Review
Biochemistry & Molecular Biology
Juan Garcia-Arriaza, Mariano Esteban, Daniel Lopez
Summary: There is a need for a highly effective vaccine against the emerging chikungunya virus, and this review discusses the development of potential vaccine candidates. It highlights the importance of understanding the immune responses induced by these candidates in animal models before progressing to human clinical trials.
Article
Immunology
Alexandra Kupke, Asisa Volz, Erik Dietzel, Astrid Freudenstein, Joerg Schmidt, Hosam Shams-Eldin, Sylvia Jany, Lucie Sauerhering, Verena Kraehling, Michelle Gellhorn Serra, Christiane Herden, Markus Eickmann, Stephan Becker, Gerd Sutter
Summary: This study tested two recombinant MVA-based Ebola virus vaccine candidates in mice and found that vaccination generated virus-neutralizing antibodies and specific CD8+ T cell response. Vaccinated mice survived Ebola virus infection and had significantly reduced viral loads compared to mock-vaccinated mice.
Letter
Medicine, General & Internal
Aniruddha Hazra, Laura Rusie, Trevor Hedberg, John A. Schneider
Summary: This study utilizes electronic medical records to analyze monkeypox infections following a single dose of Modified Vaccinia Ankara-Bavarian Nordic vaccine, a nonreplicating vaccine used for preventing smallpox and monkeypox infections in adults.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2022)
Article
Virology
Noam Erez, Linda S. Wyatt, Jeffrey L. Americo, Wei Xiao, Bernard Moss
Summary: The study identified spontaneous mutations in MVA that led to increased replication in monkey BS-C-1 cells but minimal effects in human cells, mainly due to amino acid substitutions in the D10 decapping enzyme. Despite the mutations being distant from the active site of the decapping enzyme, engineered mutations still enhanced virus replication in BS-C-1 cells. The impact of these mutations on the immunogenicity of MVA vectors requires further investigation.
JOURNAL OF VIROLOGY
(2021)
Article
Virology
Franziska Tanneberger, Ahmed Abd El Wahed, Melina Fischer, Sandra Blome, Uwe Truyen
Summary: African swine fever poses a global threat to the pig industry and wild suids, with affected wild boar populations in Germany playing a significant role. The study found that MVAV can be used as a guide to select the best disinfectant concentration, with 0.1% peracetic acid effectively inactivating ASFV.
Review
Immunology
Lior Nave, Ili Margalit, Noam Tau, Ido Cohen, Dana Yelin, Florian Lienert, Dafna Yahav
Summary: This study evaluated the safety and immunogenicity of the Modified Vaccinia Ankara (MVA) vaccine. The results provide reassuring evidence of MVA's immunogenicity and safety, but further research is needed to confirm the immunogenicity of a single dose and its clinical effectiveness.
Article
Microbiology
Chae Hong Rhee, Moon Her, Wooseog Jeong
Summary: In South Korea, efficacy-tested, virus-specific disinfectants are allowed for use in the veterinary industry. However, domestic testing of disinfectants against foreign and contagious livestock diseases is legally restricted. To address this issue, the Animal and Plant Quarantine Agency (APQA) conducted a study to find a potential surrogate for African swine fever virus (ASFV) for efficacy testing of disinfectants. They compared the modified vaccinia virus Ankara (MVA) to ASFV in terms of their susceptibility to disinfectants. The study found that the disinfectants are effective against MVA at similar or higher concentrations than those against ASFV, validating the use of MVA as a potential surrogate for ASFV in efficacy testing.
Article
Multidisciplinary Sciences
Taylor Pursell, Jennifer L. Spencer Clinton, Jie Tan, Rongsheng Peng, Paul D. Ling
Summary: EEHV can cause lethal hemorrhagic disease in Asian elephants, and developing a vaccine would significantly improve conservation efforts for this endangered species. Recent studies show that a recombinant virus can induce robust humoral and cell-mediated responses.
Article
Immunology
Matthijs P. Raadsen, Christine Dahlke, Anahita Fathi, Mart M. Lamers, Petra van den Doel, Luca M. Zaeck, Martin E. van Royen, Erwin de Bruin, Reina Sikkema, Marion Koopmans, Eric C. M. van Gorp, Gerd Sutter, Rory D. de Vries, Marylyn M. Addo, Bart L. Haagmans
Summary: A modified vaccinia virus Ankara-based vaccine candidate against Middle East respiratory syndrome-associated coronavirus can produce neutralizing antibodies against monkeypox virus in healthy participants after three doses. Modified vaccinia virus Ankara is used as a vaccine against monkeypox virus and also as a viral vector. MVA-MERS-S is a vaccine candidate against Middle East respiratory syndrome-associated coronavirus. After the first dose of MVA-MERS-S vaccine, only one participant showed cross-reactive monkeypox virus neutralizing antibodies, while three out of ten participants showed them after the second dose, and all ten participants had them after the third dose.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Lucas Wilken, Sonja Stelz, Ayse Agac, Gerd Sutter, Chittappen Kandiyil Prajeeth, Guus F. Rimmelzwaan
Summary: The four serotypes of dengue virus (DENV1-4) remain a major public health threat. The first licensed dengue vaccine has shown poor performance in individuals with low immunity, making them susceptible to antibody-enhanced dengue disease. NS1, a non-structural protein of DENV, has the potential to trigger vascular leakage, a severe symptom of dengue disease, but its use as a vaccine antigen is hindered by this drawback.
