Article
Virology
Tingting Ma, Shuai Fu, Kun Wang, Yaqin Wang, Jianxiang Wu, Xueping Zhou
Summary: The study demonstrates that palmitoylation plays a crucial role in the plasma membrane localization and accumulation of group 1 remorin proteins in Nicotiana benthamiana. Among them, NbREM1.5 is able to significantly inhibit tobacco mosaic virus cell-to-cell movement by interacting with the virus's movement protein.
Review
Biochemistry & Molecular Biology
F. Murilo Zerbini, Elliot W. Kitajima
Summary: This article discusses the discovery of viruses as agents of diseases and their classification efforts throughout history. It highlights the creation of the International Committee on Nomenclature of Viruses in 1966, which led to the establishment of an official taxonomy for viruses based on evolutionary relationships.
Article
Biochemistry & Molecular Biology
Jianting Zhang, Ranjith Kumar Kankala, Jingyao Ma, Yihao Zhou, Shi-Bin Wang, Ai-Zheng Chen
Summary: Utilizing the hollow tobacco mosaic virus coat protein as a restrictive template, well-organized one-dimensional inorganic nanostructures were successfully fabricated with high yields, including gold nanoparticles and silver sulfide quantum dots. Different types of functional nanoparticles were encapsulated and assembled into ordered 1D nanochains through TMV-assisted self-assembly approach, showing potential for the fabrication of highly complex hybrid-metal nanostructures.
Article
Plant Sciences
Changyun Liu, Shaorui Tian, Xing Lv, Yundan Pu, Haoran Peng, Guangjin Fan, Xiaozhou Ma, Lisong Ma, Xianchao Sun
Summary: The study identified the important role of asparagine synthetase in plant immunity, demonstrating that it can enhance tobacco resistance to tobacco mosaic virus by improving the synthesis of asparagine. It was also found that external application of asparagine can inhibit virus infection by activating the salicylic acid signaling pathway.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Microbiology
Sruthy Maria Augustine, Stavros Tzigos, Rod Snowdon
Summary: Since the discovery of the tobacco mosaic virus in the 1890s, there has been an increasing awareness of the impact of viruses on the environment. Not only do viral infections cause disease, but some viruses can also have a beneficial effect on plants. This study shows that heat-killed tobacco mosaic virus can enhance abiotic stress tolerance in plants, which has not been reported before.
Article
Biochemistry & Molecular Biology
Zhi-Yong Yan, Xiao-Jie Xu, Le Fang, De-Jie Cheng, Yan-Ping Tian, Chao Geng, Xiang-Dong Li, Jari P. T. Valkonen
Summary: The study revealed that the two basic residues R-192 and K-225 in potyviral CP are crucial for viral RNA binding and cell-to-cell movement, with mutations in these residues affecting virus replication and movement between plant cells.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2021)
Article
Plant Sciences
Hongyan Han, Jialing Zou, Jingya Zhou, Mengyuan Zeng, Dongchao Zheng, Xuefeng Yuan, Dehui Xi
Summary: The small GTPase NtRHO1 plays a negative role in tobacco response to TMV infection by interacting with transcription factor NtWRKY50, resulting in reduced plant immunity.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Microbiology
Hao Hong, Chunli Wang, Ying Huang, Min Xu, Jiaoling Yan, Mingfeng Feng, Jia Li, Yajie Shi, Min Zhu, Danyu Shen, Peijun Wu, Richard Kormelink, Xiaorong Tao
Summary: This study found that antiviral RNAi mainly targets tospovirus mRNAs, while their genomic RNA is protected in RNPs against RISC-mediated cleavage. These findings suggest that the RNA targets of RISC differ between plant NSVs and ss(+)RNA plant viruses, indicating a potential counter-defense mechanism for NSVs to evade RISC-mediated degradation.
