Article
Microbiology
Ramon Antano-Arias, Oscar Del Moral-Hernandez, Julio Ortiz-Ortiz, Luz Del Carmen Alarcon-Romero, Jorge Adan Navor-Hernandez, Marco Antonio Leyva-Vazquez, Marco Antonio Jimenez-Lopez, Jorge Organista-Nava, Berenice Illades-Aguiar
Summary: This study identified specific E7 variants associated with the highest risk of cervical cancer, with E6/E7 bicistron analysis revealing even greater risk potential for specific variants. These findings underscore the importance of analyzing combinations of E6/E7 variants in HPV 16 infection for predicting cervical cancer development.
Article
Biochemistry & Molecular Biology
Diego Carrillo-Beltran, Julio C. Osorio, Rances Blanco, Carolina Oliva, Enrique Boccardo, Francisco Aguayo
Summary: Although epidemiological studies suggest that tobacco smoking and HR-HPV infection are mutually exclusive risk factors for developing HNC, some individuals are both HPV-positive and smokers. Both factors increase oxidative stress and DNA damage. This study found that oral cells expressing HPV16 E6/E7 oncoproteins exposed to cigarette smoke condensate showed synergistic increases in SOD2 levels and DNA damage, independent of Akt1 and ATM. These findings suggest that the interaction between HPV and cigarette smoke in HNC promotes SOD2 alterations, leading to increased DNA damage and the development of a distinct clinical entity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Shuo Li, Mengxia Ye, Yonglin Chen, Quan Gong, Bing Mei
Summary: This study sheds light on the relationship between HPV-52 and cervical cancer, identifying variations in the E6-E7 genes and analyzing the protein's structure and selective pressure. Multiple single nucleotide variants were found in the E6 and E7 sequences of HPV-52, some impacting B cell epitopes.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Nicole Brimer, Scott Vande Pol
Summary: This study reveals that the enhanced competitiveness of HPV16-infected cells is primarily due to the expression of the E6 oncoprotein, rather than the E7 or E5 oncoproteins. E6 can induce a super-competitor phenotype, which has important implications for understanding the mechanism of cell competition.
Review
Biotechnology & Applied Microbiology
Qiu Peng, Lujuan Wang, Liang Zuo, Shuichao Gao, Xianjie Jiang, Yaqian Han, Jinguan Lin, Mingjing Peng, Nayiyuan Wu, Yanyan Tang, Hao Tian, Yujuan Zhou, Qianjin Liao
Summary: HPV infection is closely associated with the development of malignancies, such as cervical, anal, and oral cancers. The viral oncoproteins E6 and E7 play crucial roles in carcinogenesis by affecting various signaling pathways. These proteins promote malignant transformation and tumor progression by regulating downstream tumor-related signaling pathways.
CANCER GENE THERAPY
(2023)
Article
Virology
Kerry Dust, Michael Carpenter, Julie Chih-yu Chen, Chris Grant, Stuart McCorrister, Garret R. Westmacott, Alberto Severini
Summary: This study investigates the effects of HPV16 viral proteins E6 and E7 on the host proteome in human neonatal keratinocytes. The results show that the expression of E6 and E7 leads to dysregulation in pathways related to interferon response, inflammation, and DNA damage repair. The study also identifies potential biomarkers associated with HPV oncoproteins in primary keratinocytes.
Article
Virology
Sebastian O. Wendel, Avanelle Stoltz, Xuan Xu, Jazmine A. Snow, Nicholas Wallace
Summary: A subset of human papillomaviruses (HPVs), particularly HPV16, play a crucial role in the development of cervical cancer. The HPV16 E7 oncogene destabilizes RB and facilitates viral replication, while HPV16 E6 degrades p53 to mitigate replication stress. This study shows that cervical cancer cells activate the translesion synthesis (TLS) pathway to prevent replication fork collapse caused by stress. Increased expression of TLS genes is more common in cervical cancer than in other cancer types, and is associated with improved survival rates.
