Editorial Material
Multidisciplinary Sciences
Mireille Farjo, Christopher B. Brooke
Summary: Influenza viruses infecting the same host can hinder each other's replication, leading to spatial structuring of infected cells in tissue and impacting viral evolution.
Review
Microbiology
Alvin X. Han, Simon P. J. de Jong, Colin A. Russell
Summary: This review summarizes the interplay between immunity evolution from previous infections or vaccination and the evolution of seasonal influenza viruses, including functional constraints, within-host evolutionary processes, and options for influenza virus control. It is found that seasonal influenza viruses continually evolve to escape host immunity, driven by viral constraints and host immune responses. However, it remains unclear how these advances can effectively reduce the impact of seasonal influenza on human health.
NATURE REVIEWS MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pierre Barrat-Charlaix, John Huddleston, Trevor Bedford, Richard A. Neher
Summary: Current frequency is the strongest predictor of eventual fixation, as expected in neutral evolution, while other properties have little predictive power. Parallel evolution is moderately predictive of fixation, and although LBI has limited capacity for predicting frequency dynamics, it can successfully select strains representative of future populations.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Immunology
Pauline M. van Diemen, Alexander M. P. Byrne, Andrew M. Ramsay, Samantha Watson, Alejandro Nunez, Ana v Moreno, Chiara Chiapponi, Emanuela Foni, Ian H. Brown, Sharon M. Brookes, Helen E. Everett
Summary: We investigated the infection dynamics of 2 influenza A(H1N1) virus isolates from the swine 1A.3.3.2 (pandemic 2009) and 1C (Eurasian, avian-like) lineages. The 1C-lineage virus, A/Pavia/65/2016, although phylogenetically related to swine-origin viruses, was isolated from a human clinical case. This strain infected ferrets, a human influenza model species, and could be transmitted by direct contact and, less efficiently, by airborne exposure. Our results reaffirm the importance of continuous influenza A virus surveillance in pigs and identification of candidate human vaccine viruses.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Immunology
Nina Urke Ertesvag, Rebecca Jane Cox, Sarah Larteley Lartey, Kristin G- Mohn, Karl Albert Brokstad, Mai-Chi Trieu
Summary: This study investigated the breadth and durability of influenza A/H3N2-specific antibodies in children and adults. It found that childhood infection increased adult antibody responses, and repeatedly vaccinated adults had broader and more durable antibodies.
Article
Multidisciplinary Sciences
Tingting Li, Junyu Chen, Qingbing Zheng, Wenhui Xue, Limin Zhang, Rui Rong, Sibo Zhang, Qian Wang, Minqing Hong, Yuyun Zhang, Lingyan Cui, Maozhou He, Zhen Lu, Zhenyong Zhang, Xin Chi, Jinjin Li, Yang Huang, Hong Wang, Jixian Tang, Dong Ying, Lizhi Zhou, Yingbin Wang, Hai Yu, Jun Zhang, Ying Gu, Yixin Chen, Shaowei Li, Ningshao Xia
Summary: Influenza A viruses are a significant global threat, and this study has identified a chimeric monoclonal antibody, C12H5, that offers broad neutralization against H1N1 and H5N1 viruses. The antibody targets a specific epitope on the surface glycoprotein of the virus, leading to control of virus entry and egress. This discovery could have implications for the development of antiviral drugs and broad-protection vaccines against influenza.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Heather M. Froggatt, Alfred T. Harding, Ryan R. Chaparian, Nicholas S. Heaton
Summary: The study identified ETS variant transcription factor 7 (ETV7) as a negative regulator of the type I IFN response, specifically targeting a subset of antiviral ISGs important for IFN-mediated control of influenza viruses. This research assigns a function for ETV7 as an IFN response regulator and suggests ETV7 as a potential therapeutic target to enhance innate antiviral responses and IFN-based antiviral therapies.