Article
Biochemistry & Molecular Biology
Zhe Cao, Xiaozhou Ma, Dashu Lv, Jing Wang, Yang Shen, Shiqi Peng, Shenggang Yang, Jin Huang, Xianchao Sun
Summary: In this study, a novel nanomaterial (ChNC@Zn) was prepared by using chitin nanocrystals loaded with Zn2+, which has the function of inducing disease resistance to plants and reducing virus activity. ChNC@Zn can improve the enzymes activities of peroxidase (POD) and catalase (CAT) in tobacco, and reduce the damage of reactive oxygen species (ROS) caused by TMV infection, thereby inducing resistance to TMV in tobacco. It can exhibit strong antiviral activity at low Zn2+ concentration and minimize the pollution of Zn2+ to the environment, which has high potential application value in the control of virus disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Juliane Schuphan, Ulrich Commandeur
Summary: Plant virus nanoparticles can be utilized as promising platforms for enzyme immobilization, but the interference of carrier on substrate binding led researchers to explore the suitability of utilizing tobacco mosaic virus (TMV) instead of potato virus X (PVX). Despite TMV allowing more enzyme molecules to be immobilized, the rigid scaffold and helical spacing of TMV significantly affected enzyme activity.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Engineering, Environmental
Muhammad Adeel, Tahir Farooq, Jason C. White, Yi Hao, Zifu He, Yukui Rui
Summary: The study demonstrates the protective role of carbon-based nanomaterials in inhibiting viral symptoms by hindering physical movement and viral replication. Plants treated with carbon nanomaterials showed normal phenotype and reduced viral infection symptoms, with photosynthesis comparable to healthy controls. Upregulation of defense-related phytohormones and transcription of genes responsible for phytohormone biosynthesis were also observed in treated plants.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Narjes Sheibani, Seyed Shahriar Arab, Mohammad Kamalvand
Summary: The coat protein of Tobacco Mosaic Virus plays a crucial role in protecting the virus RNA. The interaction between the virus coat protein and host plant proteins leads to infection and mosaic patterns on crops, affecting plant growth and function. By studying mutants of the coat protein through molecular dynamics simulation, this study confirms their role in coat protein-mediated resistance against TMV infection.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Pharmacology & Pharmacy
Elahehnaz Parhizkar, Parivash Rafieipour, Alireza Sepasian, Effat Alemzadeh, Ali Dehshahri, Fatemeh Ahmadi
Summary: Gemcitabine, a nucleoside analogue drug, has limitations due to rapid body clearance, but chemical conjugation with Tobacco Mosaic Virus shows potential for drug delivery with minimal toxicity. In vitro studies suggest improved cytotoxicity and drug release from the conjugate, indicating potential for better pharmacokinetics and reduced toxicity in vivo.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Sachin N. Shah, Jonathan G. Heddle, David J. Evans, George P. Lomonossoff
Summary: We demonstrate a simple and low-energy method to template the synthesis of nanowires and particles consisting of gold, platinum, and palladium using tomato mosaic virus particles. Selective nanowire growth and deposition of alloy nanoparticles on the external surface of the rods were achieved by controlling the reaction conditions.
Article
Plant Sciences
Bernd Zechmann, Maria Mueller, Stefan Moestl, Gunther Zellnig
Summary: TMV and ZYMV infections in tobacco and pumpkin plants respectively lead to significant ultrastructural changes, including accumulation of virus inclusion bodies, alterations in mitochondria, peroxisomes, and chloroplasts, as well as changes in starch and plastoglobules volumes in chloroplasts. The comprehensive data collected in this study provide insights into the 3D changes induced by these viruses in plants.
Article
Microbiology
Manuel Bano-Polo, Luis Martinez-Gil, Manuel M. Sanchez del Pino, Alberto Massoli, Ismael Mingarro, Ruben Leon, Maria Jesus Garcia-Murria
Summary: The study showed that CPC can cause the rupture of SARS-CoV-2 VLP membranes, suggesting that including it in mouthwashes could be a prophylactic strategy to prevent the spread of the virus.
JOURNAL OF ORAL MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Gerard Duart, John Lamb, Juan Ortiz-Mateu, Arne Elofsson, Ismael Mingarro
Summary: Salt bridges play a crucial role in protein stabilization, especially in membrane proteins. The study found that potentially salt-bridge forming pairs are statistically over-represented in transmembrane helices. Experimental results showed that salt bridges contribute approximately 0.5 kcal/mol to the membrane insertion process during protein biogenesis.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Virology
David Villar-Alvarez, Vicente Pallas, Santiago F. Elena, Jesus A. Sanchez-Navarro
Summary: The results of this study challenge the traditional view on the role of virus particles in the systemic transport of plant viruses. It was shown that two different movement proteins can transport the AMV genome systemically without the need for virus particles. Additionally, it was found that the BMV MP is capable of short- and long-distance transport without interacting with the CP.
JOURNAL OF VIROLOGY
(2022)
Editorial Material
Virology
Vicente Pallas, Francesco Di Serio, Nobuhiro Suzuki
Article
Virology
Gustavo Gomez, Joan Marquez-Molins, German Martinez, Vicente Pallas
Summary: Viroid infection induces alterations in host DNA methylation, potentially influencing the plant epigenome.
