Article
Virology
Qiuhan Zhang, Siliang Li, Ping Lei, Zixian Li, Feifei Chen, Qi Chen, Yulu Wang, Jiami Gong, Qi Tang, Xinjin Liu, Ke Lan, Shuwen Wu
Summary: Our research reveals the crucial role of host factor ANXA2 in facilitating the formation of the EV71 RNA replication complex. ANXA2 interacts with 3D polymerase to promote EV71 replication and also interacts with PI4KB. Overexpression of ANXA2 stimulates PI4P formation, while knockout of ANXA2 leads to decreased levels of PI4P.
Article
Virology
Meixian Fu, Jinjin Bai, Shuang Gao, Zhangmei Chang, Xin Zhou, Jian-Er Long
Summary: This study describes a method for rapid rescue of infectious EV71 by using cells expressing T7 polymerase, which involves cloning the full-length EV71 genome with a T7 promoter and single transfection to efficiently rescue the virus. The rescued virus showed similar characteristics to the parental virus, and this approach circumvents the difficulty in in vitro transcription of long linear DNA.
ARCHIVES OF VIROLOGY
(2021)
Article
Virology
Stefano Fontana, Daniela Cimini, Katia Marinelli, Giada Gori, Vania Moroni, Patrizia Bagnarelli, Lucia Collini, Elisabetta Pagani, Elisa Masi, Gabriele Buttinelli, Stefano Fiore, Concetta Amato, Valter Carraro, Paola Stefanelli
Summary: The investigation in Italy found a homogenous laboratory capacity for non-polio enterovirus infection diagnosis, with two outbreaks of Echovirus 30 identified in 2019. These results highlight the need to monitor the circulation of non-polio enterovirus to ascertain the real burden of the disease in the country.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Optics
Vincent Hahn, Pascal Rietz, Frank Hermann, Patrick Mueller, Christopher Barner-Kowollik, Tobias Schloeder, Wolfgang Wenzel, Eva Blasco, Martin Wegener
Summary: In recent years, there has been a growing interest in high-speed, high-resolution optics-based 3D printing technology. By combining image projection with optical nonlinearity, researchers have successfully achieved high printing rates with small voxel volumes.
Article
Plant Sciences
Junqiang He, Wenyu Xiong, Lina Zhao, Bin Liu, Ying Huang
Summary: This study isolated eight compounds from G. frondosa and investigated their bioactivity against α-glucosidase, tumor cells, and EV71. Some of the compounds showed inhibitory effects on α-glucosidase, tumor cell proliferation, and EV71 replication, suggesting their potential in treating diabetes, cancer, and EV71 infection.
PLANT FOODS FOR HUMAN NUTRITION
(2023)
Article
Chemistry, Inorganic & Nuclear
Meilin Tao, Qiushi Yin, Alexey L. Kaledin, Natalie Uhlikova, Xinlin Lu, Ting Cheng, Yu-Sheng Chen, Tianquan Lian, Yurii Geletii, Djamaladdin G. Musaev, John Bacsa, Craig L. Hill
Summary: This study focuses on the characterization of a mixed 3d metal oxide water oxidation catalyst using synchrotron XRAS. The results showed that the catalyst has a higher water oxidation activity compared to its isostructural analogue. Multiple lines of evidence confirmed the principal WOC and provided insights into the reaction mechanism.
INORGANIC CHEMISTRY
(2022)
Article
Virology
Zening Yang, Huiwen Zheng, Heng Li, Yanli Chen, Dongpei Hou, Qiqi Fan, Jie Song, Lei Guo, Longding Liu
Summary: During EV-D68 replication, the interaction between 3D(Poly) and PGAM5 affects mitochondrial dynamics and inhibits the expression of IFN-beta by impacting the RIG-I-like receptor signal pathway.
Article
Biochemistry & Molecular Biology
Calvin Yeager, Griffin Carter, David W. Gohara, Neela H. Yennawar, Eric J. Enemark, Jamie J. Arnold, Craig E. Cameron
Summary: This study elucidates the first biochemical mechanism of the enteroviral 2C protein and confirms its applicability to other members of the enterovirus genus. The research shows that RNA binds to the 2C protein driven by the backbone, stimulating ATPase activity. This provides an important biochemical description of the determinants driving specificity and catalytic efficiency of the picornaviral 2C ATPase.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Chemistry, Multidisciplinary
Inacrist Geronimo, Pietro Vidossich, Elisa Donati, Marco De Vivo
Summary: DNA and RNA polymerases play crucial roles in genetic information transfer, with implications for human diseases and biotechnological applications. Computational studies have advanced understanding of polymerase function and could potentially accelerate drug discovery and engineering of artificial polymerases.