Review
Immunology
Furong Qing, Zhiping Liu
Summary: Interferon regulatory factor 7 (IRF7) is a crucial member of the IRFs family, located downstream of the pattern recognition receptors (PRRs)-mediated signaling pathway, and plays a vital role in the production of type I interferon (IFN-I). Its activation can inhibit various viral and bacterial infections, suppress the growth and metastasis of certain cancers, but it may also impact the tumor microenvironment and promote the development of other cancers.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Nungruthai Suntronwong, Preeyaporn Vichaiwattana, Lakkhana Wongsrisang, Sirapa Klinfueng, Sumeth Korkong, Thanunrat Thongmee, Nasamon Wanlapakorn, Yong Poovorawan
Summary: Evaluation of seroprevalence in older adults without a vaccination history revealed low levels of pre-existing antibodies to circulating influenza strains, with no age-dependent differences observed. Higher seropositive rates were found for influenza A viruses compared to influenza B viruses. Only a small percentage of individuals possessed broadly protective antibodies against both seasonal influenza A and B virus. This study will provide valuable information for healthcare planning and vaccination program control measures.
Article
Multidisciplinary Sciences
Nungruthai Suntronwong, Preeyaporn Vichaiwattana, Lakkhana Wongsrisang, Sirapa Klinfueng, Sumeth Korkong, Thanunrat Thongmee, Nasamon Wanlapakorn, Yong Poovorawan
Summary: This study found low levels of pre-existing antibodies to seasonal influenza virus subtypes in older adults and age-independent antibody levels. Moderate seropositive rates were observed for some influenza A viruses, while comparatively lower rates were found for influenza B viruses. Only a small percentage of individuals had broadly protective antibodies against both seasonal influenza A and B viruses.
Article
Biology
Pia Ryt-Hansen, Jesper Schak Krog, Solvej Ostergaard Breum, Charlotte Kristiane Hjulsager, Anders Gorm Pedersen, Ramona Trebbien, Lars Erik Larsen
Summary: This paper presents the results of swIAV surveillance in Danish swine from 2011 to 2018, revealing multiple different circulating genotypes of the virus, including novel reassortants with human seasonal IAV gene segments. The phylogenetic analysis showed genetic drift in antigenic sites of the hemagglutinin protein and identified a swine divergent cluster among the viruses studied.
Article
Biology
Jennifer E. Jones, Valerie Le Sage, Gabriella H. Padovani, Michael Calderon, Erik S. Wright, Seema S. Lakdawala
Summary: Influenza viruses evolve rapidly during infection through mutations or reassortment, potentially leading to strains with increased resistance or infectivity. Understanding how genome segments interact during reassortment can help predict future events and emergence of new pandemic strains. Research in this area could provide valuable insights for risk assessment and pandemic preparedness.
Article
Virology
Aitor Nogales, Michael Schotsaert, Raveen Rathnasinghe, Marta L. DeDiego, Adolfo Garcia-Sastre, Luis Martinez-Sobrido
Summary: The influenza A virus (IAV) can infect various mammalian and avian species, and studies have developed replication-competent IAV expressing traceable reporter genes to better understand its biology and pathogenesis. These novel approaches provide valuable tools for developing new therapeutic strategies against IAV infections.
Article
Immunology
Mariia V. Sergeeva, Ekaterina A. Romanovskaya-Romanko, Vera Z. Krivitskaya, Polina A. Kudar, Nadezhda N. Petkova, Kira S. Kudria, Dmitry A. Lioznov, Marina A. Stukova, Yulia A. Desheva
Summary: Neuraminidase-based immunity has the potential to mitigate the impact of novel antigenic variants of influenza viruses. The dynamics of anti-NA antibody response varies depending on the virus subtype, and the persistence of antibodies is different from that of anti-HA antibodies. The level of NA antibodies after vaccination correlates directly with the preexisting titers.
