
Jasmin van den Heuvel, Caroline Ashiono, Ludovic C. Gillet, Kerstin Dorner, Emanuel Wyler, Ivo Zemp, Ulrike Kutay
Summary: In this study, it was found that the ribosomal protein eS30 is synthesized with the ubiquitin-like protein FUBI in human cells, but FUBI is cleaved off during ribosomal subunit maturation. Deubiquitinase USP36 was identified as an enzyme involved in FUBI-eS30 processing, demonstrating its functional importance in ribosomal subunit maturation and identifying a novel protease in the process.
Article
Plant Sciences
Mingzhen Zhang, Pan Gong, Linhao Ge, Zhaoyang Chang, Xiaofei Cheng, Xueping Zhou, Aiming Wang, Fangfang Li
Summary: The study reveals that XPO1 plays a crucial role in TuMV infection by interacting with NIb to facilitate its translocation to viral replication complexes, as well as mediating the nuclear export of sumoylated NIb and host factors tagged with SUMO3 to suppress plant immunity. Deficiencies of XPO1 in Arabidopsis and Nicotiana benthamiana plants inhibit TuMV replication and infection, highlighting the importance of XPO1 as a host factor in potyvirus infections.
Article
Oncology
Sanhita Mitra, Somsundar Veppil Muralidharan, Mirco Di Marco, Prasanna Kumar Juvvuna, Subazini Thankaswamy Kosalai, Silke Reischl, Daniel Jachimowicz, Santhilal Subhash, Ivan Raimondi, Leo Kurian, Maite Huarte, Per Kogner, Matthias Fischer, John Inge Johnsen, Tanmoy Mondal, Chandrasekhar Kanduri
Summary: The study reveals how a tumor suppressor called NBAT1 in neuroblastoma regulates p53 subcellular levels, affecting resistance to genotoxic drugs. Depletion of NBAT1 alters CRM1 function and contributes to the loss of p53-dependent nuclear gene expression, while inhibition of CRM1 rescues p53-dependent nuclear functions.
Article
Immunology
Laura Amato, Lucija Jurisic, Ilaria Puglia, Valeria Di Lollo, Valentina Curini, Giuseppe Torzi, Arturo Di Girolamo, Iolanda Mangone, Adamo Mancinelli, Nicola Decaro, Paolo Calistri, Francesca Di Giallonardo, Alessio Lorusso, Nicola D'Alterio
Summary: Various lineages of SARS-CoV-2 are circulating globally. In Abruzzo region, central Italy, diagnostic activities revealed a unique synonymous mutation in the B.1.177.75 lineage, leading to negative N gene tests in some strains. The majority of cases with negative N gene tests are genetically connected and may share a common origin, highlighting the importance of sharing genomic data for linking seemingly unrelated epidemiological clusters.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Multidisciplinary Sciences
Peter Reuther, Kristina Goepfert, Alexandra H. Dudek, Monika Heiner, Susanne Herold, Martin Schwemmle
SCIENTIFIC REPORTS
(2015)
Article
Biochemical Research Methods
Juergen Burger, Christin Rath, Johannes Woehrle, Philipp A. Meyer, Nessim Ben Ammar, Normann Kilb, Thomas Brandstetter, Florian Proell, Guenther Proll, Gerald Urban, Guenter Roth
Review
Biotechnology & Applied Microbiology
Normann Kilb, Juergen Burger, Guenter Roth
ENGINEERING IN LIFE SCIENCES
(2014)
Article
Biochemistry & Molecular Biology
Linda Brunotte, Joe Flies, Hardin Bolte, Peter Reuther, Frank Vreede, Martin Schwemmle
JOURNAL OF BIOLOGICAL CHEMISTRY
(2014)
Article
Virology
Peter Reuther, Benjamin Maenz, Linda Brunotte, Martin Schwemmle, Kerstin Wunderlich
JOURNAL OF VIROLOGY
(2011)
Article
Virology
Peter Reuther, Sebastian Giese, Veronika Goetz, David Riegger, Martin Schwemmle
JOURNAL OF VIROLOGY
(2014)
Article
Biochemical Research Methods
Geoffrey Chase, Kerstin Wunderlich, Peter Reuther, Martin Schwemmle
Article
Multidisciplinary Sciences
Benjamin Maenz, Linda Brunotte, Peter Reuther, Martin Schwemmle
NATURE COMMUNICATIONS
(2012)
Article
Biochemistry & Molecular Biology
Normann Kilb, Tobias Herz, Juergen Burger, Johannes Woehrle, Philipp A. Meyer, Guenter Roth
Article
Multidisciplinary Sciences
Federico Bertoglio, Doris Meier, Nora Langreder, Stephan Steinke, Ulfert Rand, Luca Simonelli, Philip Alexander Heine, Rico Ballmann, Kai-Thomas Schneider, Kristian Daniel Ralph Roth, Maximilian Ruschig, Peggy Riese, Kathrin Eschke, Yeonsu Kim, Dorina Schaeckermann, Mattia Pedotti, Philipp Kuhn, Susanne Zock-Emmenthal, Johannes Woehrle, Normann Kilb, Tobias Herz, Marlies Becker, Martina Grasshoff, Esther Veronika Wenzel, Giulio Russo, Andrea Kroeger, Linda Brunotte, Stephan Ludwig, Viola Fuehner, Stefan Daniel Kraemer, Stefan Duebel, Luca Varani, Gunter Roth, Luka Cicin-Sain, Maren Schubert, Michael Hust
Summary: COVID-19 is a severe acute respiratory disease caused by SARS-CoV-2, which utilizes human ACE2 enzyme as receptor for cell entry. Researchers have used phage display to select anti-SARS-CoV-2 spike antibodies from universal antibody gene libraries, showing potential inhibition of infection.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Marco Kunzli, Peter Reuther, Daniel D. Pinschewer, Carolyn G. King
Summary: The strength of TCR signal has different effects on the balance of Th1 and Tfh cells during acute versus persistent infection. Strong TCR signal favors Th1 generation during acute infection but hinders it during chronic infection. Weakly activated T cells show resistance to functional inactivation and display lower levels of markers associated with chronic T cell stimulation.
Article
Immunology
Peter Reuther, Katrin Martin, Mario Kreutzfeldt, Matias Ciancaglini, Florian Geier, Diego Calabrese, Doron Merkler, Daniel D. Pinschewer
Summary: Our study reveals that the systemic persistence of a prototypic noncytolytic RNA virus depends on continuous spread and reinfection. However, undefined cell-intrinsic mechanisms prevent viral persistence at the single-cell level, but lead to profound transcriptomic alterations in virus-cleared cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Helena Engel, Felix Guischard, Fabian Krause, Janina Nandy, Paulina Kaas, Nico Hoefflin, Maja Koehn, Normann Kilb, Karsten Voigt, Steffen Wolf, Tahira Aslan, Fabian Baezner, Salome Hahne, Carolin Ruckes, Joshua Weygant, Alisa Zinina, Emir Bora Akmeric, Enoch B. Antwi, Dennis Dombrovskij, Philipp Franke, Klara L. Lesch, Niklas Vesper, Daniel Weis, Nicole Gensch, Barbara Di Ventura, Mehmet Ali Oeztuerk
Summary: The use of D-peptides, composed of dextrorotatory enantiomers, offers improved stability and efficacy in peptide therapeutics. The web server finDr allows for the computational identification and optimization of D-peptide ligands to any protein structure, providing a tool for predicting optimal binders. This approach facilitates D-peptide discovery in biotechnology and biomedicine, presenting a cost-effective and user-friendly alternative to conventional methods.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)