Article
Virology
Joan Marquez-Molins, Vasti Thamara Juarez-Gonzalez, Gustavo Gomez, Vicente Pallas, German Martinez
Summary: This study uses a computational approach to analyze RNA modifications in plant viral and subviral RNAs and finds that they may contain a variety of previously unreported modifications. This opens up a new perspective in studying plant-pathogen interactions.
Article
Multidisciplinary Sciences
Gerard Duart, Assaf Elazar, Jonathan Y. Weinstein, Laura Gadea-Salom, Juan Ortiz-Mateu, Sarel J. Fleishman, Ismael Mingarro, Luis Martinez-Gil
Summary: Several methods have been developed to explore protein-protein interactions, but there is a need for more research on targeting transmembrane domains (TMDs). This study developed a computational approach to design sequences that can modulate protein-protein interactions in the membrane, and successfully applied it to BclxL. The findings enhance our understanding of protein-protein interactions in membranes and may lead to the development of inhibitors targeting TMD interactions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Virology
Ma Jesus Garcia-Murria, Laura Gadea-Salom, Sandra Moreno, Marina Rius-Salvador, Oscar Zaragoza, Alejandro Brun, Ismael Mingarro, Luis Martinez-Gil
Summary: In this study, a Bimolecular Multicellular Complementation (BiMuC) assay was developed to safely and efficiently monitor SARS-CoV-2 S-mediated membrane fusion without the need for microscopy-based equipment. Using BiMuC, a library of approved drugs was screened and compounds that enhance S protein-mediated cell-cell membrane fusion were identified, such as ethynylestradiol which promotes the growth of SARS-CoV-2 and Influenza A virus in vitro. These findings demonstrate the potential of BiMuC for identifying small molecules that modulate the life cycle of enveloped viruses, including SARS-CoV-2.
Article
Biochemistry & Molecular Biology
Laura Agullo, Isidro Aguado, Javier Muriel, Cesar Margarit, Alba Gomez, Monica Escorial, Astrid Sanchez, Alicia Fernandez, Ana M. Peiro
Summary: Variability in analgesic response due to genetic polymorphisms can be reduced through genotype-guided opioid prescribing. This study found that genotype-guided treatment led to improved pain relief, increased quality of life, and reduced adverse events and opioid dose in chronic pain patients. The findings suggest that implementing genotype-guided treatment could have positive clinical and economic outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Maria Saiz-Bonilla, Andrea Martin-Merchan, Vicente Pallas, Jose Antonio Navarro
Summary: This study demonstrates for the first time that an exogenous dual-targeted protein utilizes the general import systems to enter mitochondria and chloroplasts, and identifies the important role of plant receptors in this process. Additionally, the study reveals the interconnection between chloroplast protein translocation impairment, retrograde communication, and viral infection.
JOURNAL OF VIROLOGY
(2023)
Article
Plant Sciences
Joan Marquez-Molins, Pascual Villalba-Bermell, Julia Corell-Sierra, Vicente Pallas, Gustavo Gomez
Summary: This study provides an integrative analysis of the genome-wide alterations in cucumber plants infected with hop stunt viroid (HSVd). The results show that HSVd causes a reconfiguration of the cucumber regulatory pathways, predominantly affecting specific regulatory layers at different infection phases. The initial response involves differential exon usage, followed by transcriptional downregulation modulated by epigenetic changes. The alterations in endogenous small RNAs are limited and mainly occur at the late stage. The significant host alterations are primarily related to the downregulation of plant defense mechanisms and the systemic spreading of defense signals.
PLANT CELL AND ENVIRONMENT
(2023)
Article
Chemistry, Medicinal
Laura de Miguel, Pura Ballester, Cecilia Egoavil, Maria Luisa Sanchez-Ocana, Ana Maria Garcia-Munoz, Begona Cerda, Pilar Zafrilla, Enrique Ramos, Ana M. Peiro
Summary: This study aimed to investigate the impact of pharmacogenetic markers on the prevention of adverse events (AEs) in individuals with autism spectrum disorder (ASD) and intellectual disability (ID). The results showed a significant correlation between the DOP2 genotype and neurological AEs, suggesting that studying this gene could contribute to the safety of prescriptions for this population.
Article
Virology
Mikhail Oliveira Leastro, Elliot Watanabe Kitajima, Vicente Pallas, Jesus Angel Sanchez- Navarro
Summary: This study reports the construction and validation of an infectious cDNA clone of Citrus leprosis virus C (CiLV-C) based on an agroinfection system. Agro-injected Nicotiana benthamiana plants showed localized lesions similar to the natural infection process. The virus recovered from the infected plant tissue could be mechanically transmitted between plants. The CiLV-C reverse genetic system provides a powerful molecular tool for unraveling the peculiarities of the citrus leprosis pathosystem.