Article
Chemistry, Medicinal
Kris White, Matthew Esparza, Jue Liang, Prasanna Bhat, Jacinth Naidoo, Briana L. McGovern, Michael A. P. Williams, Busola R. Alabi, Jerry Shay, Hanspeter Niederstrasser, Bruce Posner, Adolfo Garcia-Sastre, Joseph Ready, Beatriz M. A. Fontoura
Summary: An inhibitor of the HA protein has been identified in this study, which prevents entry and replication of influenza viruses, showing potent antiviral activity against various subtypes. Important moieties for antiviral activity have been identified through synthesis of derivatives of this compound, suggesting its potential as a lead for drug development to enhance pan-subtype efficacy in combination with other influenza A virus antivirals.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Immunology
Katelyn Burleigh, Joanna H. Maltbaek, Stephanie Cambier, Richard Green, Michael Gale, Richard C. James, Daniel B. Stetson
SCIENCE IMMUNOLOGY
(2020)
Article
Immunology
Hayden N. Brochu, Elizabeth Tseng, Elise Smith, Matthew J. Thomas, Aiden M. Jones, Kayleigh R. Diveley, Lynn Law, Scott G. Hansen, Louis J. Picker, Michael Gale, Xinxia Peng
JOURNAL OF IMMUNOLOGY
(2020)
Article
Microbiology
Elizabeth J. Fay, Stephanie L. Aron, Marissa G. Macchietto, Matthew W. Markman, Katharina Esser-Nobis, Michael Gale, Steven Shen, Ryan A. Langlois
Article
Multidisciplinary Sciences
Sooyoung Lee, Ashish Goyal, Alan S. Perelson, Yuji Ishida, Takeshi Saito, Michael Gale
Summary: Activation of the RIG-I pathway and IRF3 can induce innate immune actions towards the elimination of cccDNA, showing therapeutic benefit in suppressing HBV infection.
Article
Immunology
Joseph M. Leal, Jessica Y. Huang, Karan Kohli, Caleb Stoltzfus, Miranda R. Lyons-Cohen, Brandy E. Olin, Michael Gale, Michael Y. Gerner
Summary: During type I inflammation, antigen-presenting dendritic cells in lymph nodes reposition to elicit T cell priming, while inflammatory monocytes infiltrate and cooperate with dendritic cells to optimize T cell effector differentiation.
SCIENCE IMMUNOLOGY
(2021)
Article
Microbiology
Matthew A. Burchill, Matthew P. Salomon, Lucy Golden-Mason, Amanda Wieland, Ana C. Maretti-Mira, Michael Gale, Hugo R. Rosen
Summary: Chronic HCV infection leads to dysregulation of innate immune responses and impaired T cell function. DAA therapy can induce significant changes in gene expression in T cells, offering a valuable data source to investigate the effects of DAA treatment on T cell populations.
Article
Immunology
Evan S. Walsh, Tammy S. Tollison, Hayden N. Brochu, Brian Shaw, Kayleigh R. Diveley, Hsuan Chou, Lynn Law, Allan D. Kirk, Michael Gale, Xinxia Peng
Summary: Recent advancements in microfluidics and high-throughput sequencing technologies have made it possible to analyze the immune repertoire of rhesus macaques at the single-cell level. Custom primers and a commercial solution were used to sequence the Ig and TCR repertoires, allowing for the recovery of every antibody isotype and TCR chain. The study also measured clonal expansion in T cells and paired the immune repertoire with gene expression profiles.
JOURNAL OF IMMUNOLOGY
(2022)
Editorial Material
Immunology
Ourania Andrisani, Qian Liu, Patricia Kehn, Wolfgang W. Leitner, Kyung Moon, Nancy Vazquez-Maldonado, Ian Fingerman, Michael Gale
Summary: The workshop aimed to review current advances and identify knowledge gaps in RNA helicases, leveraging them as molecular targets with translational potential.
Article
Immunology
Michael A. Davis, Kathleen Voss, J. Bryan Turnbull, Andrew T. Gustin, Megan Knoll, Antonio Muruato, Tien-Ying Hsiang, Kenneth H. Dinnon, Sarah R. Leist, Katie Nickel, Ralph S. Baric, Warren Ladiges, Shreeram Akilesh, Kelly D. Smith, Michael Gale
Summary: We conducted a comprehensive analysis of SARS-CoV-2 infection and recovery in mice and found that it closely resembles the acute human disease. Disease severity and infection kinetics were influenced by age and sex, with older mice and males exhibiting higher mortality rates and slower viral clearance. The pathological features observed in mice, including virus positivity in bronchial epithelial cells and lung damage, parallel those seen in humans. Our findings suggest that C57BL/6 mice can serve as a useful model for studying SARS-CoV-2 and other emerging coronaviruses.
