期刊
JOURNAL OF VIROLOGY
卷 86, 期 18, 页码 9828-9842出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JVI.00677-12
关键词
-
类别
资金
- U.S. Public Health Service [GM 061515-05A2/G120CD, GM 061515-07S1]
- NSF [MCB 1157906]
- Frederick National Laboratory for Cancer Research, National Institutes of Health [HHSN 261200800001E]
- Intramural Research Program of the National Institutes of Health, Frederick National Laboratory for Cancer Research, Center for Cancer Research
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1157906] Funding Source: National Science Foundation
Many plant RNA viruses contain elements in their 3' untranslated regions (3' UTRs) that enhance translation. The PTE (Panicum mosaic virus-like translational enhancer) of Pea enation mosaic virus (PEMV) binds to eukaryotic initiation factor 4E (eIF4E), but how this affects translation from the 5' end is unknown. We have discovered a three-way branched element just upstream of the PEMV PTE that engages in a long-distance kissing-loop interaction with a coding sequence hairpin that is critical for the translation of a reporter construct and the accumulation of the viral genome in vivo. Loss of the long-distance interaction was more detrimental than elimination of the adjacent PTE, indicating that the RNA-RNA interaction supports additional translation functions besides relocating the PTE to the 5' end. The branched element is predicted by molecular modeling and molecular dynamics to form a T-shaped structure (TSS) similar to the ribosome-binding TSS of Turnip crinkle virus (TCV). The PEMV element binds to plant 80S ribosomes with a K-d (dissociation constant) of 0.52 mu M and to 60S subunits with a K-d of 0.30 mu M. Unlike the TCV TSS, the PEMV element also binds 40S subunits (K-d, 0.36 mu M). Mutations in the element that suppressed translation reduced either ribosome binding or the RNA-RNA interaction, suggesting that ribosome binding is important for function. This novel, multifunctional element is designated a kl-TSS (kissing-loop T-shaped structure) to distinguish it from the TCV TSS. The kl-TSS has sequence and structural features conserved with the upper portion of most PTE-type elements, which, with the exception of the PEMV PTE, can engage in similar long-distance RNA-RNA interactions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据