WILEY INTERDISCIPLINARY REVIEWS-COMPUTATIONAL MOLECULAR SCIENCE
(2021)
Article
Chemistry, Physical
Gang Wang, Lili Zhu, Xiaoxiao Wu, Zhenyu Qian
Summary: This study focuses on the inhibitory effect of norepinephrine (NE) on the oligomerization of alpha-synuclein (aS) in Parkinson's disease. Molecular dynamics simulations showed that both protonated and nonprotonated NE can lower the critical temperature required for aS fibril decomposition and prevent the formation of compact aS oligomers by disrupting hydrogen bonds and side-chain packing.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Biochemistry & Molecular Biology
Stephanie L. Johnson, Geeta J. Narlikar
Summary: In this study, a single molecule biophysical assay was used to investigate the mechanism of chromatin remodelers. The results showed that ATP hydrolysis by the molecular motors promoted coordination and played a key role in directional sliding.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Computer Science, Artificial Intelligence
Shaolun Sun, Zejun Yu, Sen Zhang, Wendong Xiao, Yongliang Yang
Summary: This paper establishes a mechanism model based on the charging operation to predict the burden profile. The Gaussian process regression algorithm is used to fuse the model with radar detection data for better reconstruction. Furthermore, the traditional shape of a burden surface is studied based on phased array radar point cloud data, and 4 classes of burden surfaces are defined and reconstructed. The reconstructed burden surface is classified using expert-defined features and deep features extracted by convolutional neural networks (CNNs).
EXPERT SYSTEMS WITH APPLICATIONS
(2023)
Article
Engineering, Environmental
Fei Xu, Peng Chen, Hao Li, Suyu Qiao, Jiaxin Wang, Ying Wang, Xitong Wang, Bohan Wu, Huangkang Liu, Can Wang, Heng Xu
Summary: Through comparative transcriptome analysis, differences in the response of Pleurotus ostreatus and Pleurotus cornucopiae to Cd contamination were identified, including differences in Cd accumulation, removal rates, and expression of differentially expressed genes. Functional categories and pathways involved in Cd detoxification also differed between the two species, providing new insights into the molecular mechanisms of mycoremediation and accumulator screening.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Jia-Yue Wang, Jing-Xin Li, Jing Ning, Xiao-Kui Huo, Zhen-Long Yu, Yan Tian, Bao-Jing Zhang, Yan Wang, Deng Sa, Ya-Chen Li, Xia Lv, Xiao-Chi Ma
Summary: This study investigates the oxidative metabolism of Dimethomorph (DMM) mediated by the human cytochrome P450 enzyme (CYP) and its toxicological effects. The study reveals that DMM is mainly metabolized through a two-step oxidation process mediated by CYP3A, and can cause mechanism-based inactivation (MBI) of CYP3A. Additionally, other common fungicides can inhibit the metabolism of DMM, leading to increased exposure to DMM in vivo.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Applied
Jun Wang, Luyi Zhou, Congying Rao, Guang-Li Wang, Feng Jiang, Ayushi Singh, Abhinav Kumar, Jianqiang Liu
Summary: Two new Zn(II) metal-organic-frameworks with supramolecular isomerism have been synthesized using different ligands and display efficient photocatalytic properties for the degradation of model aromatic dye. Density of states calculations explain the photocatalytic mechanism in both frameworks, while chromatography-mass spectrometry further validates the potential photodegradation mechanism.
Article
Cell Biology
Shuai Wang, Zongyang Qiu, Yingnan Hou, Xiya Deng, Wei Xu, Tingting Zheng, Peihan Wu, Shaofang Xie, Weixiang Bian, Chong Zhang, Zewei Sun, Kunpeng Liu, Chao Shan, Aifu Lin, Shibo Jiang, Youhua Xie, Qiang Zhou, Lu Lu, Jing Huang, Xu Li
Summary: This study found that AXL may serve as a novel receptor for SARS-CoV-2, playing an important role in promoting viral infection of the human respiratory system. AXL expression level is closely correlated with SARS-CoV-2 S level and could be a potential target for future clinical intervention strategies.