Article
Biology
Hayden Brochu, Ruihan Wang, Tammy Tollison, Chul-Woo Pyo, Alexander Thomas, Elizabeth Tseng, Lynn Law, Louis J. Picker, Michael Gale, Daniel E. Geraghty, Xinxia Peng
Summary: RhCMV-based vaccination against SIV in rhesus macaques can effectively control SIV infection in approximately 55% of vaccinated monkeys by eliciting MHC-E-restricted CD8+ T cells. Analysis of the Mamu-E genomic sequences in rhesus macaques reveals gene duplication and different allele groups that are associated with the lack of vaccine protection.
COMMUNICATIONS BIOLOGY
(2022)
Article
Virology
Amy Y. Lu, Andrew Gustin, Daniel Newhouse, Michael Gale
Summary: This study compared the virologic properties and innate immune responses of African and Asian lineage Zika virus strains. The results showed that Asian lineage virus accumulates viral proteins earlier, replicates to higher levels, and strongly blocks interferon signaling during acute infection. The early accumulation of viral proteins promotes viral spread by antagonizing the host's immune response.
JOURNAL OF VIROLOGY
(2023)
Review
Virology
Autumn LaPointe, Michael Gale Jr, Alison M. Kell
Summary: Orthohantaviruses are rodent-borne, negative-sense RNA viruses that cause severe vascular disease in humans. These viruses have evolved mechanisms to avoid or counteract host innate immune responses, resulting in lifelong asymptomatic infections in the rodent reservoir. However, in other hosts, the ability to subdue the innate immune response may be less efficient or absent, leading to disease and viral clearance.
Article
Virology
Linhui Hao, Tien-Ying Hsiang, Ronit R. Dalmat, Renee Ireton, Jennifer F. Morton, Caleb Stokes, Jason Netland, Malika Hale, Chris Thouvenel, Anna Wald, Nicholas M. Franko, Kristen Huden, Helen Y. Chu, Alex Sigal, Alex L. Greninger, Sasha Tilles, Lynn K. Barrett, Wesley C. Van Voorhis, Jennifer Munt, Trevor Scobey, Ralph S. Baric, David J. Rawlings, Marion Pepper, Paul K. Drain, Michael Gale
Summary: New variants of SARS-CoV-2 continue to emerge and evade immunity. Convalescence from the ancestral virus provides limited protection against variants. Vaccination enhances immunity against viral variants, but the protection is weaker against Omicron BA.1, and a three-dose vaccine regimen provides significantly better protection against it compared to a two-dose.
Article
Microbiology
Yuan-Po Tu, Christopher Green, Linhui L. Hao, Alexander F. Greninger, Jennifer A. Morton, Heather Sights, Michael K. Gale Jr, Paul Drain
Summary: In this cross-sectional diagnostic study of 626 adults, the sensitivity of the antigen test to identify replication-competent SARS-CoV-2 was 63.6% in asymptomatic and 91.0% in symptomatic participants. Viral culture titers were the highest at the onset of symptoms and rapidly declined by 7 days after symptom onset.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Amanda Laine, Xiaoxing Wang, Kathryn Ni, Sarah E. B. Smith, Rayan Najjar, Leanne S. S. Whitmore, Michael M. Yacoub, Alison Bays, Michael Gale, Tomas Mustelin
Summary: Many patients with autoimmune diseases have autoantibodies against proteins encoded by genomic retroelements, suggesting a lack of immune tolerance. In this study, the researchers found that transmembrane envelope (Env) protein encoded by HERV-K102 is increased in rheumatoid arthritis (RA) patients, while HERV-K108 expression is higher in other immune cells. Patient autoantibodies recognized endogenously expressed Env in breast cancer cells and RA neutrophils. The findings suggest that HERV-K102 is the main contributor to cell surface Env on neutrophils in RA.