Article
Immunology
Carolina Olivera, Jessica P. Mosmann, Ailen N. Anna, Gloria N. Bettucci Ferrero, Daniela A. Paira, Fernando N. Ferreyra, Maria S. Martinez, Ruben D. Motrich, Cecilia G. Cuffini, Hector Alex Saka, Virginia E. Rivero
Summary: Coinfection of HPV and CT can lead to increased expression of immune inhibitory molecules and oncogenic proteins, as well as reduced severity and infectivity of CT infection. However, the specific effects of HPV infection on CT development are still not well understood.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Virology
Sadhana M. Gupta, Himangi Warke, Hemangi Chaudhari, Padmaja Mavani, Rajshree D. Katke, Shilpa C. Kerkar, Jayanti Mania-Pramanik
Summary: This study aimed to evaluate HPV E6/E7 oncogene expression as markers for early identification of cervical cancer risk in women with minor cytological abnormalities and negative cytology. The results showed that E6 and E7 transcripts could serve as sensitive early predictors of cervical cancer risk.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Review
Virology
James C. Romero-Masters, Paul F. Lambert, Karl Munger
Summary: HPVs cause a wide range of human diseases, from benign warts to malignant cancers. The discovery of a murine papillomavirus that infects laboratory mice provides an opportunity to study HPV-induced malignant diseases. The E6 and E7 proteins encoded by high-risk HPVs contribute to HPV-induced cancers in multiple ways.
Article
Virology
Mengxia Ye, Shuo Li, Ping Luo, Xuan Tang, Quan Gong, Bing Mei
Summary: This study analyzed the variations and phylogenetic relationship of HPV-51 virus in central China, as well as predicted its immune epitopes. The results showed high prevalence and genetic variations of HPV-51 in the region. These findings are of great importance for future research on vaccines with wider coverage and the development of diagnostic probes.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Katsuyoshi Kumagai, Masakatsu Takanashi, Shin-ichiro Ohno, Yuichirou Harada, Koji Fujita, Keiki Oikawa, Katsuko Sudo, Shun-ichi Ikeda, Hirotaka Nishi, Kosuke Oikawa, Masahiko Kuroda
Summary: Cervical cancer development involves HPV infection and estrogen receptor signaling. WAPL plays a crucial role in CIN development by activating MACROD1 to increase ESR1 sensitivity, thereby promoting the expression of MYC and Cyclin D1.
Article
Medicine, Research & Experimental
Diane Bruyere, Patrick Roncarati, Alizee Lebeau, Thomas Lerho, Florian Poulain, Elodie Hendrick, Charlotte Pilard, Celia Reynders, Marie Ancion, Margaux Luyckx, Michael Renard, Yves Jacob, Jean-Claude Twizere, Raphael Peiffer, Olivier Peulen, Philippe Delvenne, Pascale Hubert, Alison McBride, Nicolas Gillet, Murielle Masson, Michael Herfs
Summary: HPV-positive cancers are usually associated with a favorable outcome due to high sensitivity to radiation therapy. However, the impact of viral E6/E7 oncoproteins on cellular radiosensitivity and host DNA repair is still largely speculative.
Article
Microbiology
Shiwen Peng, Louise Ferrall, Stephanie Gaillard, Chenguang Wang, Wei-Yu Chi, Chuan-Hsiang Huang, Richard B. S. Roden, T-C Wu, Yung-Nien Chang, Chien-Fu Hung
Summary: Immunotherapy for cervical cancer targeting high-risk human papillomavirus types 16 and 18 shows promising results in preclinical studies, with pBI-11 DNA vaccine inducing strong immune responses and therapeutic effects in mouse models. Combination with TA-HPV boost and PD-1 antibody blockade improves tumor control and survival. Clinical translation of this strategy may provide a new approach for HPV16/18-associated tumors.
Article
Microbiology
Shiwen Peng, Deyin Xing, Louise Ferrall, Ya-Chea Tsai, Richard B. S. Roden, Chien-Fu Hung, T. -C. Wu
Summary: Our research focused on eliminating the confounding effects of dominant HPV antigen presentation through murine MHC-I via epitope mutagenesis, while ensuring oncogenicity. Mutated DNA constructs of HPV16 E6 and E7 were able to abolish murine MHC-I presentation and instead use human HLA-A2 molecules for presentation. These mutated constructs still maintained their oncogenic properties.
