Article
Biochemistry & Molecular Biology
Yun-Sang Tang, Wai-Kin So, Ka-Leung Andy Ng, Ka-Pun Chris Mok, Pang-Chui Shaw
Summary: This study identified host heterogeneous nuclear ribonucleoprotein C as an interacting partner of influenza A virus nucleoprotein and confirmed its negative regulatory role in viral growth. The findings not only enhance our understanding of the influenza virus transmission mechanism but also provide a new research direction for the development of anti-influenza drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Masahiro Nakano, Yukihiko Sugita, Noriyuki Kodera, Sho Miyamoto, Yukiko Muramoto, Matthias Wolf, Takeshi Noda
Summary: Nakano et al. utilize high-speed AFM and cryo-EM to investigate the structure of influenza A virus vRNPs during RNA synthesis, revealing two distinct types of vRNP structures associated with newly synthesized RNAs: an intact, helical structure and a deformed structure.
COMMUNICATIONS BIOLOGY
(2021)
Article
Microbiology
Julianna Han, Ketaki Ganti, Veeresh Kumar Sali, Carly Twigg, Yifeng Zhang, Senthamizharasi Manivasagam, Chieh-Yu Liang, Olivia A. Vogel, Iris Huang, Shanan N. Emmanuel, Jesse Plung, Lillianna Radoshevich, Jasmine T. Perez, Anice C. Lowen, Balaji Manicassamy
Summary: It is well documented that influenza A viruses selectively package 8 distinct viral ribonucleoprotein complexes (vRNPs) into each virion, but the exact role of host factors in genome assembly is not fully understood. This study shows that Rab11a+ vesicles play a crucial role in the congregation and assembly of vRNPs, enabling specific genome assembly and production of infectious virus particles. However, in the absence of Rab11a, viral RNA segments fail to congregate properly, resulting in defects in virus assembly and increased production of non-infectious particles.
Article
Biochemistry & Molecular Biology
Rachel J. Hagey, Menashe Elazar, Edward A. Pham, Siqi Tian, Lily Ben-Avi, Claire Bernardin-Souibgui, Matthew F. Yee, Fernando R. Moreira, Meirav Vilan Rabinovitch, Rita M. Meganck, Benjamin Fram, Aimee Beck, Scott A. Gibson, Grace Lam, Josephine Devera, Wipapat Kladwang, Khanh Nguyen, Anming Xiong, Steven Schaffert, Talia Avisar, Ping Liu, Arjun Rustagi, Carl J. Fichtenbaum, Phillip S. Pang, Purvesh Khatri, Chien-Te Tseng, Jeffery K. Taubenberger, Catherine A. Blish, Brett L. Hurst, Timothy P. Sheahan, Rhiju Das, Jeffrey S. Glenn
Summary: By targeting conserved RNA secondary structures, researchers have discovered a potential therapeutic approach that can effectively treat multiple viruses and has shown good efficacy and resistance in experiments. These findings are important for antiviral prophylaxis and post-exposure therapy.
Article
Multidisciplinary Sciences
Ian Seim, Ammon E. Posey, Wilton T. Snead, Benjamin M. Stormo, Daphne Klotsa, Rohit Pappu, Amy S. Gladfelter
Summary: This study reveals a mechanism that influences the composition of ribonucleoprotein bodies, in which specific sequence motifs and synergies with the intrinsically disordered region drive the formation of oligomers in the dilute phase, hindering its incorporation into the dense phase. This finding provides new insights for designing synthetic ribonucleoprotein bodies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Zihan Zhu, Ervin Fodor, Jeremy R. Keown
Summary: Influenza virus replication relies on the RNA-dependent RNA polymerase and viral ribonucleoprotein complex. Recent structural data have provided insights into the molecular mechanisms of influenza virus genome replication, particularly in terms of the encapsidation of nascent RNA, de novo assembly of ribonucleoprotein complexes, and the regulation of replication initiation through interactions with host and viral cues.
TRENDS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Franziska Guenl, Tim Krischuns, Julian A. Schreiber, Lea Henschel, Marius Wahrenburg, Hannes C. A. Drexler, Sebastian A. Leidel, Vlad Cojocaru, Guiscard Seebohm, Alexander Mellmann, Martin Schwemmle, Stephan Ludwig, Linda Brunotte
Summary: The replication of influenza A virus relies on ubiquitination of the viral polymerase derived from host cells. This study reveals that site-specific ubiquitination of PB1-K578 is acquired during infection and regulates the dimerization of polymerase and the binding of NP. Mass spectrometry analysis has identified 59 modified lysines across the three subunits of the viral polymerase, which affect mRNA transcription, vRNA replication, and the generation of recombinant viruses via non-proteolytic mechanisms.
