Article
Gastroenterology & Hepatology
Mousumi Khatun, Subhayan Sur, Robert Steele, Ranjit Ray, Ratna B. Ray
Summary: This study revealed that HCV exploits Linc-Pint in hepatocytes to enhance lipogenesis, shedding light on its role in virus replication and liver disease progression.
Article
Virology
Soroth Chey, Juliane Maria Palmer, Laura Doerr, Uwe Gerd Liebert
Summary: Reverse genetics is a powerful tool for analyzing viral genes, developing vaccines, and gene delivery vectors. A new reverse genetics platform has been established to increase the recovery efficiency of measles virus in human cells.
Article
Virology
Lisa Oestereich, Stephanie Wurr, Beate Becker-Ziaja, Sabrina Bockholt, Meike Pahlmann, Daniel Cadar, Beate M. Kuemmerer, Stephan Guenther, Romy Kerber
Summary: Highly pathogenic Arenaviruses, like the Lassa Virus (LASV), pose a serious public health threat in affected countries. Research and development of vaccines and therapeutics are urgently needed but hampered by the necessity to handle these pathogens under biosafety level 4 conditions. These containment restrictions make large-scale screens of antiviral compounds difficult. Therefore, the Mopeia virus (MOPV), closely related to LASV, is often used as an apathogenic surrogate virus.
Article
Biochemistry & Molecular Biology
Jia-Qi Li, Zhe-Rui Zhang, Hong-Qing Zhang, Ya-Nan Zhang, Xiang-Yue Zeng, Qiu-Yan Zhang, Cheng-Lin Deng, Xiao-Dan Li, Bo Zhang, Han-Qing Ye
Summary: The study demonstrated a safe and cost-effective method to prevent SARS-CoV-2 infection by expressing neutralizing antibodies in the lungs. Through in vitro and in vivo assays, it was shown that self-replicating mRNA could efficiently block SARS-CoV-2 infection with reduced viral titer and tissue damage in the lung with the help of lung target delivery using the VRP system.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Gastroenterology & Hepatology
Elodie Beaumont, Vincent Larochette, Laurence Preisser, Charline Miot, Pascale Pignon, Simon Blanchard, Bjorn-Thore Hansen, Jonathan Dauve, Caroline Poli, Minna M. Poranen, Patricia Lamourette, Marc Plaisance, Alain Morel, Helmut Fickenscher, Pascale Jeannin, Philippe Roingeard, Yves Delneste
Summary: IL-26 plays a crucial role in protecting against HCV infection in the liver of patients with chronic hepatitis C, exerting direct antiviral activity by inhibiting viral replication and interfering with RNA polymerase activity.
JOURNAL OF HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Reina Sasaki-Tanaka, Toshikatsu Shibata, Hiroaki Okamoto, Mitsuhiko Moriyama, Tatsuo Kanda
Summary: In this study, several novel therapeutic drugs were investigated through a drug repositioning approach, with favipiravir identified as a potential antiviral against hepatitis A virus (HAV) infection. The study examined the replication of HAV and introduced nucleotide mutations in the HAV genome using different drugs. Favipiravir showed inhibitory effects on HAV replication and introduced more nucleotide mutations compared to ribavirin. Further in vivo experiments are needed to confirm the efficacy of favipiravir in treating severe HAV infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Hye Won Kwak, Hyo-Jung Park, Seo-Yeon Jung, Eun Young Oh, Sang-In Park, Yeonhwa Kim, Hyeong-Jun Park, Sohyun Park, You-Jin Kim, Hae Li Ko, Jung-Ah Lee, Hyeran Won, Yun-Ho Hwang, Seo Yeon Kim, Se Eun Kim, Seoung Eun Bae, Minhyuk Yoon, Jae-Ouk Kim, Manki Song, Su Jeen Lee, Ki-Weon Seo, Kunse Lee, Dokeun Kim, Hun Kim, Sang-Myeong Lee, So-Hee Hong, Jae-Hwan Nam
Summary: Due to the rapid spread of SARS-CoV-2 and its variants, the development of effective and safe vaccines has become a priority. In this study, a recombinant MeV expressing the full-length SARS-CoV-2 spike protein (rMeV-S) was developed and its efficacy was tested in mouse and hamster models. The results showed that two-dose rMeV-S vaccination induced stronger immune responses compared to one-dose vaccination. Additionally, neutralizing antibodies induced by rMeV-S immunization effectively blocked the entry of different variants of SARS-CoV-2, and two-dose rMeV-S immunization provided complete protection against SARS-CoV-2 in the hamster model.