Article
Biochemistry & Molecular Biology
Nicola Giangregorio, Annamaria Tonazzi, Cosima Damiana Calvano, Ciro Leonardo Pierri, Giovanna Incampo, Tommaso R. I. Cataldi, Cesare Indiveri
Summary: The effect of mycotoxin patulin on the mitochondrial carnitine/acylcarnitine carrier was investigated. Patulin inhibited the transport function of the carrier in a competitive manner, interacting with a protein region including the substrate-binding area. Site-directed mutagenesis confirmed the key role of the C136 residue in the inhibition of patulin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Tarik Gheit, Richard Muwonge, Eric Lucas, Luisa Galati, Devasena Anantharaman, Sandrine McKay-Chopin, Sylla G. Malvi, Kasturi Jayant, Smita Joshi, Pulikkottil O. Esmy, M. Radhakrishna Pillai, Partha Basu, Rengaswamy Sankaranarayanan, Massimo Tommasino
Summary: This study compared the prevalence of oral HPV infections in women vaccinated with different doses of HPV vaccine and found that three doses of the vaccine were more effective in preventing oral HPV infection compared to a single dose.
Article
Biochemistry & Molecular Biology
Omar Ben Mariem, Simona Saporiti, Uliano Guerrini, Tommaso Laurenzi, Luca Palazzolo, Cesare Indiveri, Maria Barile, Emma De Fabiani, Ivano Eberini
Summary: Riboflavin is a vital water-soluble vitamin that is converted into important cofactors in hundreds of flavoenzymes. Its absorption and distribution are mediated by transmembrane transporters, and mutations in these transporters are associated with certain diseases. The overexpression of these transporters in cancer cells makes them potential targets for drug delivery. In this study, three-dimensional structural models of human riboflavin transporters were proposed, and the interactions between wild-type and mutated transporters with riboflavin were investigated.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2023)
Article
Public, Environmental & Occupational Health
Jean-Marc Kutz, Pia Rausche, Tarik Gheit, Dewi Ismajani Puradiredja, Daniela Fusco
Summary: This review synthesizes the factors influencing the successful implementation of HPV vaccination programs in sub-Saharan Africa in order to improve roll-out and coverage. The review provides an overview and contextualizes the barriers and facilitators of HPV vaccination implementation in 12 countries in sub-Saharan Africa. Lessons learned from past experiences of HPV vaccination in sub-Saharan Africa can contribute to more effective national HPV vaccination programs targeted at eliminating cervical cancer and implementing the WHO triple intervention strategy.
Article
Dermatology
Manuelle Viguier, Corine Perals, Beatrice Poirier, Maxime Battistella, Francois Aubin, Herve Bachelez, Jean-Luc Pretet, Tarik Gheit, Massimo Tommasino, Antoine Touze, Marie-Lise Gougeon, Nicolas Fazilleau
Summary: Lichen planus (LP) is a chronic inflammatory disease characterized by CD8(+) T-cell infiltrate, and HPV16-specific CD8(+) T-cells are involved in different clinical forms of LP. Lichen sclerosus et atrophicus (LSA) has a different immune response, with oligoclonal TCRVss6(+) CTL repertoire.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Alessia Nisco, Tiago M. A. Carvalho, Maria Tolomeo, Daria Di Molfetta, Piero Leone, Michele Galluccio, Milagros Medina, Cesare Indiveri, Stephan Joel Reshkin, Rosa Angela Cardone, Maria Barile
Summary: FLAD1 is crucial for flavin homeostasis and cellular metabolism, and its prognostic significance in pancreatic ductal adenocarcinoma (PDAC) was investigated in this study. The results showed that increased FADS expression/activity is associated with malignancy and stemness.
