Article
Chemistry, Analytical
Simone Fortunati, Ilaria Vasini, Marco Giannetto, Monica Mattarozzi, Alessandro Porchetta, Alessandro Bertucci, Maria Careri
Summary: This study provides design rules for programming dynamic DNA structures on the surface of single-walled carbon nanotube electrodes and demonstrates the measurement of current signal to monitor specific DNA interactions. The use of pyrene as a backfilling agent reduces nonspecific adsorption and enables controlled formation of DNA duplexes on the electrode surface. Additionally, the development of amperometric hybridization platforms based on small molecule melamine templated DNA structures is reported.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Xiaotong Meng, Danny O'Hare, Sylvain Ladame
Summary: With the increasing demand for a new generation of nucleic acid tests, easily miniaturized electrochemical sensors show the greatest promise as they can be cost-effective, highly sensitive, and specific. These sensors rely on the immobilization of sequence-specific hybridization probes onto conductive materials or electrodes. This review covers various strategies for immobilizing nucleic acid probes onto different solid electrode materials, including both physical and chemical immobilization techniques.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Immunology
Lan Yang, Youcui Zhang, Wenyanbo Yi, Xue Dong, Mengwei Niu, Yingjie Song, Yao Han, Hao Li, Yansong Sun
Summary: In this study, an efficient screening platform for antiviral crRNA was established using CRISPR-Cas13a nucleic acid detection. The results showed that the screened crRNAs could effectively inhibit viral RNA in mammalian cells and the platform was more accurate than RNA secondary structure prediction. Additionally, the feasibility of the platform was validated by targeting NS gene of influenza A virus (H1N1).
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Optics
Changyu Shen, Xiaoman Chen, Zhenlin Huang, Zhihao Wang, Jiaxiang Liu, Huitong Deng, Dejun Liu, Fangzhou Shu
Summary: This study proposed a label-free optical fiber Mach-Zehnder interferometer (MZI) based biosensor for the specificity detection of single-stranded nucleic acid (NA). The MZI was made of a fiber sandwich structure by core-offset fusion splicing. By chemically modifying the optical fiber surface and fixing the single-stranded probe NA with a known sequence using the covalent fixation method, the specificity of complementary target NA could be detected through the changing of the MZI spectra.
OPTICS AND LASER TECHNOLOGY
(2022)
Article
Biophysics
Hongyan Yu, Qinli Pu, Zhi Weng, Xi Zhou, Junjie Li, Yujun Yang, Wang Luo, Yongcan Guo, Huajian Chen, Ding Wang, Guoming Xie
Summary: A novel m6A-sensitive DNAzyme was utilized with a three-way junction-mediated isothermal exponential CRISPR amplification reaction to directly detect m6A, providing a new platform for potential clinical applications in RNA analysis.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Biotechnology & Applied Microbiology
Ziyu He, Changsheng Liu, Zhongyu Li, Zhou Chu, Xiang Chen, Xupeng Chen, Yuan Guo
Summary: The outbreak of COVID-19 has highlighted the importance of quick and easy testing. Point-of-care testing (POCT) devices offer convenience, speed, and timely results, attracting strong interest from researchers. However, there are still challenges in the development of POCT devices, such as sample pretreatment, detection sensitivity, and specificity. The unique properties of nanomaterials, such as magnetic, optical, thermal, and electrically conductive features, are expected to address these deficiencies and improve the performance of POCT devices.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Anna Nemudraia, Artem Nemudryi, Murat Buyukyoruk, Andrew M. Scherffius, Trevor Zahl, Tanner Wiegand, Shishir Pandey, Joseph E. Nichols, Laina N. Hall, Aidan McVey, Helen H. Lee, Royce A. Wilkinson, Laura R. Snyder, Joshua D. Jones, Kristin S. Koutmou, Andrew Santiago-Frangos, Blake Wiedenheft
Summary: This study applies type-III CRISPR-based RNA capture and concentration technology to the detection of SARS-CoV-2, with advantages of rapidity, high sensitivity, and low cost.
NATURE COMMUNICATIONS
(2022)
Letter
Biotechnology & Applied Microbiology
Long-Can Mei, Ge-Fei Hao, Guang-Fu Yang
Summary: This article reviews the existing thermodynamic databases for protein-nucleic acid interactions and provides general comments to enhance browsing and exploration.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zupeng Wang, Caihong Zhong
Summary: Cas12c-DETECTOR is a new nucleic acid detection platform with high sensitivity and specificity, capable of specifically identifying SNPs through coupling with sgRNAs, and can be used for diagnosing human and plant pathogens in conjunction with other techniques.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Plant Sciences
Bairu Ren, Yi Wang, Huijie Chen, Lei Diao, Jiaxin Wang, Shuoyuan Zhang, Yongzhe Zhang, Meiping Zhang, Rui Yin, Yanfang Wang
Summary: A PCR-NAS rapid detection technique was established for field detection of the pathogen B. cinerea in ginseng. The technique showed higher sensitivity and specificity compared to traditional PCR-electrophoresis. It offers a rapid and cost-effective solution for early detection of the pathogen in ginseng production.
Article
Biochemical Research Methods
Dalin He, Gang Liu, Jing Yang, Xiaoning Jiang, Hongzhi Wang, Yaru Fan, Shangyu Gong, Feng Wei, Youxiang Diao, Yi Tang
Summary: This study developed a recombinase polymerase amplification (RPA)-based CRISPR-Cas13a method for fast detection of TMUV infection. The method showed high specificity and sensitivity, and performed comparably to the standard method in clinical samples.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Medicine, Research & Experimental
Si-Ping Han, Lisa Scherer, Matt Gethers, Ane M. Salvador, Marwa Ben Haj Salah, Rebecca Mancusi, Sahil Sagar, Robin Hu, Julia DeRogatis, Ya-Huei Kuo, Guido Marcucci, Saumya Das, John J. Rossi, William A. Goddard
Summary: This study achieved the development of siRNA pro-drugs based on nucleic acid switches, which selectively interfere with target genes through specific RNA biomarkers. It demonstrated potential clinical applications in rat cardiomyocytes, providing a new avenue for the treatment of heart diseases.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Public, Environmental & Occupational Health
N. M. Pollak, G. A. Marsh, M. Olsson, D. McMillan, J. Macdonald
Summary: This study developed a low-resource rapid Hendra virus detection method, which combined fast and simple sample processing with RT-RPA technology and LFD technology. The results were obtained in 30 minutes and only required basic equipment for handling. The test showed high sensitivity and specificity and could provide a safe testing alternative outside of the laboratory.
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Review
Food Science & Technology
Rong Du, Xiebingqing Yang, Ping Jin, Yahui Guo, Yuliang Cheng, Hang Yu, Yunfei Xie, He Qian, Weirong Yao
Summary: This review summarizes the recent 5-year research progress on the G-quadruplex (G4) structure in the detection of food contaminants. The article discusses the different applications of G4 as a recognition unit and a signal reporter, highlighting the current challenges and future directions, providing valuable insights for the field of food safety.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)