Article
Biochemistry & Molecular Biology
Shang Jui Tsai, Yiwei Ai, Chenxu Guo, Stephen J. Gould
Summary: Different antibiotic resistance genes have different thresholds for transgene expression, with BleoR gene having the highest threshold. Fusing antibiotic resistance proteins with degron tags can select for higher levels of recombinant protein expression. Furthermore, using certain antibiotic resistance genes as selection markers can increase the loading of proteins in exosomes. However, a binding peptide CP05 used in exosome decoration may bind to membranes non-specifically.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Dilara Z. Gatina, Ekaterina E. Garanina, Margarita N. Zhuravleva, Gulnaz E. Synbulatova, Adelya F. Mullakhmetova, Valeriya V. Solovyeva, Andrey P. Kiyasov, Catrin S. Rutland, Albert A. Rizvanov, Ilnur I. Salafutdinov
Summary: Coronary artery disease poses a significant healthcare challenge due to the high cost of treatment, increasing patient numbers, poor clinical outcomes, and limited effective therapies. Developing alternative therapeutic approaches for ischemic diseases, especially decompensated forms, is crucial. The use of multicistronic multigenic constructs encoding growth factors shows potential in promoting safe and effective systems for treating ischemic diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Timothy W. Thoner, Madeline M. Meloy, Jacob M. Long, Julia R. Diller, James C. Slaughter, Kristen M. Ogden
Summary: Reassortment, or genome segment exchange between viruses, is an important mechanism for generating novel virus genotypes and pandemic strains. This study investigates the factors influencing reassortment in reovirus, a segmented RNA virus, and finds that infection multiplicity and transcript abundance play key roles, whereas compartmentalization of replication and superinfection exclusion have minimal impact.
JOURNAL OF VIROLOGY
(2022)
Article
Gastroenterology & Hepatology
Yong He, Dechun Feng, Seonghwan Hwang, Bryan Mackowiak, Xiaolin Wang, Xiaogang Xiang, Robim M. Rodrigues, Yaojie Fu, Jing Ma, Tianyi Ren, Yeni Ait-Ahmed, Mingjiang Xu, Suthat Liangpunsakul, Bin Gao
Summary: IL-20 selectively inhibits hepatic IL-6 production rather than exerting IL-10-like broad anti-inflammatory properties. Unlike IL-22, IL-20 aggravates acute hepatitis and bacterial infection. Thus, anti-IL-20 therapy could be a promising option to control acute hepatitis and bacterial infection.
JOURNAL OF HEPATOLOGY
(2021)
Review
Medicine, General & Internal
Tarik Asselah, Mario Rizzetto
Summary: Hepatitis D virus (HDV), also known as hepatitis delta virus, is a defective, hepatotropic pathogenic agent. It requires the hepatitis B surface antigen (HBsAg) provided by hepatitis B virus (HBV) for its life cycle. HDV is mainly transmitted through contaminated syringes used by intravenous drug users. It is characterized by unique biological characteristics and severe medical effects. The global prevalence of HDV infection remains uncertain, and effective therapies are urgently needed.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Engineering, Environmental
Seongchan Kim, Ji-Seon Lee, Hyojin Lee
Summary: In this study, a dual-therapeutic platform using engineered graphene oxide (GO) as a multi-functional co-delivery carrier was developed. It successfully delivered two therapeutic reagents as a single vehicle, targeting multiple organelles in cells. The combination therapy showed enhanced efficacy in suppressing tumor growth and had a two-fold therapeutic efficiency compared to single-drug treatment.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Gastroenterology & Hepatology
Jun Zi, Yu-Huan Li, Xiao-Mei Wang, Hong-Qin Xu, Wen-Hui Liu, Jia-Yue Cui, Jun-Qi Niu, Xiu-Mei Chi
Summary: The screening practices for hepatitis D virus (HDV) are diverse and non-standardized worldwide, leading to uncertainty in the exact prevalence of HDV. This study aimed to estimate the prevalence of HDV and investigate the trends in viral marker quantity in patients with hepatitis D in Jilin Province, China. The results showed a correlation between HDV infection and HBsAg level, anti-HDAg level, and HBV DNA level.
WORLD JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Gastroenterology & Hepatology
Frauke Lange, Jonathan Garn, Holda A. Anagho, Florian W. R. Vondran, Thomas von Hahn, Thomas Pietschmann, Arnaud Carpentier
Summary: hPSC-derived HLCs become susceptible to HDV after acquiring the viral receptor NTCP. Infection leads to an innate immune response and upregulation of interferon-stimulated genes. Pre-treatment with IFN α2b reduces viral infection, while Myrcludex efficiently blocks infection and prevents immune activation.