Article
Multidisciplinary Sciences
Jianying Liu, Yang Liu, Chao Shan, Bruno T. D. Nunes, Ruimei Yun, Sherry L. Haller, Grace H. Rafael, Sasha R. Azar, Clark R. Andersen, Kenneth Plante, Nikos Vasilakis, Pei-Yong Shi, Scott C. Weaver
Summary: The Zika virus (ZIKV) emerged from Africa and spread to Asia before causing recent epidemics in the South Pacific and the Americas. Evolutionary analyses revealed that four mutations, which occurred just before ZIKV introduction to the Americas, are direct reversions of previous mutations that accompanied earlier spread from Africa to Asia. Experimental infections showed that the original mutations reduced fitness for urban transmission, while the reversions restored fitness, likely increasing epidemic risk.
NATURE COMMUNICATIONS
(2021)
Article
Medicine, Research & Experimental
Sharmila Nair, Luciano Mazzoccoli, Arijita Jash, Jennifer Govero, Sachendra S. Bais, Tong Hu, Camila R. Fontes-Garfias, Chao Shan, Hideho Okada, Sujan Shresta, Jeremy N. Rich, Pei-Yong Shi, Michael S. Diamond, Milan G. Chheda
Summary: Zika virus targets GBM stem cells and prevents tumor growth by recruiting CD8(+) T cells and myeloid cells. While anti-PD-1 antibody alone moderately improves survival, combining it with ZIKV treatment significantly increases survival rate. Immune-sensitized ZIKV strain shows promise for safe and effective combination therapy with immunotherapies for GBM patients.
Article
Infectious Diseases
Pei Xu, Junling Gao, Chao Shan, Tiffany J. Dunn, Xuping Xie, Hongjie Xia, Jing Zou, Beatriz H. Thames, Amulya Sajja, Yongjia Yu, Alexander N. Freiberg, Nikos Vasilakis, Pei-Yong Shi, Scott C. Weaver, Ping Wu
Summary: Research has shown that inhibiting the overactivated innate immune responses can ameliorate Zika virus-induced neurogenesis reduction in human neural stem cells. Coordinating the host innate immune responses in neural stem cells after ZIKV infection could be a promising therapeutic approach to attenuate ZIKV-associated neuropathology.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Immunology
Awadalkareem Adam, Camila R. Fontes-Garfias, Vanessa V. Sarathy, Yang Liu, Huanle Luo, Emily Davis, Wenqian Li, Antonio E. Muruato, Binbin Wang, Renat Ahatov, Yoseph Mahmoud, Chao Shan, Samantha R. Osman, Steven G. Widen, Alan D. T. Barrett, Pei-Yong Shi, Tian Wang
Summary: The study generated a stable and attenuated ZIKV mutant, ZE4B-36, with combined mutations in E glycosylation and NS4B P36, showing significant reduction in neuroinvasiveness and neurovirulence, as well as low infectivity in mosquitoes. The mutant induced strong immune responses in mice and protected against ZIKV-induced diseases and vertical transmission, suggesting it as a candidate live attenuated vaccine with increased safety and strong immunogenicity for ZIKV prevention and control.
Article
Pharmacology & Pharmacy
Meijun Liu, Binghong Xu, Yuying Ma, Luqing Shang, Sheng Ye, Yaxin Wang
Summary: This study demonstrated the effective antiviral activity of cyanoacrylate and Boc-protected cyanoarylamide derivatives as inhibitors against EV71, showing broad antiviral effects across various cell lines and viral strains. The binding pockets between these inhibitors and 3C(pro) were elucidated through docking studies, providing valuable insights for the development of clinically effective medicines to treat EV71 infections.
ANTIVIRAL RESEARCH
(2021)
Article
Microbiology
Jannyce G. C. Nunes, Bruno T. D. Nunes, Chao Shan, Adriana F. Moraes, Tais R. Silva, Maria H. R. de Mendonca, Liliane L. das Chagas, Franco A. e Silva, Raimunda S. S. Azevedo, Eliana V. P. da Silva, Livia C. Martins, Jannifer O. Chiang, Livia M. N. Casseb, Daniele F. Henriques, Pedro F. C. Vasconcelos, Rommel M. R. Burbano, Pei-Yong Shi, Daniele B. A. Medeiros
Summary: RVNT has been used as an alternative to PRNT for DENV and ZIKV, showing comparable performance to PRNT for DENV Rluc assay and significantly better performance for ZIKV RVNT90. However, ZIKV RVNT90 alone may not be sufficient for diagnosing secondary infections in areas with multiple flaviviruses circulating, suggesting the need for a panel including other circulating flaviviruses.