Article
Biochemistry & Molecular Biology
Chiara Brunocilla, Lara Console, Filomena Rovella, Cesare Indiveri
Summary: In this study, an in silico approach was used to define the transport cycle of LAT1, a transporter overexpressed in certain human cancers. The researchers built new conformational models and analyzed the substrate/protein interaction during the transport cycle. The findings highlight the importance of considering conformational states in in silico analyses and early-stage drug discovery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Luana Lorena Silva Rodrigues, Jose Henrique Pilotto, Katrini Guidolini Martinelli, Alcina F. Nicol, Vanessa Salete De Paula, Tarik Gheit, Nathalia Silva Carlos Oliveira, Carlos Silva-de-Jesus, Vikrant V. Sahasrabuddhe, Diane M. Da Silva, W. Martin Kast, Justin Hardick, Charlotte A. Gaydos, Mariza Goncalves Morgado
Summary: The aim of this study was to classify the diversity of anal HPV and non-HPV sexually transmitted infections (STIs) and compare the concordance between anal and genital infections in HIV-infected and uninfected women living in the Tapajos region, Amazon, Brazil. The overall prevalence of anal HPV infection was high, especially in HIV-infected women. The concordance analysis showed moderate to fair agreement between anal and genital HPV and non-HPV STIs.
Article
Biochemistry & Molecular Biology
Nicola Giangregorio, Annamaria Tonazzi, Lara Console, Mariafrancesca Scalise, Cesare Indiveri
Summary: Summary of the Study:
The interaction between Cys and itaconate was investigated, and it was found that itaconate reacts with CAC causing irreversible inhibition. By lowering the pH, the inhibitory effect of itaconate on CAC was reduced. C136 was identified as the main target of itaconate, and administration of dimethyl itaconate to HeLa cells resulted in inhibition of CAC transport activity.
Article
Biochemistry & Molecular Biology
Daria Di Molfetta, Stefania Cannone, Maria Raffaella Greco, Rosa Caroppo, Francesca Piccapane, Tiago Miguel Amaral Carvalho, Concetta Altamura, Ilaria Saltarella, Diana Tavares Valente, Jean Francois Desaphy, Stephan J. J. Reshkin, Rosa Angela Cardone
Summary: Intracellular pH (pHi) regulation is crucial for the exocrine pancreas, and dysregulation of ion transporters, including bicarbonate transporters and NHE1, is observed in PDAC. The progression of PDAC is facilitated by the acidosis in Collagen-I rich ECM. Our study on organotypic cultures of normal human pancreatic cells (HPDE), parenchymal cancer cells (CPCs), and cancer stem cells (CSCs) reveals that the ECM composition and pHe fluctuations contribute to pHi dysregulation and the presence of NaHCO3 gradients similar to that in tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Walid A. Al-Soneidar, Sam Harper, Francois Coutlee, Tarik Gheit, Massimo Tommasino, Belinda Nicolau
Summary: This study investigated the prevalence of alpha (α), beta (β), and gamma (γ) human papillomaviruses (HPVs) among head and neck cancer (HNC) cases and controls, and their relationship with sociodemographic, behavioral, and oral health factors. The findings showed that the prevalence of α- and γ-HPV was associated with sexual activity, smoking, and drinking habits, while the prevalence of β-HPV was not. Tumor samples mainly included α-HPV, with HPV-16 being the most common genotype.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Virology
Nilanjana Chatterjee, Farhin Sultana, Rituparna Roy, Samyadipta Dey, Sukanya Naskar, Aniruddha Dam, Anup K. Bhowmick, Rakiba Begum, Shyam S. Mandal, Ranajit K. Mandal, Jayanta Chakrabarti, Chinmay K. Panda, Massimo Tommasino, Tarik Gheit, Sankhadeep Dutta
Summary: This study investigated the prevalence of novel-HPV 223 and 225 in oral mucosa and keratinized skin epithelium. HPV 223 was found to be present only in the oral mucosal epithelium, while HPV 225 was found in both oral mucosa and skin epithelium.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Taja Lozar, Aysenur Keske, Racheal S. Dube Mandishora, Qiqi Yu, Adam Bailey, Jin Xu, Massimo Tommasino, Stephanie M. Mcgregor, Paul F. Lambert, Tarik Gheit, Megan B. Fitzpatrick
Summary: This study is the first to report the association between p16-negative vulvar squamous intraepithelial lesions and detection of beta-HPVs. These findings expand possible etiologic mechanisms that may contribute to p16-negative lesions of the vulva.