LIVER INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Zhongliang Shen, Yanfeng Liu, Mengjun Luo, Wei Wang, Jing Liu, Wei Liu, Shaokun Pan, Youhua Xie
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2016)
Article
Microbiology
Wei Liu, Ruifeng Yang, Aimee S. Payne, Rachel M. Schowalter, Megan E. Spurgeon, Paul F. Lambert, Xiaowei Xu, Christopher B. Buck, Jianxin You
CELL HOST & MICROBE
(2016)
Article
Virology
Wei Liu, Margo MacDonald, Jianxin You
CURRENT OPINION IN VIROLOGY
(2016)
Article
Multidisciplinary Sciences
Ranran Wang, Xing-Jun Cao, Katarzyna Kulej, Wei Liu, Tongcui Ma, Margo MacDonald, Cheng-Ming Chiang, Benjamin A. Garcia, Jianxin You
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Multidisciplinary Sciences
Zhongliang Shen, Huijuan Yang, Sisi Yang, Wei Wang, Xiaoxian Cui, Xian Zhou, Wei Liu, Shaokun Pan, Yanfeng Liu, Junqi Zhang, Jiming Zhang, Youhua Xie, Jing Liu
NATURE COMMUNICATIONS
(2017)
Article
Oncology
Ranran Wang, Wei Liu, Christine M. Helfer, James E. Bradner, Jason L. Hornick, Susan M. Janicki, Christopher A. French, Jianxin You
Article
Biochemistry & Molecular Biology
W. Liu, P. Stein, X. Cheng, W. Yang, N-Y Shao, E. E. Morrisey, R. M. Schultz, J. You
CELL DEATH AND DIFFERENTIATION
(2014)
Article
Multidisciplinary Sciences
Wei Liu, Nathan A. Krump, Christopher B. Buck, Jianxin You
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Multidisciplinary Sciences
Wei Liu, Gloria B. Kim, Nathan A. Krump, Yuqi Zhou, James L. Riley, Jianxin You
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Virology
Wei Liu, Jianxin You
ANNUAL REVIEW OF VIROLOGY, VOL 7, 2020
(2020)
Article
Virology
Nathan A. Krump, Ranran Wang, Wei Liu, June F. Yang, Tongcui Ma, Jianxin You
Summary: MCPyV, which can cause Merkel cell carcinoma, infects most humans asymptomatically. Understanding MCPyV biology and its interaction with host immune response may help develop targeted therapies for MCC. MCPyV replication induces an innate immune response, involving cGAS-STING, NF-kappa B, and IFI16 pathways.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Wei Liu, Hanako M. Reyes, June F. Yang, Yize Li, Kathleen M. Stewart, Maria C. Basil, Susan M. Lin, Jeremy Katzen, Edward E. Morrisey, Susan R. Weiss, Jianxin You
Summary: The COVID-19 pandemic has posed a serious threat to global health, and research has found that activating the STING signaling pathway can effectively inhibit coronavirus infection, potentially serving as a promising target for future treatments against various strains of coronaviruses.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wei Liu, Mohamad-Gabriel Alameh, June F. F. Yang, Jonathan R. R. Xu, Paulo J. C. Lin, Ying K. K. Tam, Drew Weissman, Jianxin You
Summary: This study demonstrated that mRNA-lipid nanoparticle technology could efficiently deliver STING(R284S) into cancer cells to reactivate antitumor immunity and kill tumor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Virology
Ranran Wang, June F. Yang, Taylor E. Senay, Wei Liu, Jianxin You
Summary: Merkel cell carcinoma (MCC) is a lethal skin cancer that has increased in incidence in recent years. Merkel cell polyomavirus (MCPyV) is associated with a majority of MCC cases. In this study, the researchers found that MCPyV infection induces the expression of interferons (IFNs) and IFN-stimulated genes (ISGs) in human dermal fibroblasts (HDFs), but the IFN-mediated induction of ISGs is not crucial for viral control. Instead, type I IFN directly represses viral transcription. The researchers also found that growth factors induced by skin abrasion or UV irradiation can stimulate MCPyV gene expression and replication, supporting persistent infection.
JOURNAL OF VIROLOGY
(2023)
Article
Biology
Wei Liu, Nathan A. Krump, Meenhard Herlyn, Jianxin You