Article
Biochemistry & Molecular Biology
Yaxin Wang, Binghong Xu, Sen Ma, Hao Wang, Luqing Shang, Cheng Zhu, Sheng Ye
Summary: This study presents the design and examination of five new inhibitors targeting the 3C-Like protease of SARS-CoV-2. The inhibitors showed nanomolar inhibitory capacity and reduced viral loads, highlighting their potential as broad-spectrum antiviral drugs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Xi Yu, Chao Shan, Yibin Zhu, Enhao Ma, Jinglin Wang, Penghua Wang, Pei-Yong Shi, Gong Cheng
Summary: The study found that a T to A mutation in the Zika virus made it easier for the virus to be transmitted by mosquitoes and infect human cells and immunodeficient mice, facilitating the maturation of structural proteins and the formation of infectious viral particles.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Binghong Xu, Meijun Liu, Sen Ma, Yuying Ma, Si Liu, Luqing Shang, Cheng Zhu, Sheng Ye, Yaxin Wang
Summary: EV71 is a major cause of HFMD, but there are no effective clinical agents for its treatment. Two inhibitors of EV71 3C(pro), FOPMC and FIOMC, showed potent antiviral activity and improved metabolic stability. The substitution of an acyl cyanohydrin group with 4-iminooxazolidin-2-one makes FOPMC and FIOMC promising anti-EV71 drug candidates.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Xilin Wu, Yaxing Wang, Lin Cheng, Fengfeng Ni, Linjing Zhu, Sen Ma, Bilian Huang, Mengmeng Ji, Huimin Hu, Yuncheng Li, Shijie Xu, Haixia Shi, Doudou Zhang, Linshuo Liu, Waqas Nawaz, Qinxue Hu, Sheng Ye, Yalan Liu, Zhiwei Wu
Summary: Current COVID-19 vaccines take at least one month to become effective and around 51% of the global population is still not fully vaccinated. This study introduces a nanobody called Nb22 which has ultrapotent neutralization against the Delta variant and can remain in the respiratory system for a long period of time. Intranasal administration of Nb22 provides instantaneous short-term prophylaxis against SARS-CoV-2.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Medicinal
Hao Wang, Rongjuan Pei, Xin Li, Weilong Deng, Shuai Xing, Yanan Zhang, Chen Zhang, Shuai He, Hao Sun, Shuqi Xiao, Jin Xiong, Yecheng Zhang, Xinwen Chen, Yaxin Wang, Yu Guo, Bo Zhang, Luqing Shang
Summary: COVID-19 caused by SARS-CoV-2 has resulted in millions of infections and deaths worldwide. Researchers have identified the 3CL protease of SARS-CoV-2 as a potential target for developing drugs. They have successfully designed peptidomimetic inhibitors with excellent antiviral activity against SARS-CoV-2, indicating their potential as drug candidates.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Jing Zhang, Bin Wang, Xiaoxiao Gao, Cheng Peng, Chao Shan, Silas F. Johnson, Richard C. Schwartz, Yong-Hui Zheng
Summary: Virus infection affects cellular proteostasis and can downregulate viral protein expression to increase viral fitness. This study also reveals unexpected crosstalk among components of the proteostasis network.
NATURE COMMUNICATIONS
(2022)
Article
Public, Environmental & Occupational Health
Ran Wang, Meng Zhang, Linlin Zhang, Mengjia Liu, Chao Shan, Jing An, Zhengde Xie
Summary: This study examined the immune responses of memory T cells to ZIKV in children who received the Japanese encephalitis (JE) vaccine. The results showed that cross-reactive CD8+ central memory T cells secreted IFN-gamma in response to ZIKV, while CD8+ effector memory T cells did not show significant upregulation of functional factors. In the presence of ZIKV, IFN-gamma and TNF-alpha expression was upregulated by CD4+ effector memory T cells, and the expression signature of CD4+ central memory T cells indicated a more cytotoxic potential.
JOURNAL OF TROPICAL MEDICINE
(2022)
Article
Pharmacology & Pharmacy
Sai Shi, Lei Xie, Sen Ma, Binghong Xu, Hailong An, Sheng Ye, Yaxin Wang
Summary: Hand, foot, and mouth disease (HFMD) is a common childhood infectious disease caused by EV infection. We identified Salvianolic acid A (SA) from a traditional Chinese medicine library as a potent inhibitor of EV71 virus infection. Molecular dynamics simulations showed that SA can bind to E71 and disrupt its catalytic center. These findings suggest that SA is a promising candidate for clinical studies as an EV71 3C(pro) inhibitor.
FRONTIERS IN PHARMACOLOGY
(2023)