NATURE COMMUNICATIONS
(2023)
Article
Plant Sciences
Mengjie Xiao, Tianbo Zhang, Fei Cao, Weihong Liang, Yinhua Yang, Tao Huang, Yun-Sang Tang, Bo Liu, Xin Zhao, Pang-Chui Shaw
Summary: This study discovered that tiliroside is the anti-influenza active component in Fu Rong Ye, which can inhibit the growth of four IAV strains in MDCK cells by suppressing viral ribonucleoprotein activity. Tiliroside also downregulates the expression of cytokines/chemokines in A549 cells and protects PR8-infected BALB/c mice from death, improving lung edema conditions. These findings suggest that tiliroside shows promise for influenza virus treatment and further drug development.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Public, Environmental & Occupational Health
Olga Syrkina, Ajinkya Inamdar, Sophie Wague, Celine Monfredo, Markku Nissila, Anne-Laure Chabanon, Laurence Serradell
Summary: This study conducted enhanced safety surveillance for the quadrivalent inactivated split-virion influenza vaccine (IIV4) in Finland during the 2020/21 season. The results showed no clinically significant changes in what is known or expected for IIV4, supporting the safety profile of this vaccine and maintaining public confidence in influenza vaccination.
Article
Multidisciplinary Sciences
Kara D. Wyatt, Demba Sarr, Kaori Sakamoto, Wendy T. Watford
Summary: Tpl2 plays a basal regulatory role in the antiviral response in lung epithelial cells during influenza A virus infection, but does not have a significant impact on host cytokine production or lung pathology.
Review
Biochemistry & Molecular Biology
Celia Jakob, Rithu Paul-Stansilaus, Martin Schwemmle, Roland Marquet, Hardin Bolte
Summary: The genome of influenza A virus consists of eight unique viral RNA segments, and reassortment events can create new IAVs. Recent research suggests that a viral genome packaging mechanism promotes reassortment by delivering the genome segments into the virus particle. However, there is a current shortage of functionally validated intersegmental RNA-RNA interactions in understanding this mechanism.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Microbiology
Nandita Kedia, Saptarshi Banerjee, Arindam Mondal
Summary: The study presents a novel cloning strategy and extensive standardization of the reporter RNP/polymerase activity assay for influenza B virus research. The assay system not only demonstrated the impact of NS1 and PKCD on viral RNA synthesis, but also showed promising results in evaluating antiviral drugs targeting viral RNA synthesis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Monika Vilimova, Maud Contrant, Ramy Randrianjafy, Philippe Dumas, Endrit Elbasani, Paivi M. Ojala, Sebastien Pfeffer, Aurelie Fender
Summary: miRNAs are small regulatory RNAs involved in almost all biological processes, and little is known about the impact of their co-expression from clusters. This study focused on the regulatory mechanism controlling the expression of ten clustered pre-miRNAs from KSHV, identifying the importance of pre-miR-K1 and -K3 in the cleavage efficiency. Mutational analysis showed that the presence of a canonical pre-miRNA at the location is crucial, and the cluster is cleaved in a sequential manner, providing insights for inhibiting the expression of the whole set of miRNAs.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Medicine, Research & Experimental
Takeshi Noda
Summary: The genome of influenza A virus consists of eight segmented RNAs, which are now believed to be selectively packaged through interactions mediated by segment-specific genome packaging signals. Studies suggest that these interactions are facilitated by their specific RNA characteristics.
COLD SPRING HARBOR PERSPECTIVES IN MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Sebastien Eymieux, Yves Rouille, Olivier Terrier, Karin Seron, Emmanuelle Blanchard, Manuel Rosa-Calatrava, Jean Dubuisson, Sandrine Belouzard, Philippe Roingeard
Summary: Studies on SARS-CoV-2 have shown that early infection events involve clusters of single-membrane vesicles and annulate lamellae, with host cell-derived organelles transforming into virus factories later on.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Virology
Ritsuko Koga, Yukihiko Sugita, Takeshi Noda, Yusuke Yanagi, Shinji Ohno
JOURNAL OF VIROLOGY
(2015)
Article
Immunology
Tadashi Maemura, Satoshi Fukuyama, Yukihiko Sugita, Tiago J. S. Lopes, Tomomi Nakao, Takeshi Noda, Yoshihiro Kawaoka
JOURNAL OF INFECTIOUS DISEASES
(2018)
Article
Biochemistry & Molecular Biology