Article
Multidisciplinary Sciences
Jeffy G. Mattathil, Asisa Volz, Olusegun O. Onabajo, Sean Maynard, Sandra L. Bixler, Xiaoying X. Shen, Diego Vargas-Inchaustegui, Marjorie Robert-Guroff, Celia Lebranche, Georgia Tomaras, David Montefiori, Gerd Sutter, Joseph J. Mattapallil
Summary: HIV is a virus that causes immunodeficiency and AIDS, and developing vaccines to prevent infection is crucial. We hypothesized that vaccinating mucosa associated lymphoid tissue (MALT) such as the palatine tonsils could overcome the compartmentalization between mucosal and peripheral immune systems. Our study in rhesus macaques showed that priming with plasmid DNA and boosting with MVA in the tonsil MALT protected against SIVmac251 infection, with 43% remaining uninfected after 9 challenges. Vaccination was associated with reduced viremia and enhanced immune responses. This combination approach could provide protection from mucosal infection with highly pathogenic HIV.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Gwendal Graenicher, Felipe Tapia, Ilona Behrendt, Ingo Jordan, Yvonne Genzel, Udo Reichl
Summary: The study compares different cell retention options for high-yield MVA virus production in perfusion mode, finding that the highest yields can be achieved through a perfusion strategy with working volume expansion after virus infection.
BIOTECHNOLOGY JOURNAL
(2021)
Letter
Medicine, General & Internal
Lucy Deng, Laura K. Lopez, Catherine Glover, Patrick Cashman, Renee Reynolds, Kristine Macartney, Nicholas Wood
Summary: This study utilizes data from Australia's vaccine safety surveillance system to investigate the adverse effects of the modified vaccinia Ankara-Bavarian Nordic vaccine.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2023)
Article
Multidisciplinary Sciences
Balazs Kakuk, Akos Dormo, Zsolt Csabai, Gabor Kemenesi, Jiri Holoubek, Daniel Ruzek, Istvan Prazsak, Virag eva Dani, Bela Denes, Gabor Torma, Ferenc Jakab, Gabor E. Toth, Fanni V. Foldes, Brigitta Zana, Zsofia Lanszki, Akos Harangozo, Adam Fulop, Gabor Gulyas, Mate Mizik, Andras Attila Kiss, Dora Tombacz, Zsolt Boldogkoi
Summary: The recent Monkeypox outbreak highlighted the importance of studying the basic biology of orthopoxviruses. However, there has been no investigation into the transcriptome of the causative agent using either short-read or long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap and allows for the comprehensive characterization of the monkeypox virus transcriptomic architecture and the potential annotation of novel host transcripts. Additionally, this study will provide insights into gene expression changes in both the virus and host cells during infection, leading to a deeper understanding of the alterations caused by viral infection at the transcriptome level.
Article
Immunology
Nanda Kishore Routhu, Sailaja Gangadhara, Lilin Lai, Meredith E. Davis-Gardner, Katharine Floyd, Ayalnesh Shiferaw, Yannic C. Bartsch, Stephanie Fischinger, Georges Khoury, Sheikh Abdul Rahman, Samuel David Stampfer, Alexandra Schafer, Sherrie M. Jean, Chelsea Wallace, Rachelle L. Stammen, Jennifer Wood, Cohen Joyce, Tamas Nagy, Matthew S. Parsons, Lisa Gralinski, Pamela A. Kozlowski, Galit Alter, Mehul S. Suthar, Rama Rao Amara
Summary: The study demonstrates that vaccination with MVA/SdFCS-N via the IM route can effectively induce cross-reactive antibody and T cell responses, providing better protection against heterologous SARS-CoV-2 VOCs compared to other vaccination routes.
SCIENCE IMMUNOLOGY
(2022)
Article
Neurosciences
Ana M. Rodriguez, M. Victoria Delpino, M. Cruz Miraglia, Miriam M. Costa Franco, Paula Barrionuevo, Vida A. Dennis, Sergio C. Oliveira, Guillermo H. Giambartolomei
Article
Cell Biology
Ajithkumar Vasanthakumar, Yang Liao, Peggy Teh, Maria F. Pascutti, Anna E. Oja, Alexandra L. Garnham, Renee Gloury, Jessica C. Tempany, Tom Sidwell, Eloy Cuadrado, Paul Tuijnenburg, Taco W. Kuijpers, Najoua Lalaoui, Lisa A. Mielke, Vanessa L. Bryant, Philip D. Hodgkin, John Silke, Gordon K. Smyth, Martijn A. Nolte, Wei Shi, Axel Kallies
Article
Immunology
Maria Soledad Hielpos, Mariana C. Ferrero, Andrea G. Fernandez, Juliana Falivene, Silvia Vanzulli, Diego J. Comerci, Pablo C. Baldi
FRONTIERS IN IMMUNOLOGY
(2017)
Article
Medicine, General & Internal
Maria Julia Ruiz, Jimena Salido, Lorena Abusamra, Yanina Ghiglione, Cintia Cevallos, Gabriel Damilano, Ana Maria Rodriguez, Cesar Trifone, Natalia Laufer, Luis D. Giavedoni, Omar Sued, Horacio Salomon, Maria Magdalena Gherardi, Gabriela Turk
Letter
Hematology
Sulima Geerman, Giso Brasser, Sudeep Bhushal, Fiamma Salerno, Natasja A. Kragten, Mark Hoogenboezem, Gerald de Haan, Monika C. Wolkers, Maria Fernanda Pascutti, Martijn A. Nolte
Article
Immunology
Maria C. Miraglia, Ana M. Rodriguez, Paula Barrionuevo, Julia Rodriguez, Kwang S. Kim, Vida A. Dennis, M. Victoria Delpino, Guillermo H. Giambartolomei
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Letter
Hematology
Sulima Geerman, Giso Brasser, Sudeep Bhushal, Fiamma Salerno, Natasja A. Kragten, Mark Hoogenboezem, Gerald de Haan, Monika C. Wolkers, Maria Fernanda Pascutti, Martijn A. Nolte
Article
Immunology
Aldana Trotta, Lis N. Velasquez, M. Ayelen Milillo, M. Victoria Delpino, Ana M. Rodriguez, Veronica I. Landoni, Guillermo H. Giambartolomei, Roberto G. Pozner, Paula Barrionuevo
FRONTIERS IN IMMUNOLOGY
(2018)
Article
Immunology
M. Soledad Hielpos, Andrea G. Fernandez, Juliana Falivene, Ivan M. Alonso Paiva, Florencia Munoz Gonzalez, Mariana C. Ferrero, Priscila C. Campos, Angelica T. Vieira, Sergio Costa Oliveira, Pablo C. Baldi
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2018)
Article
Immunology
Maria Fernanda Pascutti, Sulima Geerman, Nicholas Collins, Giso Brasser, Benjamin Nota, Regina Stark, Felix Behr, Anna Oja, Edith Slot, Eleni Panagioti, Julia E. Prier, Sarah Hickson, Monika C. Wolkers, Mirjam H. M. Heemskerk, Pleun Hombrink, Ramon Arens, Laura K. Mackay, Klaas P. J. M. van Gisbergen, Martijn A. Nolte
EUROPEAN JOURNAL OF IMMUNOLOGY
(2019)
Article
Multidisciplinary Sciences
Maria Paula Caruso, Juliana Falivene, Maria Pia Holgado, Diego Hernan Zurita, Natalia Laufer, Carina Castro, Angeles Nico, Cynthia Maeto, Jimena Salido, Hector Perez, Horacio Salomon, Pedro Cahn, Omar Sued, Valeria Fink, Gabriela Turk, Maria Magdalena Gherardi
SCIENTIFIC REPORTS
(2019)
Review
Neurosciences
Ana M. Rodriguez, M. Victoria Delpino, Maria Cruz Miraglia, Guillermo H. Giambartolomei
Article
Immunology
Anna E. Oja, Giso Brasser, Edith Slot, Rene A. W. van Lier, Maria F. Pascutti, Martijn A. Nolte
IMMUNOLOGY LETTERS
(2020)
Article
Microbiology
Ana Maria Rodriguez, Aldana Trotta, Agustina P. Melnyczajko, M. Cruz Miraglia, Kwang Sik Kim, M. Victoria Delpino, Paula Barrionuevo, Guillermo Hernan Giambartolomei
Review
Neurosciences
Ana Maria Rodriguez, Julia Rodriguez, Guillermo Hernan Giambartolomei
Summary: Microglia play both beneficial and detrimental roles in the central nervous system (CNS). In the steady state, they regulate the function of neuronal networks and neurovascular units while providing immune surveillance. However, during CNS infection, immune activation of microglia can generate an inflammatory environment that contributes to pathogen clearance but also harms nearby cells of the CNS.