Shinichi Machida, Yoshimasa Takizawa, Masakazu Ishimaru, Yukihiko Sugita, Satoshi Sekine, Jun-ichi Nakayama, Matthias Wolf, Hitoshi Kurumizaka
Article
Microbiology
Tokiko Watanabe, Eiryo Kawakami, Jason E. Shoemaker, Tiago J. S. Lopes, Yukiko Matsuoka, Yuriko Tomita, Hiroko Kozuka-Hata, Takeo Gorai, Tomoko Kuwahara, Eiji Takeda, Atsushi Nagata, Ryo Takano, Maki Kiso, Makoto Yamashita, Yuko Sakai-Tagawa, Hiroaki Katsura, Naoki Nonaka, Hiroko Fujii, Ken Fujii, Yukihiko Sugita, Takeshi Noda, Hideo Goto, Satoshi Fukuyama, Shinji Watanabe, Gabriele Neumann, Masaaki Oyama, Hiroaki Kitano, Yoshihiro Kawaoka
CELL HOST & MICROBE
(2014)
Article
Biotechnology & Applied Microbiology
Yukihiko Sugita, Takeshi Noda, Hiroshi Sagara, Yoshihiro Kawaoka
JOURNAL OF GENERAL VIROLOGY
(2011)
Article
Multidisciplinary Sciences
Tokiko Watanabe, Maki Kiso, Satoshi Fukuyama, Noriko Nakajima, Masaki Imai, Shinya Yamada, Shin Murakami, Seiya Yamayoshi, Kiyoko Iwatsuki-Horimoto, Yoshihiro Sakoda, Emi Takashita, Ryan McBride, Takeshi Noda, Masato Hatta, Hirotaka Imai, Dongming Zhao, Noriko Kishida, Masayuki Shirakura, Robert P. de Vries, Shintaro Shichinohe, Masatoshi Okamatsu, Tomokazu Tamura, Yuriko Tomita, Naomi Fujimoto, Kazue Goto, Hiroaki Katsura, Eiryo Kawakami, Izumi Ishikawa, Shinji Watanabe, Mutsumi Ito, Yuko Sakai-Tagawa, Yukihiko Sugita, Ryuta Uraki, Reina Yamaji, Amie J. Eisfeld, Gongxun Zhong, Shufang Fan, Jihui Ping, Eileen A. Maher, Anthony Hanson, Yuko Uchida, Takehiko Saito, Makoto Ozawa, Gabriele Neumann, Hiroshi Kida, Takato Odagiri, James C. Paulson, Hideki Hasegawa, Masato Tashiro, Yoshihiro Kawaoka
Article
Multidisciplinary Sciences
Takeshi Noda, Yukihiko Sugita, Kazuhiro Aoyama, Ai Hirase, Eiryo Kawakami, Atsuo Miyazawa, Hiroshi Sagara, Yoshihiro Kawaoka
NATURE COMMUNICATIONS
(2012)
Article
Multidisciplinary Sciences
Yukihiko Sugita, Hideyuki Matsunami, Yoshihiro Kawaoka, Takeshi Noda, Matthias Wolf
Article
Biology
Masahiro Nakano, Yukihiko Sugita, Noriyuki Kodera, Sho Miyamoto, Yukiko Muramoto, Matthias Wolf, Takeshi Noda
Summary: Nakano et al. utilize high-speed AFM and cryo-EM to investigate the structure of influenza A virus vRNPs during RNA synthesis, revealing two distinct types of vRNP structures associated with newly synthesized RNAs: an intact, helical structure and a deformed structure.
COMMUNICATIONS BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Yoko Fujita-Fujiharu, Yukihiko Sugita, Yuki Takamatsu, Kazuya Houri, Manabu Igarashi, Yukiko Muramoto, Masahiro Nakano, Yugo Tsunoda, Ichiro Taniguchi, Stephan Becker, Takeshi Noda
Summary: This study determines the structure of the Marburg virus nucleoprotein-RNA complex and provides mechanistic insight into the helical assembly of the nucleocapsid.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Ryoji Suno, Yukihiko Sugita, Kazushi Morimoto, Hiroko Takazaki, Hirokazu Tsujimoto, Mika Hirose, Chiyo Suno-Ikeda, Norimichi Nomura, Tomoya Hino, Asuka Inoue, Kenji Iwasaki, Takayuki Kato, So Iwata, Takuya Kobayashi
Summary: Prostaglandin receptors play important roles in inflammation, immune response, reproduction, and cancer. This study presents the structure of the human EP3-G(i) signaling complex using single-particle cryo-EM and compares it with other subtypes of prostaglandin receptors.
Article
Multidisciplinary Sciences
Shangfan Hu, Yoko Fujita-Fujiharu, Yukihiko Sugita, Lisa Wendt, Yukiko Muramoto, Masahiro Nakano, Thomas Hoenen, Takeshi Noda
Summary: In this study, the structure of the Lloviu virus nucleoprotein-RNA helical complex was determined using cryoelectron microscopy. The results showed that the N-terminal region of the nucleoprotein determines the helical arrangement, and identified amino acids involved in helical assembly and viral RNA synthesis. These findings advance our understanding of filovirus nucleocapsid formation and have implications for antiviral therapeutics development.
Article
Biochemistry & Molecular Biology
Si-Si Liu, Fei Jin, Yi-Shi Liu, Yoshiko Murakami, Yukihiko Sugita, Takayuki Kato, Xiao-Dong Gao, Taroh Kinoshita, Motoyuki Hattori, Morihisa Fujita
Summary: Glycosylphosphatidylinositol (GPI) anchor modification is a conserved posttranslational protein modification in eukaryotes, mediated by the GPI-transamidase (GPI-TA) complex. Functional residues of the five subunits of GPI-TA were analyzed by comparing conserved sequences among homologous proteins, and the purification method for studying its structure was optimized. Preliminary single particle images were obtained using purified GPI-TA, providing guidance for structural and functional analysis.